Project description:Intracellular microbes are actively present in various tumor types in low biomass and play major role in metastasis. Eliminating intracellular microbes on a cellular level with precision remains a challenge for addressing this, we designed a screening pipeline to characterize intracellular microbes and their interaction with host cells. For that we used host and microbial in-vitro model cell line as HeLa and microbial strain as Escherichia coli 25922. To study dynamics of intracellular microbes we used antibiotics (ampicillin, roxithromycin, ciprofloxacin) and chemotherapy drugs (doxorubicin and cisplatin) as external environmental stress conditions. To effectively eliminate intracellular microbes Permutation Orthogonal Arrays (POA) approach was applied to acquire optimal drug combination regimen in dose and sequence of drugs which reduced 90-95% of intracellular microbial load.
2026-03-02 | PXD057447 | Pride
Project description:Effects of environmental microbes in Drosophila melanogaster
| PRJNA981581 | ENA
Project description:Reservoir water microbes driving environmental geochemical cycles
| PRJNA758548 | ENA
Project description:Amplicon of microbes in digestate: Environmental samples
| PRJNA1130846 | ENA
Project description:Reservoir water microbes drive environmental geochemical cycles
Project description:Intestinal microbiota dysbiosis is related to many metabolic diseases in human health. Meanwhile, as an irregular environmental light-dark cycle, short-day (SD) may induce host circadian rhythms disturbances and worsen the risks of gut dysbiosis. Herein, we investigated how LD cycles regulate intestinal metabolism upon the destruction of gut microbes with antibiotic treatments. The transcriptome data indicated that SD have some negative effects on hepatic metabolism, endocrine, digestive, and diseases processes compared with normal light-dark cycle (NLD).The SD induced epithelial and hepatic purine metabolism pathway imbalance in ABX mice, the gut microbes, and their metabolites, all of which could contribute to host metabolism and digestion, endocrine system disorders, and may even cause diseases in the host.