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A role for Elongin in Mediator recruitment at ATF6α regulated genes [RNA-Seq]

ABSTRACT: A role for Elongin in Mediator recruitment at ATF6α regulated genes [RNA-Seq]

PROVIDER: PRJNA722173 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14243144.fastq.gz	Fastqsanger.gz
	SRR14243145.fastq.gz	Fastqsanger.gz
	SRR14243146.fastq.gz	Fastqsanger.gz
	SRR14243147.fastq.gz	Fastqsanger.gz
	SRR14243148.fastq.gz	Fastqsanger.gz

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A role for Elongin in Mediator recruitment at ATF6α regulated genes [RNA-Seq]

Project description:The bZIP transcription factor ATF6α is a master regulator of endoplasmic reticulum (ER) stress response genes. In this report, we identify the multifunctional RNA polymerase II transcription factor Elongin as a cofactor for ATF6α-dependent transcription activation. Biochemical studies reveal that Elongin functions at least in part by facilitating ATF6α-dependent loading of Mediator at the promoters and enhancers of ER stress response genes. Depletion of Elongin from cells leads to impaired transcription of ER stress response genes and to defects in the recruitment of Mediator and, in particular, its CDK8 kinase subunit. Taken together, these findings bring to light a new role for Elongin as a loading factor for Mediator during the ER stress response.

2021-09-01 | GSE172125 | GEO

A role for Elongin in Mediator recruitment at ATF6α regulated genes [Cut&Tag]

2021-09-01 | GSE172132 | GEO

A role for Elongin in Mediator recruitment at ATF6α regulated genes [Cut&Tag]

Project description:A role for Elongin in Mediator recruitment at ATF6α regulated genes [Cut&Tag]

| PRJNA722200 | ENA

Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes

Project description:Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes

| PRJNA722192 | ENA

Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes.

Project description:The bZIP transcription factor ATF6α is a master regulator of endoplasmic reticulum (ER) stress response genes. In this report, we identify the multifunctional RNA polymerase II transcription factor Elongin as a cofactor for ATF6α-dependent transcription activation. Biochemical studies reveal that Elongin functions at least in part by facilitating ATF6α-dependent loading of Mediator at the promoters and enhancers of ER stress response genes. Depletion of Elongin from cells leads to impaired transcription of ER stress response genes and to defects in the recruitment of Mediator and its CDK8 kinase subunit. Taken together, these findings bring to light a role for Elongin as a loading factor for Mediator during the ER stress response.

| S-EPMC8488631 | biostudies-literature

Gene expression profiling of retinoic acid (RA)-treated embryoid bodies (EBs) derived from Elongin A+/+ and Elongin A-/- ES cells.

Project description:To identify potential Elongin A targets during neuronal differentiation of ES cells, a cDNA microarray analysis comparing embryoid bodies (EBs) derived from Elongin A+/+ ES cells and Elongin A-/- ES cells was performed. Gene expression in EBs derived from Elongin A+/+ and Elongin A-/- ES cells was measured at day 4 after retinoic acid treatment (2 ?M).

2011-07-25 | E-GEOD-30911 | biostudies-arrayexpress

Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-08-30 | GSE172134 | GEO

Gene expression profiling of retinoic acid (RA)-treated embryoid bodies (EBs) derived from Elongin A+/+ and Elongin A-/- ES cells.

2011-07-26 | GSE30911 | GEO

EPOP Functionally Links Elongin and Polycomb in Pluripotent Stem Cells

Project description:The cellular plasticity of pluripotent stem cells is thought to be sustained by genomic regions that display both active and repressive chromatin properties. These regions exhibit low levels of gene expression, yet the mechanisms controlling these levels remain unknown. Here, we describe Elongin BC as a binding factor at the promoters of bivalent sites. Biochemical and genome-wide analysis shows that Elongin BC is associated with Polycomb Repressive Complex 2 (PRC2) in pluripotent stem cells. Elongin BC is recruited to chromatin by the PRC2-associated factor EPOP (Elongin- and POlycomb-associated Protein, also termed C17orf96, esPRC2p48, E130012A19Rik), a protein expressed in the inner cell mass of the mouse blastocyst. Both EPOP and Elongin BC are required to maintain low levels of expression at PRC2 genomic targets. Our results indicate that keeping the balance between activating and repressive cues is a more general feature of chromatin in pluripotent stem cells than previously appreciated.

2016-11-21 | GSE79606 | GEO

Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo

Project description:Mediator is a highly conserved transcriptional coactivator organized into four modules, namely Tail, Middle, Head and Kinase (CKM). Previous work suggests regulatory roles for Tail and CKM, but an integrated model for these activities is lacking. Here, we analyzed the genome-wide distribution of Mediator subunits in wild-type and mutant yeast cells in which RNA polymerase II promoter escape is blocked allowing detection of transient Mediator forms. We found that while all modules are recruited to upstream activated regions (UAS), assembly of Mediator within the pre-initiation complex is accompanied by the release of CKM. Interestingly, our data show that CKM regulates Mediator-UAS interaction rather than Mediator-promoter association. In addition, while Tail is required for Mediator recruitment to UAS, Tail-less Mediator nevertheless interacts with core promoters. Collectively, our data suggest that the essential function of Mediator is mediated by Head and Middle at core promoters, while Tail and CKM play regulatory roles.

2016-10-25 | GSE81107 | GEO

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