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Analysis of subcellular transcriptomes by RNA proximity labeling with Halo-seq

ABSTRACT: Analysis of subcellular transcriptomes by RNA proximity labeling with Halo-seq

PROVIDER: PRJNA722745 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14267811_1.fastq.gz	Fastqsanger.gz
	SRR14267811_2.fastq.gz	Fastqsanger.gz
	SRR14267812_1.fastq.gz	Fastqsanger.gz
	SRR14267812_2.fastq.gz	Fastqsanger.gz
	SRR14267813_1.fastq.gz	Fastqsanger.gz

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Analysis of subcellular transcriptomes by RNA proximity labeling with Halo-seq

Project description:We have developed Halo-seq, an RNA proximity labeling method that allows the quantification of subcellular transcriptomes. We have demonstrated the efficacy of Halo-seq here by using it to quantify chromatin-proximal, nucleolar, and cytoplasmic transcriptomes. In Halo-seq, RNA molecules in close proximity to a spatially restricted protein are specifically marked and biotinylated, facilitating their separation from bulk cellular RNA and their quantification.

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