Project description:The specificity of the RNA-CASing process was analysed by Next-Generation Sequencing. Therfor small RNAs were isolated from purified proteins of Escherichia coli and subjected to Illumina sequencing or nanopore sequencing.
Project description:1. Evaluate the diagnostic value of long noncoding RNA (CCAT1) expression by RT-PCR in peripheral blood in colorectal cancer patients versus normal healthy control personal.
2. Evaluate the clinical utility of detecting long noncoding RNA (CCAT1) expression in diagnosis of colorectal cancer patients & its relation to tumor staging.
3. Evaluate the clinical utility of detecting long noncoding RNA (CCAT1) expression in precancerous colorectal diseases.
4. Compare long noncoding RNA (CCAT1) expression with traditional marker; carcinoembryonic antigen (CEA) and Carbohydrate antigen 19-9 (CA19-9) in diagnosis of colorectal cancer.
Project description:Utilising a whole genome array, we have investigated the differential expression of genes across different developmental stages in the life cycle of Agaricus bisporus. Four biological replicates of each developmental stage (culture, casing, compost, fruiting body) were analysed.
Project description:Interventions: lesion tissues vs. adjacent tissues of colorectal cancer patients:nil
Primary outcome(s): RNA
Study Design: Factorial
Project description:Proteins in Nicotiana benthamiana interacted with RNA fragment (or signal sequences) involving small-RNA accumulation selectivity, were trapped and analyzed.