Project description:Microglia are thought to play a key role in brain in both homeostasis and diseases. The pig has been seen as an attractive, physiologically-relevant model for studying human neurological and mental disorders, as well as being an important agricultural species. Here we sought to characterise microglia markers genes for pigs.
Project description:RATIONALE: Studying the genes expressed in samples of tissue from patients with cancer may help doctors identify biomarkers related to cancer.
PURPOSE: This laboratory study is using gene expression profiling to evaluate normal tissue and tumor tissue from patients with colon cancer that has spread to the liver, lungs, or peritoneum.
Project description:To study the role of LMNA gene in the central nervous system, LMNA gene knock-out mice model and BV2 cell line were obtained. Microglia activation was detected in mice and BV2 cell lines. Phagocytic activity and cytokine production were determined with immunofluorescent and qPCR respectively. Protein profiling of primary microglia and BV2 cells was identified with LC-MS/MS. Differentially expressed proteins were validated by western blotting.
Project description:Microglia represent the resident immune cells of the central nervous system. However, blood-borne monocytic cells are also able to invade the ischemic brain. In this study, we aim to characterize similarities and differences between these two cell populations using gene expression profiling.