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BRD4 ChIP-Seq of CD8+ T cell subsets during LCMV infection [ChIP-seq_D7]

ABSTRACT: BRD4 ChIP-Seq of CD8+ T cell subsets during LCMV infection [ChIP-seq_D7]

PROVIDER: PRJNA725876 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14347836.fastq.gz	Fastqsanger.gz
	SRR14347837.fastq.gz	Fastqsanger.gz
	SRR14347838.fastq.gz	Fastqsanger.gz

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BRD4 ChIP-Seq of CD8+ T cell subsets during LCMV infection [ChIP-seq_D7]

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Project description:CD4 and CD8 T cells display functional defects during chronic infection such as loss of certain cytokines. Recent studies have suggested that CD4 T cells may actually gain other functions, however. Here, we analyzed gene expression profiles from LCMV-specific CD4 and CD8 T cells throughout the response to either acute LCMV or chronic LCMV infection. This alllowed us to identify CD4-specific changes during chronic infection compared to acute infection but also revealed shared core regulators between CD4 and CD8 T cells. LCMV-specific CD4 and CD8 T cells were isolated 6, 8, 15 and 30 days post infection with LCMV Armstrong or LCMV clone 13. Naïve CD4 and CD8 T cells were also isolated from naïve mice as comparisons. Four replicates of each sample were hybridized. The only exception is LCMV-specific CD4 T cells isolated 6 days post infection with LCMV-Arm where only three replicates were hybridized.

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