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A549 cell Next Gen sequencing (RNA-seq) for differential gene expression of untreated versus IL4 or IL4+ALOX15 siRNA treated cells

ABSTRACT: A549 cell Next Gen sequencing (RNA-seq) for differential gene expression of untreated versus IL4 or IL4+ALOX15 siRNA treated cells

PROVIDER: PRJNA720719 | ENA |

REPOSITORIES: ENA

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A549 cell Next Gen sequencing (RNA-seq) for differential gene expression of untreated versus IL4 or IL4+ALOX15 siRNA treated cells

Project description:Purpose: Identify genes regulated by ALOX15 in A549 cells that were treated with +/- IL4 and +/- ALOX15 siRNA by Next-gen sequencing

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Normal human bronchial epithelial cell Next Gen sequencing (RNA-seq) for differential gene expression of untreated versus IL4 or IL4+ALOX15 siRNA treated cells

Project description:Purpose: Identify genes regulated by ALOX15 in Normal human bronchial epithelial (NHBE) cells that were treated with +/- IL4 and +/- ALOX15 siRNA by Next-gen sequencing

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Normal human bronchial epithelial cell Next Gen sequencing (RNA-seq) for differential gene expression of untreated versus IL4 or IL4+ALOX15 siRNA treated cells

Project description:Normal human bronchial epithelial cell Next Gen sequencing (RNA-seq) for differential gene expression of untreated versus IL4 or IL4+ALOX15 siRNA treated cells

| PRJNA743661 | ENA

Comparison of gene expression profiles of A549 cell treated with NRF2 siRNA and control siRNA

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Project description:Untransfected (no siRNA), control siRNA or TOP1 siRNA treated A549 cells were analyzed for gene expression differences under basal conditions

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Analysis of global gene expression profiles of A549 cells treated with XRN2 siRNA under basal conditions

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Analysis of global gene expression profiles of A549 cells treated with SETX siRNA under basal conditions

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Expression data from KEAP1 overexpression and NRF2 siRNA knockdown of A549 NSCLC cell

Project description:KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells were used to identify downstream genes of NRF2 pathway separately and by combinatorial analysis. We used triplicate microarrays of transfected A549 cells with mKeap1-GFP for overexpression, siRNAs targeting NRF2 for knockdown and siGFP as control respectively. As a result, we identified several genes which are involved in cancer metabolic functions in these cells. We used microarrays to identify the gene downregulated in both KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells and found a subset of downregulated genes which are involved in metabolic functions.

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Gene expression (GeneChip) data from scramble- and CRMP2A siRNA-treated A549 cells.

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