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Transcriptomic analysis of E9.5 mouse embryos

ABSTRACT: Transcriptomic analysis of E9.5 mouse embryos

PROVIDER: PRJNA727827 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14458796_1.fastq.gz	Fastqsanger.gz
	SRR14458796_2.fastq.gz	Fastqsanger.gz
	SRR14458797_1.fastq.gz	Fastqsanger.gz
	SRR14458797_2.fastq.gz	Fastqsanger.gz
	SRR14458798_1.fastq.gz	Fastqsanger.gz

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Transcriptomic profiling of E9.5 mouse embryos lacking the lipoprotein receptor SR-B1

Project description:Transcriptomic profiling of E9.5 mouse embryos lacking the lipoprotein receptor SR-B1

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Transcriptomic profiling of E9.5 mouse embryos lacking the lipoprotein receptor SR-B1

Project description:The high-density lipoprotein receptor SR-B1 mediates cellular uptake of several lipid species, including cholesterol and vitamin E. During early development, SR-B1 is located in the maternal-fetal interface, where it facilitates vitamin E transport towards the embryo. Consequently, embryos lacking SR-B1 are vitamin E-deficient, and around half of them fail to close the neural tube and show neural tube defects (NTD). Here, we studied the transcriptomic profile of mouse embryos lacking SR-B1 to identify the molecular determinants of this phenotypic difference. We used RNA-Seq to analyze the expression of mRNA globally in E9.5 wild-type embryos and embryos lacking SR-B1 with or without NTD, in order to compare expression profiles in those groups and to identify putative genes driving phenotypic differences.

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Microarray analysis of Med23 mutation in E9.5 mouse embryos relative to control littermates

Project description:Microarray analysis of Med23 mutation in E9.5 mouse embryos relative to control littermates

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Genome wide RNA-seq analysis of E9.5 Smchd1 wild type and mutant embryos

Project description:We report the deregulation of expression in E9.5 male mouse embryos are that homozygous for a mutant allele of the Smchd1 gene (ie Smchd1MommeD1/MommeD1).

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RNAseq analysis on e9.5 mutant mouse hearts

Project description:RNAseq analysis on e9.5 mutant mouse hearts

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Genome wide RNA-seq analysis of E9.5 Smchd1 wild type and mutant embryos

Project description:We report the deregulation of expression in E9.5 male mouse embryos are that homozygous for a mutant allele of the Smchd1 gene (ie Smchd1MommeD1/MommeD1). RNA-seq analysis of Smchd1+/+ vs Smchd1MommeD1/MommeD1

2013-07-03 | E-GEOD-44665 | biostudies-arrayexpress

DNA methylation analysis by MeDIP-on-chip in mouse E3.5 blastocysts, E6.5 epiblasts and E9.5 whole embryos.

Project description:DNA methylation is extensively reprogrammed during early phases of mammalian development yet individual genomic targets of this process are largely unknown. We optimized MeDIP (Methylated DNA Immunoprecipitation) for low numbers of cells and profiled DNA methylation genome-wide during early development of the mouse embryonic lineage in vivo. We mapped DNA methylation at 3 consecutive stages of early development: E3.5 blastocysts, E6.5 epiblasts and E9.5 whole embryos. MeDIP and Input samples were hybridized to Nimblegen HD2 MM8 promoter deluxe arrays covering 12 kb of all gene promoters. Experiments were performed in duplicates for E3.5 blastocysts and triplicates for E6.5 epibalsts and E9.5 embryos. As a control we also hybridized pooled unamplified MeDIPs from E9.5 to Nimblegen 385K MM8 RefSeq promoter arrays.

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Gene expression analysis of mouse E9.5 presomitic mesoderm wt vs TCre/+;Taf10flox/flox

Project description:Presomitic mesoderm (PSM) were microdissected from E9.5 mouse embryos (WT and TCre/+;Taf10flox/flox). 3 PSM (17-19 somites stage) were pooled per microarray, in triplicates, per genotypes

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Gene expression data from somites of E9.5 mouse embryos

Project description:We used microarrays to identify Pax3 targets during myogenesis in the mouse embryo Mouse embryos were genotyped Pax3GFP/+ or Pax3PAX3-FKHR/GFP and dissected at E9.5 under a fluorescent binocular. Somites were dissected from the interlimb region and the more hypaxial domain separated from the neural tube and the epaxial extremity of the somites. GFP positive cells were then sorted by flow cytometry before RNA extraction and hybridization on Affymetrix microarrays. We also sorted GFP negative cells.

2010-12-01 | E-GEOD-22040 | biostudies-arrayexpress

Transcriptomic analysis in E9.5 heart tubes identify genes dysregulated in Sox7-null heart tubes

Project description:RNA-seq differential gene expression analysis was accomplished in E9.5 pooled(n = approximately 30) microdissected heart tubes from Sox7-null embryos and a wild-type littermates.

2022-03-01 | GSE168543 | GEO

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