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Transcriptomic data from lymphocytic choriomeningitis virus-infected and mock-infected A549 cells

ABSTRACT: Transcriptomic data from lymphocytic choriomeningitis virus-infected and mock-infected A549 cells

PROVIDER: PRJNA723742 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14297316_1.fastq.gz	Fastqsanger.gz
	SRR14297316_2.fastq.gz	Fastqsanger.gz
	SRR14297317_1.fastq.gz	Fastqsanger.gz
	SRR14297317_2.fastq.gz	Fastqsanger.gz
	SRR14297318_1.fastq.gz	Fastqsanger.gz

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Transcriptomic data from lymphocytic choriomeningitis virus-infected and mock-infected A549 cells

Project description:We reported the transcriptomic analysis on A549 cells infected with lymphocytic choriomeningitis virus (LCMV).

2021-04-23 | GSE173072 | GEO

Transcription profiling of human A549 epithelial cells infected with polio virus or mock infected to compare Nanostring and Affymetrix technologies

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Expression data from A549 cell line infected with influenza A virus (IAV) and treated with/without Itaconate

Project description:Analysis of gene expression in A549 cells infected with influenza A virus or Mock and treated with Itaconic acid (IA), or Dimethyl itaconate (DI) to investigate the effects itaconate have on transcriptional response in A549 cells. We particularly looked at anti-inflammatory effects of itaconate in inflammation followed by Influenza A virus infection.

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Project description:propose: The goals of this study are viral transcript of host cells infected with shaan virus and host cell responses in HEK293, A549 and HRT18 infected with shaan virus. Methods: We infected the virus at an MOI of 0.5 to human tissues-derived cell lines including HEK-293, A549 and HRT18. The infected cells were harvested at 24 hours post infection, and a total RNA was extracted from the harvested cells with Trizol LS reagent. The trimmed reads were mapped to shaan virus genome or GRCh37 human genomic DNA reference. The differentially expressed genes (DEGs) between the virus-infected and mock-infected cells were calculated based on the fold changes of FPKM per each gene. Result: Viral transcripts were expressed differentially depending on the viral genes, it is obvious that the nucleocapsid (N) transcript was most expressed in all infected cells. Gene ontology enrichment analysis based on the DEGs revealed that HEK-293, A549 and HRT18 cells highly expressed the genes related to the antiviral defence system and innate immune response in response to the shaan virus infection. Conclusion: In this study, we describe transcriptome data from the shaan virus-infected cells. The inferred DEGs and their related biological functions can be further investigated to understand the shaanvirus-host interaction.

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Proteins modulated during persistent lymphocytic choriomeningitis virus infection

Project description:The experimental data indicate that during a persistent infection, lymphocytic choriomeningitis virus (LCMV) may both directly or indirectly modulate the regulatory cellular processes and alter the cellular functions that are not critical for the survival, but are needed for the homeostasis in the organism. Two-dimensional differential in-gel electrophoresis (2D-DIGE) and MALDI-TOF MS/MS analyses were used to determine the cellular proteome response of HeLa cell line to persistent LCMV infection. Quantitative analysis revealed 24 differentially abundant proteins, half of which were up-regulated and the rest down-regulated. Functional categorization showed that LCMV-responsive proteins were mainly involved in metabolism, stress and defense responses. Among identified proteins, significant changes were found for peroxiredoxins, family of antioxidant enzymes. Decreased amount of these antioxidant proteins was accompanied with the elevation of ROS content in infected cells.

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Project description:Eosinophils are terminally differentiated granulocytes that participate in innate immunity against Influenza A Virus (IAV). Here, we investigate transcriptional changes that occur in IAV-infected human A549 aveolar epithelial carcinoma cells with and without direct or indirect exposure to mouse bone marrow-derived eosinophils (BMdEos). Global changes in the transcriptome of A549 cells due to Influenza A virus-infection and/or direct and indirect eosinophil exposure were assessed using ThermoFisher/Affymetrix Clariom™ S Human microarrays.

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Porcine macrophages: ASFV-infected vs. mock-infected

Project description:Transcriptional profiling of porcine macrophages infected with Afraican swine fever virus (ASFV) infected cells and mock infection was conducted in this experiment.

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