Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiling in RagC Q119L murine germinal centre B cells and B220+ lymphoma cells

ABSTRACT: Gene expression profiling in RagC Q119L murine germinal centre B cells and B220+ lymphoma cells

PROVIDER: PRJNA727837 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14368547.fastq.gz	Fastqsanger.gz
	SRR14368548.fastq.gz	Fastqsanger.gz
	SRR14368549.fastq.gz	Fastqsanger.gz
	SRR14368550.fastq.gz	Fastqsanger.gz
	SRR14368551.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 17

Similar Datasets

Gene expression profiling in RagC Q119L germinal centre B cells

Project description:Gene expression by RNAseq in murine RagC Q119L/+ and RagC +/+ germinal centre B cells

2021-07-13 | GSE174003 | GEO

Gene expression profiling in RagC Q119L murine B220+ lymphoma cells

Project description:Gene expression by RNAseq in murine B220+ from VavP-Bcl2/RagC Q119L/+ and VavP-Bcl2/RagC +/+ lymphomas

2021-07-13 | GSE173619 | GEO

Gene expression profiling in RagC T89N germinal center B cells and RagC S74C murine B220+ lymphoma cells

Project description:Gene expression by RNAseq in murine germinal centers induced by SRBC in RagC T89N mice (YRT codes) and RNAseq in murine B220+ from VavVPBcl2/RagC S74C and VavVPBcl2/RagC +/+ lymphomas Follicular Lymphoma (FL) is an incurable B cell malignancy derived from germinal center (GC) B cells. The RRAGC gene, encoding a GTPase that links cellular nutrient sufficiency with the activation of mechanistic target of rapamycin complex 1 (mTORC1) is mutated in 15% of FL patients, but its impact in B cell functions and lymphomas is unexplored. Endogenous expression of Rragc mutant variants in B lymphocytes from novel knock-in strains of mice resulted in a partial insensitivity of mTORC1 to nutrient deprivation. A mild, but not a full-blown activation of the nutrient signaling pathway enhanced B cell activation and the GC response, suppressed apoptosis, and accelerated lymphomagenesis. Moreover, murine RRAGC mutant FL showed selective vulnerability to pharmacological inhibition of mTORC1. This moderate increase in nutrient signaling synergized with paracrine cues from the supportive T cell microenvironment that activate mTORC1 via the PI3K-Akt axis to promote B cell activation. Hence, RRAGC mutations sustained experimental GCs and murine and human FL in the presence of decreased T cell support. Moreover, mutations in RRAGC in human FLs are mutually-exclusive with mutations that result in increased abundance of T helper cells, consistent with functional redundancy between those pathways. Taken together, our results support a model in which activating mutations in the nutrient signaling pathway foster lymphomagenesis by corrupting a nutrient-dependent control of mTORC1-activating paracrine signals from the T cell microenvironment

2019-07-15 | GSE125394 | GEO

Gene expression profiling in RagC T89N germinal center B cells and RagC S74C murine B220+ lymphoma cells

Project description:Gene expression profiling in RagC T89N germinal center B cells and RagC S74C murine B220+ lymphoma cells

| PRJNA516208 | ENA

Gene expression profiling in RagC Q119L germinal centre B cells

Project description:Gene expression profiling in RagC Q119L germinal centre B cells

| PRJNA727841 | ENA

Gene expression profiling in RagC Q119L murine B220+ lymphoma cells

Project description:Gene expression profiling in RagC Q119L murine B220+ lymphoma cells

| PRJNA726407 | ENA

Gene expression profiling in RagC Q119L murine germinal centre B cells and B220+ lymphoma cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-07-13 | GSE174004 | GEO

Gene expression profiling in RagC S74C germinal center B cells

Project description:Gene expression profiling in RagC S74C germinal center B cells

| PRJNA516209 | ENA

Dynamic mitochondrial transcription and translation in B cells control germinal centre entry and lymphomagenesis

Project description:Study of the effects of Tfam deletion on germinal centre B cells

2022-07-19 | GSE208021 | GEO

Gene expression profiling in RagC S74C germinal center B cells

Project description:Gene expression by RNAseq in murine germinal center B cells induced by SRBC in RagC S74C mice Follicular Lymphoma (FL) is an incurable B cell malignancy derived from germinal center (GC) B cells. The RRAGC gene, encoding a GTPase that links cellular nutrient sufficiency with the activation of mechanistic target of rapamycin complex 1 (mTORC1) is mutated in 15% of FL patients, but its impact in B cell functions and lymphomas is unexplored. Endogenous expression of Rragc mutant variants in B lymphocytes from novel knock-in strains of mice resulted in a partial insensitivity of mTORC1 to nutrient deprivation. A mild, but not a full-blown activation of the nutrient signaling pathway enhanced B cell activation and the GC response, suppressed apoptosis, and accelerated lymphomagenesis. Moreover, murine RRAGC mutant FL showed selective vulnerability to pharmacological inhibition of mTORC1. This moderate increase in nutrient signaling synergized with paracrine cues from the supportive T cell microenvironment that activate mTORC1 via the PI3K-Akt axis to promote B cell activation. Hence, RRAGC mutations sustained experimental GCs and murine and human FL in the presence of decreased T cell support. Moreover, mutations in RRAGC in human FLs are mutually-exclusive with mutations that result in increased abundance of T helper cells, consistent with functional redundancy between those pathways. Taken together, our results support a model in which activating mutations in the nutrient signaling pathway foster lymphomagenesis by corrupting a nutrient-dependent control of mTORC1-activating paracrine signals from the T cell microenvironment

2019-07-15 | GSE125393 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data