Project description:Purpose: The transcriptional alterations underlying physiological changes in fetal alcohol spectrum disorder are largely unidentified. Here we perform RNA sequencing on 0% and 1% EtOH (12 hpf - 5 dpf) zebrafish at 7dpf stages in order to identify significant transcriptional alterations.
Project description:Purpose: The transcriptional alterations underlying physological changes in fetal alcohol spectrum disorder are largely unidentified. Here we perform RNA sequencing on control and embryonic alcohol exposed (EAE/FAE) zebrafish at larval (13 dpf) and adult stages in order to identify significant transcriptional alterations under normal and HFHC diet conditions.
Project description:Gene expression profiles in lri12 mutant zebrafish larvae at 5 dpf based on RNAseq data. This dataset was also used to clone the mutation.
Project description:The present study was conducted in the frame of the EU-funded Graphene Flagship project. The aim is to evaluate the impact of graphene oxide (GO) on the (innate) immune system using zebrafish as a model. We previously performed single-cell RNA-sequencing of germ-free zebrafish embryos exposed to GO plus the microbial metabolite butyrate (BA). Here, we performed a follow up experiment using germ-free lck-GFP transgenic fish in which the zebrafish were exposed to GO plus BA at 5 dpf. The embryos were then dissociated and subsequently sorted on lck and submitted for single-cell RNA-sequencing using 10x Genomics.
Project description:Overexpression of high levels of human UHRF1 tagged with EGFP (hUHRF1-EGFP)high in zebrafish hepatocytes induces cellular senescence by 5 days post fertilization (dpf) and hepatocellular carcinoma by 15-20 dpf. The goal of this study is to compare the transcriptome profile of zebrafish livers with (hUHRF1-EGFP)high overexpression compared to WT siblings. RNAseq was performed on RNA extracted from pools of microdissected liveres by using RiboZero libary prep to capture all transcripts, including transposable elements, that are expressed in the livers.
Project description:Label-free mass spectrometry-based quantitative proteomics was applied to a larval zebrafish spinal cord injury model, which allows axon regeneration and functional recovery within two days (days post lesion; dpl) after a spinal cord transection in 3 day-old larvae (dpf). Proteomic profiling of the lesion site was performed at 1 dpl and 2 dpl as well as corresponding age-matched unlesioned control tissue (4 dpf as control for 1 dpl; 5 dpf as control for 2 dpl).
Project description:In order to search for the downstream target genes of Mafba and Mafbb, we isolated microglia (3 cells per sample) from 3-dpf zebrafish brain of siblings, mafba and mafbb single mutants and DMut under Tg(mpeg1:DsRedx) transgenic background (3 samples per genotype), respectively, and then performed RNA sequencing.