Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Enterococcus faecalis

ABSTRACT: Genomic diversity and ampicillin/ceftriaxone susceptibility of Enterococcus faecalis from infective endocarditis

PROVIDER: PRJNA729754 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14552119_1.fastq.gz	Fastqsanger.gz
	SRR14552119_2.fastq.gz	Fastqsanger.gz
	SRR14552120_1.fastq.gz	Fastqsanger.gz
	SRR14552120_2.fastq.gz	Fastqsanger.gz
	SRR14552121_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 68

Similar Datasets

Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility in Enterococcus faecalis

Project description:Enterococcus faecalis is a commensal bacterium in the gastrointestinal tract (GIT) of humans and other organisms. Outside of the GIT, E. faecalis can cause infections in root canals, wounds, surgical sites, the urinary tract, and on heart valves. Many of these infections can be polymicrobial, and a variety of toxins and nutritional signals influence interactions between E. faecalis and other microbes. Like other bacteria, E. faecalis metabolizes arginine through the arginine deiminase (ADI) pathway, which converts arginine to ornithine and releases ATP, ammonia, and CO2. E. faecalis arginine metabolism also affects virulence of other pathogens, such as Clostridioides difficile and Escherichia coli, during co-culture. E. faecalis may encounter elevated levels of arginine in the GIT or the oral cavity, where arginine is used as an additive in dental therapeutics to disrupt plaque formation and biofilm growth by oral streptococci. However, little is known about how E. faecalis responds to growth in arginine. To address this gap, we used RNAseq and additional in vitro assays to measure changes in growth, gene expression, and biofilm formation in arginine relative to control conditions. Here, we demonstrate that arginine decreases E. faecalis biofilm production and causes widespread differential expression of genes related to metabolism, nutrient transport, quorum sensing, and polysaccharide synthesis. Growth in arginine also increases aggregation of E. faecalis cultures and promotes decreased susceptibility to the antibiotics ampicillin and ceftriaxone. Together, this shows that E. faecalis undergoes global transcriptional changes during growth in arginine and provides a platform for better understanding of how the presence of arginine in the niches colonized by E. faecalis affects the physiology of this organism in addition to the virulence of surrounding microbes.

2024-05-28 | GSE268264 | GEO

Effect of Gliotoxin on Enterococcus faecalis

Project description:Proteomic analysis of the effects of gliotoxin on Enterococcus faecalis.

2023-05-26 | MSV000092038 | MassIVE

Microdiversity of Enterococcus faecalis isolates from infective endocarditis

Project description:Microdiversity of Enterococcus faecalis isolates from infective endocarditis

| PRJEB36907 | ENA

Grad-seq Enterococcus faecalis and faecium

Project description:Grad-seq in Enterococcus faecalis V583 and Enterococcus faecium AUS004.

2022-11-28 | PXD031577 | Pride

Transcriptome analysis of Enterococcus faecalis OG1 delta-EF2638 mutant

Project description:Analysis of changes in gene expression in Enterococcus faecalis OG1 delta-EF2638 mutant compared to wild-type OG1 strain. The deletion mutant has a growth defect when grown with aeration The mutant presented in this study is described and characterized in Vesic, D. and Kristich, C.J. 2012. A Rex-family transcriptional repressor influnces H2O2 accumulation by Enterococcus faecalis. (submitted for publication)

2013-04-15 | GSE43228 | GEO

Enterococcus Faecalis V583 secretome

Project description:Identification of proteins in the secretome of Enterococcus Faecalis V583 after growth on urine

2015-11-03 | PXD001345 | Pride

Transcriptomic response of Enterococcus faecalis to iron excess

Project description:To further investigate the homeostatic response of E. faecalis to Fe exposure, we examine the whole-genome transcriptional response of wild-type (WT) exposed to non toxic Fe excess. This experiment correspond the work titled Transcriptomic response of Enterococcus faecalis to iron excess (work in preparation) A four chip study using total RNA recovered from four separate wild-type cultures of Enterococcus faecalis OG1RF, two controls samples (N medium growth) and two iron samples (N medium gowth with 0.5 mM Fe-NTA). Each chip measures the expression level of 3,114 genome genes from Enterococcus faecalis strain V583 (A7980-00-01).

2012-03-01 | E-GEOD-34432 | biostudies-arrayexpress

Transcriptional Profiling of Enterococcus faecalis During Surface Penetration

Project description:To investigate the transcriptional changes that Enterococcus faecalis undergoes during agar surface-penetration, which promote cell envelope remodeling and tolerance to stress.

2022-10-19 | GSE212145 | GEO

Transcriptomic response of Enterococcus faecalis to iron excess

2012-03-01 | GSE34432 | GEO

Bacterial bunker blueprints: IreK-mediated, cell wall protective phosphorylation in Enterococcus faecalis

Project description:Enterococcus faecalis is a Gram-positive bacterium that is a major cause of hospital-acquired infections due to its intrinsic resistance to cell wall-active antimicrobials. One critical determinant of this resistance is the transmembrane kinase IreK, which belongs to the PASTA kinase family of bacterial signaling proteins involved with the regulation of cell wall homeostasis. IreK has enhanced activity in response to cell wall stress, but direct substrates of IreK phosphorylation leading to antimicrobial resistance are largely unknown. To better understand stress-modulated phosphorylation events contributing to virulence, wild type E. faecalis treated with cell wall-active chlorhexidine and ceftriaxone were examined via phosphoproteomics. Among the most prominent changes were increased phosphorylation of divisome components after both treatments, implicating cell division proteins in antimicrobial defense signaling. Phosphorylation mediated by IreK was then determined via similar analysis with a E. faecalis ΔireK mutant strain, revealing potential IreK substrates involved with the formation/maintenance of biofilms and within the E. faecalis two-component system, another common signal transduction pathway for antimicrobial resistance. These results reveal critical insights into the biological functions of IreK and the mechanisms of E. faecalis antimicrobial resistance.

2021-11-03 | PXD023265 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data