Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiles of normal and activity-silenced mitral cells at P3 and P6

ABSTRACT: Gene expression profiles of normal and activity-silenced mitral cells at P3 and P6

PROVIDER: PRJNA721307 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profiles of normal and activity-silenced mitral cells at P3 and P6

Project description:Mitral cells in the mouse olfactory bulb are known to remodel their primary dendrites and form lateral dendrites during postnatal development. These processes are suppressed when neuronal activity is silenced by overexpression of Kir2.1 in mitral cells, indicating that neuronal activity in mitral cells is required. However, it is unclear what changes in gene expression lead to the remodeling of mitral cell dendrites in an activity-dependent manner. To address this, we performed a microarray-based analysis of gene expression in mitral cells during normal development and under the activity-silenced condition.

2021-04-13 | GSE171875 | GEO

Differential Requirement for DICER1 Activity during the Development of Mitral and Tricuspid Vlaves

Project description:Mitral and tricuspid valves are essential for unidirectional blood flow in the heart. They are derived from similar cell sources, and yet congenital dysplasia affecting both valves is clinically rare, suggesting the presence of differential regulatory mechanisms underlying their development. We specifically inactivated Dicer1 in the endocardium during cardiogenesis and found that Dicer1-deletion caused congenital mitral valve stenosis and regurgitation, while it had no impact on other valves. We showed that hyperplastic mitral valves were caused by abnormal condensation and extracellular matrix (ECM) remodeling. Our single-cell RNA Sequencing analysis revealed impaired maturation of mesenchymal cells and abnormal expression of ECM genes in mutant mitral valves. Furthermore, expression of a set of miRNAs that target ECM genes was significantly lower in tricuspid valves compared to mitral valves, consistent with the idea that the miRNAs are differentially required for mitral and tricuspid valve development. We thus reveal miRNA-mediated gene regulation as a novel molecular mechanism that differentially regulates mitral and tricuspid valve development, thereby enhancing our understanding of the non-association of inborn mitral and tricuspid dysplasia observed clinically.

2022-08-19 | GSE207226 | GEO

Clostridioides difficile P6 strain:P6

Project description:Clostridioides difficile P6 strain:P6 Genome sequencing and assembly

| PRJNA85969 | ENA

Cutibacterium acnes P6 strain:P6

Project description:Cutibacterium acnes P6 strain:P6 Genome sequencing and assembly

| PRJNA188768 | ENA

Aureobasidium sp. P6 strain:P6

Project description:Aureobasidium sp. P6 strain:P6 Genome sequencing and assembly

| PRJNA391534 | ENA

Aureobasidium sp. P6 strain:P6

Project description:Aureobasidium sp. P6 strain:P6 Genome sequencing and assembly

| PRJNA509139 | ENA

Normal human mitral valve proteome: a preliminary investigation by gel-based and gel-free proteomic approaches

Project description:The mitral valve is a highly complex structure which regulates blood flow from the left atrium to the left ventricle (LV) avoiding a significant forward gradient during diastole or regurgitation during systole. The integrity of the mitral valve is also essential for the maintenance of normal LV size, geometry, and function. Significant advances in the comprehension of the biological, functional, and mechanical behavior of the mitral valve have recently been made. However, current knowledge of protein components in the normal human mitral valve is still limited and complicated by the low cellularity of this tissue and the presence of high abundant proteins from the extracellular matrix. We employed here an integrated proteomic approach to analyse the protein composition of the normal human mitral valve and reported confident identification of 422 proteins, some of which have not been previously described in this tissue. In particular, we described the ability of pre-MS separation technique based on liquid-phase IEF and SDS-PAGE to identify the largest number of proteins. These initial results provide a valuable basis for future studies aimed at analysing in depth the mitral valve protein composition and at investigating potential pathogenetic molecular mechanisms.

2016-08-18 | PXD004397 | Pride

Mitral Valve Tissue Shotgun Proteomics

Project description:In this study, we carried out a large-scale proteome profiling of human mitral valve tissues resected from patients with mitral valve prolapse.

2015-05-07 | PXD000774 | Pride

Mycobacterium sp. P3 strain:P3

Project description:Mycobacterium sp. P3 strain:P3 Genome sequencing

| PRJNA810296 | ENA

Streptomyces sp. P3 strain:P3

Project description:Streptomyces sp. P3 strain:P3 Genome sequencing

| PRJNA437360 | ENA