Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional Effects of Melatonin of the Gut Commensal Bacterium Klebsiella aerogenes

ABSTRACT: Transcriptional Effects of Melatonin of the Gut Commensal Bacterium Klebsiella aerogenes

PROVIDER: PRJNA721952 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14238400_1.fastq.gz	Fastqsanger.gz
	SRR14238400_2.fastq.gz	Fastqsanger.gz
	SRR14238401_1.fastq.gz	Fastqsanger.gz
	SRR14238401_2.fastq.gz	Fastqsanger.gz
	SRR14238402_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Transcriptional Effects of Melatonin of the Gut Commensal Bacterium Klebsiella aerogenes

Project description:In this study, we implemented RNA sequencing to identify melatonin sensitive transcripts during culture maturation. This work demonstrates that majority of melatonin sensitive genes are growth stage specific. Melatonin caused differential gene expression of 81 transcripts during the exponential growth and 30 during early stationary phase. This indole molecule affects genes related to the biofilm formation, fimbria biogenesis, transcriptional regulators, carbohydrate transport and metabolism, phosphotransferase system (PTS), stress response, metal ion binding and transport. It is likely that differential expression of biofilm and fimbria related genes is responsible for differences in macrocolony area. Additionally, melatonin potentially helps Klebsiella aerogenes in host colonization.

2022-03-23 | GSE172068 | GEO

Differential gene expression of K. aerogenes during temperature entrainment

Project description:In this study we investigate and compare DEGs of K. aerogenes macrocolonies during temperature entrainment (12:12, 35°C:36°C) and subsequent free running conditions (35°C). The goal of this experiment was to identify rhythmically expressed genes in K. aerogenes, the first human gut commensal bacterium with described circadian clock (Paulose et al. 2016). K. aerogenes circadian clock is sensitive to temperature entrainment (Paulose et al. 2019). To further investigate this temperature entrainment of K. aerogenes we performed RNA sequencing experiment. We identified 48 differentially expressed genes when comparing timepoints ZT6 to ZT18, 427 differentially expressed genes when comparing timepoints ZT6 to CT6, 6 differentially expressed genes between timepoints CT6 and CT18, and 2 differentially expressed genes between timepoints ZT18 and CT18.

2023-10-09 | GSE185502 | GEO

Transcriptional effects of melatonin on the gut commensal bacterium Klebsiella aerogenes.

Project description:Klebsiella (nee Enterobacter) aerogenes is the first human gut commensal bacterium with a documented sensitivity to the pineal/gastrointestinal hormone melatonin. Exogenous melatonin specifically increases the size of macrocolonies on semisolid agar and synchronizes the circadian clock of K. aerogenes in a concentration dependent manner. However, the mechanisms driving these phenomena are unknown. In this study, we applied RNA sequencing to identify melatonin sensitive transcripts during culture maturation. This work demonstrates that the majority of melatonin sensitive genes are growth stage specific. Melatonin exposure induced differential gene expression of 81 transcripts during exponential growth and 30 during early stationary phase. This indole molecule affects genes related to biofilm formation, fimbria biogenesis, transcriptional regulators, carbohydrate transport and metabolism, phosphotransferase systems (PTS), stress response, metal ion binding and transport. Differential expression of biofilm and fimbria-related genes may be responsible for the observed differences in macrocolony area. These data suggest that melatonin enhances Klebsiella aerogenes host colonization.

| S-EPMC8934286 | biostudies-literature

Klebsiella aerogenes

Project description:Klebsiella aerogenes

| PRJEB53697 | ENA

Klebsiella aerogenes

Project description:Klebsiella aerogenes Assembly

| PRJNA1026099 | ENA

Anti-inflammatory Effects of the Commensal Bacterium Faecalibacterium prausnitzii analyzed by microarrays

Project description:The human intestinal microbiota plays an essential role in host health. Modifications in its composition and diversity could induce pathologies such as inflammatory bowel diseases (IBD). These diseases are characterized by an unbalanced intestinal microbiota (a process known as dysbiosis) and an altered immune response. Faecalibacterium prausnitzii, the most abundant commensal bacterium in the human intestinal microbiota of healthy individuals (representing more than 5% of the total bacterial population), has been reported to be lower in feces and mucosa-associated microbiota of IBD patients. In addition, we have shown that both F. prausnitzii and its culture supernatant (SN) have anti-inflammatory and protective effects in both acute and chronic colitis models. However, the host molecular mechanisms involved in these anti-inflammatory effects remain unknown. In order to address this issue, we performed DNA chip-based transcriptomic analyses in HT-29 human intestinal epithelial cells stimulated with TNF-a and exposed to F. prausnitzii SN or to BHI (growth medium for F prausnitzii).

2020-11-02 | GSE72048 | GEO

Klebsiella aerogenes

Project description:Klebsiella aerogenes Genome sequencing

| PRJNA1199311 | ENA

Transcription profiling of human bronchial epithelial cells stimulated with the commensal bacterium S. salivarius K12 of the pathogens S. aureus, P. aeruginosa, or S. enteritidis

Project description:Human bronchial epithelial cells (16HBE14O-) were stimulated with the commensal bacterium S. salivarius K12, or the pathogens S. aureus, P. aeruginosa, or S. enteritidis (subtype Typhimurium) for 1 hour.

2008-10-30 | E-FPMI-13 | biostudies-arrayexpress

Klebsiella aerogenes

Project description:Klebsiella aerogenes Raw sequence reads

| PRJNA390808 | ENA

Klebsiella aerogenes

Project description:Klebsiella aerogenes Raw sequence reads

| PRJNA998181 | ENA