Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Hesperia comma

ABSTRACT: Hesperia comma overview

PROVIDER: PRJNA724778 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	ERR6054630_1.fastq.gz	Fastqsanger.gz
	ERR6054630_2.fastq.gz	Fastqsanger.gz
	ERR6054626_1.fastq.gz	Fastqsanger.gz
	ERR6054626_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Leucania comma

Project description:Leucania comma overview

| PRJNA990903 | ENA

ilHesComm

Project description:Hesperia comma (common branded skipper)

| PRJEB43800 | ENA

HEB and histone mark ChIP-seq in Comma-Dβ mouse mammary epithelial cells

Project description:The goal of this study was to determine the genome wide binding sites of the E-protein transcription factor HEB in mammary epithelial cells, and to delineate the chromatin context of these regions. ChIP-seq for HEB, H3K4Me3, H3K27Ac, and H3K27Me was performed in Comma-Dβ cells in quadruplicate.

2020-05-07 | GSE149967 | GEO

Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties [scRNA-Seq]

Project description:The mammary epithelial tree is a heterogeneous structure that contains two main populations, luminal and basal, with distinct molecular and functional characteristics. The investigation of how this heterogeneity is developed and how it influences breast tumorigenesis has been hampered by the need to perform these studies using animal models due to the lack of relevant, tractable, and fully characterized in vitro models. Comma-1D is an immortalized mouse mammary epithelial cell line that has unique morphogenetic properties particularly its ability to generate cells of luminal and basal lineages. Here, we perform single-cell RNA-seq studies to dissect its heterogeneous nature. Our analysis reveals that Comma-1D cultures consist of two main populations with luminal and basal features and a smaller population with mixed lineage and bipotent characteristics. Additionally, we demonstrate that multiple transcription factors associated with the differentiation of the mammary epithelium in vivo also modulate this process in Comma-1D cultures. Finally, we have used the Comma-1D model to study how the cellular subtype of origin influences the response to cancer-promoting mutations. We found that only cells with luminal molecular features were able to acquire transformed characteristics after an oncogenic HER2 mutant was introduced in their genomes. Overall, the studies present here characterize at a single-cell level the heterogeneity of the Comma-1D cell line and illustrate how the unique properties of Comma-1D cells can be used as an experimental model to study both the differentiation and the transformation processes in vitro.

2022-05-04 | GSE182352 | GEO

RNA-seq of ID4 knockdown Comma-Dβ mammary epithelial cells

Project description:The goal of this study was to determine the genes regulated by ID4 in mammary epithelial cells. ID4 was knocked down by siRNA in the mammary epithelial cell line Comma-Dβ and RNA-seq was performed following 48 hr to determine differentially expressed genes.

2020-05-07 | GSE149966 | GEO

Single-cell dissection of Comma-1D cultures reveals cell populations with unique differentiation and transformation properties [bulk RNA-Seq]

2022-05-04 | GSE182351 | GEO

ilHesComm1

Project description:Hesperia comma (common branded skipper) genome assembly, ilHesComm1

| PRJEB43996 | ENA

ilHesComm

Project description:Hesperia comma (common branded skipper), genomic and transcriptomic data

| PRJEB43783 | ENA

ilHesComm1 alternate haplotype

Project description:Hesperia comma (common branded skipper) genome assembly, ilHesComm1, alternate haplotype

| PRJEB43997 | ENA

Leveraging single cell transcriptomics and custom high throughput functional screening assays to resolve cell type, growth kinetic, and stemness heterogeneity within Comma-1D

Project description:We investigated the heterogeneous cell populations within the Comma-1D cell line through single cell RNA sequencing technologies. The assay sequenced over 5,000 cells composing 8 unique subclusters. Populations detected included luminal, myoepithelial, and fibroblast like cell types.

2021-12-31 | GSE182589 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data