Project description:Establishment and molecular characterization of 49 peritoneally-metastatic gastric cancer cell lines from 18 patients’ ascites. We performed comprehensive transcriptome analyses using microarrays of our established gastric cancer cell lines.
Project description:In this study, we investigated amplifications and deletions of 49 gastric cancer cell lines by 244k oligonucleotide-based array comparative genomic hybridization.
Project description:Establishment and molecular characterization of 239 peritoneally-metastatic cancer cell lines from 170 patients’ ascites in 11 kinds of cancers mainly consisted of gastric, pancreatic, and ovarian cancer. We performed comparative transcriptome analyses using microarrays between our established cancer cell lines and cell-bank derived cancer cell lines
Project description:Molecular characterization of 7 peritoneally-metastatic gastric cancer cell lines and primary cancer cells established from a patients’ ascites. We performed comprehensive transcriptome analyses using microarrays of our established gastric cancer cell lines and primary cancer cells.
Project description:YTN2 and YTN16 cell lines are subclones established from chemically induced gastric cancer (Yamamoto M et al. Cancer Science 2018). When inoculated to normal C57BL/6 mice, YTN2 spontaneously regresses , while YTN16 grows progressively. To compare expression profiles of these cell lines, we examined microarray analysis.
Project description:As the important layer of the stomach wall, gastric mucosa is related to many functions of digestion. According to the anatomical location, the human stomach is divided into seven regions. In order to get an entire profiling of the 7 regions at proteomic and transcriptomic levels, we collected 7 biopsies from each health volunteer, 49 total were used for integrative analysis, and measured the regional-specific gene products to define the reference intervals of gene expression for outliers screening and prognostic gene detection. Collectively, we presented profiling of normal gastric mucosa at different levels and defined reference ranges of gene expression to study the specific characteristics of abnormal and cancer samples, providing a better understanding of stomach physiological function in studies of gastric disease mechanism.