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Comprehensive characterization of cistrome and epicistrome involved in fruit ripening [red fruit]

ABSTRACT: Comprehensive characterization of cistrome and epicistrome involved in fruit ripening [red fruit]

PROVIDER: PRJNA722537 | ENA |

REPOSITORIES: ENA

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Comprehensive characterization of cistrome and epicistrome involved in fruit ripening

Project description:Comprehensive characterization of cistrome and epicistrome involved in fruit ripening

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Comprehensive characterization of cistrome and epicistrome involved in fruit ripening [pre-experiment]

Project description:Comprehensive characterization of cistrome and epicistrome involved in fruit ripening [pre-experiment]

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Comprehensive characterization of cistrome and epicistrome involved in fruit ripening [ChIP-seq]

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Comprehensive characterization of cistrome and epicistrome involved in fruit ripening [ChIP-seq]

Project description:Here we generated ChIP-seq data of a tomato ERF family TF Sl-ERF_F_4 in red fruit stage and green fruit stage to validate the accuracy of DAP-seq data.

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Comprehensive characterization of cistrome and epicistrome involved in fruit ripening [red fruit]

Project description:The biggest advantage of DAP-seq is that it’s capable of measuring the influence of DNA methylation on TF binding, without disturbing of other epigenetic markers. Here, by utilizing DNA affinity purification sequencing, we generated two binding sets which were incubated with DNA libraries that representing methylomes in different developmental stage. And we made the most of our data to predict DNA methylation impact on TF binding in two dimensions: prediction by comparing genome-wide binding signal in motif-matched regions with or without methylation in single stage, and prediction by comparing binding signal variation in motif-matched regions with or without methylation variation.

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