Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A massively parallel reporter assay for RNA localization in neurons

ABSTRACT: A massively parallel reporter assay for RNA localization in neurons

PROVIDER: PRJNA723817 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14300253_1.fastq.gz	Fastqsanger.gz
	SRR14300253_2.fastq.gz	Fastqsanger.gz
	SRR14300254_1.fastq.gz	Fastqsanger.gz
	SRR14300254_2.fastq.gz	Fastqsanger.gz
	SRR14300255_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

A massively parallel reporter assay for RNA localization in neurons

Project description:We performed a massively parallel reporter assay for RNA localization in two mouse neuronal cell lines, CAD and Neuro-2a. We transfected a library of around 50,000 different 3'UTR reporters (cloned downstream of GFP) into cells grown on transwell plates and collected soma and neurite fractions separately. RNA was extracted and cDNA synthesized, followed by targeted amplification of the reporter mRNA and Illumina sequencing.

2021-04-23 | GSE173098 | GEO

Massively parallel splicing reporter assay on designed sequence libraries

Project description:Massively parallel splicing reporter assay on designed sequence libraries

| PRJNA545827 | ENA

Massively parallel reporter assay for identification of RNA switches

Project description:Massively parallel reporter assay for identification of RNA switches

| PRJNA1105614 | ENA

Massively Parallel Reporter Assay for pluripotency factors in mESCs

Project description:A massively parallel reporter assay, MPRA, was conducted in mouse embryonic stem cells (mESC). Synthetic cis-regulatory elements comprised of binding sites for pluripotency transcription factors and genomic sequences with comparable binding sites configurations were used in the assay. Transcripts of dsRed were amplified via PCR from the end of the transcript to sequence 3' UTR barcodes.

2019-09-18 | GSE120240 | GEO

Characterization of maturational enhancer activity by massively parallel reporter assay [amplicon-Seq]

Project description:The activity of enhancers with dynamic P300 binding or mutagenized nuclear receptor motifs was assessed by massively parallel reporter assay during CM maturation.

2023-01-19 | GSE196346 | GEO

Quantification of mouse retinal enhancer activity by massively parallel reporter assay

Project description:Quantification of mouse retinal enhancer activity by massively parallel reporter assay

| PRJNA415987 | ENA

Genome-wide enhancer identification by massively parallel reporter assay in Arabidopsis

Project description:Genome-wide enhancer identification by massively parallel reporter assay in Arabidopsis

| PRJNA659905 | ENA

Massively Parallel Reporter Assay of human-specific substitutions in neurodevelopmental enhancers

Project description:Massively Parallel Reporter Assay of human-specific substitutions in neurodevelopmental enhancers

| PRJNA591611 | ENA

Massively Parallel Reporter Assay of human-specific substitutions in neurodevelopmental enhancers

Project description:We performed a Massively Parallel Reporter Assay (MPRA) to screen >30,000 human-specific substitutions in ChIP-seq-identified Human Gain Enhancers (HGEs) and Human Accelerated Regions (HARs), highly conserved non-coding regions that show accelerated sequence evolution in humans. After comparing human and chimpanzee reference alleles, we used a second MPRA to deconvolute individual substitutions within differentially active enhancers from substitutions in the same fragment and from other variants (human segregating variants or chimpanzee-specific variants) to isolate their specific effects on enhancer activity.

2019-11-26 | GSE140983 | GEO

Characterization of maturational enhancer activity by massively parallel reporter assay [amplicon-Seq]

Project description:Characterization of maturational enhancer activity by massively parallel reporter assay [amplicon-Seq]

| PRJNA804494 | ENA