Ontology highlight
ABSTRACT:
PROVIDER: PRJNA727958 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR14461611_subreads.fastq.gz | Fastqsanger.gz |
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Project description:In response to the issues of low denitrification efficiency and high N₂O emissions in the biological nitrogen removal process of low C/N municipal wastewater, studies typically address these challenges by adding carbon sources. In this study, the addition of microorganisms enhanced the carbon flow and electron transport for nitrate reduction, significantly improving the denitrification performance of low C/N wastewater and reducing N₂O production. Proteomic analysis was employed to explore the mechanisms underlying this effect. The results revealed that the metabolites produced by the added microorganisms, S. oneidensis MR-1 and B. subtilis, including biosurfactants, heme, and cytochromes, altered the intracellular carbon redistribution in P. denitrificans, leading to an increased carbon flow directed towards nitrate reduction, thus enhancing total nitrogen removal efficiency.
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Project description:Chemosynthetic symbioses occur worldwide in marine habitats, but comprehensive physiological studies of chemoautotrophic bacteria thriving on animals are scarce. Stilbonematinae are coated by monocultures of thiotrophic Gammaproteobacteria. As these nematodes migrate through the redox zone, their ectosymbionts experience varying oxygen concentrations. Here, by applying omics, Raman microspectroscopy and stable isotope labeling, we investigated the effect of oxygen on the metabolism of Candidatus Thiosymbion oneisti. Unexpectedly, sulfur oxidation genes were upregulated in anoxic relative to oxic conditions, but carbon fixation genes and incorporation of 13C-labeled bicarbonate were not. Instead, several genes involved in carbon fixation, organic carbon assimilation and polyhydroxyalkanoate (PHA) biosynthesis, as well as nitrogen fixation and urea utilization were upregulated in oxic conditions. Furthermore, in the presence of oxygen, stress-related genes were upregulated together with vitamin biosynthesis genes likely necessary to withstand its deleterious effects, and fewer symbionts were detected to divide. Based on this first global physiological study of an uncultured chemosynthetic ectosymbiont, we propose that, in anoxic sediment, its proliferation is powered by anaerobic sulfur oxidation coupled to denitrification, whereas in upper layers it makes use of aerobic respiration to facilitate assimilation of carbon and nitrogen, and to survive oxidative stress. The ectosymbiont’s versatile metabolism is thus well-adapted to exploiting a highly changeable environment.
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