Transcriptome and oxylipin profiling joint analysis reveals the opposite roles of 9-oxylipins and jasmonic acid in maize resistance to Gibberella stalk rot
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA730310 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR14559703_1.fastq.gz | Fastqsanger.gz | |||
| SRR14559703_2.fastq.gz | Fastqsanger.gz | |||
| SRR14559704_1.fastq.gz | Fastqsanger.gz | |||
| SRR14559704_2.fastq.gz | Fastqsanger.gz | |||
| SRR14559705_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 36 |
Similar Datasets
Project description:Gibberella stalk rot (GSR) caused by Fusarium graminearum is one of the devastating diseases causing significant losses to maize production worldwide. Although plant oxylipins have been widely reported as potent signals to activate diverse biotic stress responses, the roles of distinct oxylipin pathway branches initiated by either 9- or 13-lipoxygenases (LOXs) in defense against GSR remain unexplored. In this study, the functional analysis showed that disruption of ZmLOX5, a maize 9-LOX gene, resulted in increased susceptibility to GSR. To identify the key genes and metabolites associated with GSR resistance, we profiled transcriptome and oxylipins in the lox5 mutant and near-isogenic wild type. The results showed that JA biosynthetic pathway genes are highly up-regulated, whereas multiple 9-LOX pathway genes down-regulated in lox5-3 mutant in response to F. graminearum infection. Furthermore, oxylipin profiling of the mutant and corresponding wild type, B73, as well as a more resistant line, W438, uncovered significantly higher contents of JA-isoleucine (JA-Ile) and other jasmonates but relatively lower levels of 9-oxylipins in lox5-3 upon infection. By contrast, resistant line W438 and B73 displayed relatively lower levels of JAs, yet considerable increase of 9-oxylipins. Taken together, these results clearly indicated that the signaling pathways of 9-oxylipins and JAs antagonize each other, and that while ZmLOX5-produced 9-oxylipins contribute to resistance, JAs are likely to function as negative regulator in maize defense against GSR.
2021-05-17 | GSE174508 | GEO
Project description:Gibberella stalk rot caused by Fusarium graminearum is one of the devastating diseases of maize that causes significant yield losses worldwide. The molecular mechanisms regulating defense against this pathogen remain poorly understood. According to recent studies, a major oxylipin hormone produced by 13-lipoxygenases (LOX) namely jasmonic acid (JA) has been associated with maize susceptibility to GSR. However, the specific roles of numerous 9-LOX-derived oxylipins in defense against Gibberella stalk rot (GSR) remain unexplained. In this study, we have shown that disruption of a 9-LOX gene, ZmLOX5, resulted in increased susceptibility to GSR, indicating its role in defense. To understand how ZmLOX5 regulates GSR resistance, we conducted transcriptome and oxylipin profiling using a zmlox5-3 mutant and near-isogenic wild type B73, upon infection with F. graminearum. The results showed that JA biosynthetic pathway genes were highly up-regulated, whereas multiple 9-LOX pathway genes were down-regulated in the infected zmlox5-3 mutant. Furthermore, oxylipin profiling of the mutant revealed significantly higher contents of several jasmonates but relatively lower levels of 9-oxylipins in zmlox5-3 upon infection. In contrast, B73 and W438, a more resistant inbred line, displayed relatively lower levels of JAs, but a considerable increase of 9-oxylipins. These results suggest antagonistic interaction between 9-oxylipins and JAs, wherein 9-oxylipins contribute to resistance while JAs facilitate susceptibility to F. graminearum.
| S-EPMC8454893 | biostudies-literature
Project description:12-oxo-phytodienoic acid (OPDA) and phytoprostane A1 (PPA1) are cyclopentenone oxylipins that are formed via the enzymatic jasmonate pathway and a nonenzymatic, free radical–catalyzed pathway, respectively. Both types of cyclopentenone oxylipins induce the expression of genes related to detoxification, stress responses, and secondary metabolism, a profile clearly distinct from that of the cyclopentanone jasmonic acid. To investigate the role of TGA transcriction factors in oxylipin responses, the regulation of gene expression by OPDA and PPA1 defective in the expression of TGA2, TGA5, and TGA6 was analyzed using Affymetrix ATH1 chips. Keywords: Arabidopsis tga2-5-6 mutant, Oxylipin treatment, ATH1 Chip
2008-06-02 | GSE10732 | GEO
Project description:Reproducibility issues regarding complex in vitro cell culture experiments have been mainly attributed to genetic variations within cell lines. Batch-dependent fetal calf serum variations are well known and also represent relevant influencing factors. However, the molecular constituents mediating such effects remained largely unknown. High resolution mass spectrometry is a powerful tool to identify molecular FCS constituents and investigate their effect on cultured cells. Using a differentiation and inflammatory stimulation protocol on U937 cells we observed FCS batch-dependent variations of secreted cytokines, oxylipins and other inflammatory mediators. In order to detect candidate bioactive molecules contained in FCS, the protein and oxylipin composition of FCS batches was investigated. Remarkably, the protein composition showed some, but much less batch-dependent variation when compared to the oxylipin composition. Efficient uptake of C13-labelled arachidonic acid from medium by U937 macrophages and inflammation-induced release thereof including oxylipin products was evidenced. Balancing out FCS batch-dependent nanomolar concentration differences of two selected oxylipins, 5-HETE and 15-HETE, by spiking experiments, resulted in significant proteome alterations of cultured U937 macrophages indicating PPAR activation. High-resolution microscopy demonstrated HETE-induced formation of peroxisomes, thus independently corroborating the proteome profiling results. In conclusion, the present data demonstrate a strong and previously underappreciated influence of FCS-contained oxylipins on cellular effector functions, thus representing a plausible cause for disturbing reproducibility issues.
2021-06-01 | PXD020617 | Pride
Project description:Oxygenated unsaturated fatty acids, known as oxylipins, are signaling molecules commonly used for cell-to-cell communication in eukaryotes. However, a role for oxylipins in mediating communication in prokaryotes has not previously been described. Bacteria mainly communicate via quorum sensing (QS) , which involves the production and detection of diverse small molecules termed autoinducers. We showed that oleic acid-derived oxylipins 10-HOME and 7,10-DiHOME produced by Pseudomonas aeruginosa function as autoinducers of a novel quorum sensing system termed Oxylipin-Dependent QS Sytem (ODS). This experiment was designed to determine the genes whose expression is altered by these P. aeruginosa oxylipins. We found that the ODS system controls the cell density-dependent expression of a P. aeruginosa gene subset through the mediation of 10-HOME and 7,10-DiHOME oxylipins.
2018-12-05 | GSE123356 | GEO
Project description:12-oxo-phytodienoic acid (OPDA) and phytoprostane A1 (PPA1) are cyclopentenone oxylipins that are formed via the enzymatic; jasmonate pathway and a nonenzymatic, free radicalâcatalyzed pathway, respectively. Both types of cyclopentenone oxylipins; induce the expression of genes related to detoxification, stress responses, and secondary metabolism, a profile clearly distinct; from that of the cyclopentanone jasmonic acid. To investigate the role of TGA transcriction factors in oxylipin responses, the regulation of gene expression by OPDA and PPA1 defective in; the expression of TGA2, TGA5, and TGA6 was analyzed using; Affymetrix ATH1 chips. Experiment Overall Design: 18 hybs total
2008-06-18 | E-GEOD-10732 | biostudies-arrayexpress
Project description:Transcriptome analysis of maize Gibberella stalk rot
| PRJNA721468 | ENA
Project description:Gibberella stalk rot (GSR) caused by Fusarium graminearum is one of the most devastating diseases causing significant yield loss of maize, and GSR resistance is a quantitative trait controlled by multiple genes. Although a few QTLs/resistance genes have been identified, the molecular mechanisms underlying GSR resistance remain largely unexplored. To identify potential resistance genes and to better understand the molecular mechanism of GSR resistance, a transcriptomic was conducted using two inbred lines with contrast GSR resistance, K09 (resistant) and A08 (susceptible) upon infection with F. graminearum. While substantial number of differentially expressed genes (DEGs) associated with various defense-related signaling pathways were identified between two lines, multiple hub genes likely associated with GSR resistance were pinpointed using Weighted Gene Correlation Network Analysis (WGCNA). ZmHIR3 showed strong correlation with multiple key genes.
2021-04-13 | GSE171968 | GEO
Project description:Fusarium graminearum is the causal agent of Gibberella stalk rot in maize stem, resulting maize lodging, yield, quality, and mechanical harvesting capacity. To date, little is known about the maize stem defense mechanism in response to invasion of F. graminearum. This study represents a global proteomic approach to document the infection by F. graminearum. A total of 1,894 differentially accumulated proteins (DEPs) were identified after inoculation of maize plants with F. graminearum. Functional categorization analysis indicated that proteins involved in plant-pathogen interaction were inducible at early stages of infection. We also found that the expression of proteins involved in phenylpropanoid, flavonoid, and terpenoid biosynthesis were up-regulated in response to F. graminearum infection, which may reflect a particular contribution of secondary metabolism in protection against the fungal attack in maize stem. Together, our results indicated that the defense response of maize stem to F. graminearum infection was multifaceted and involved the induction of proteins from various innate immunity related pathways, which had directive significance for molecular genetic breeding of maize disease-resistant varieties.
2021-05-31 | PXD024342 | Pride
Project description:Pyroptosis is a lytic cell death mode that helps limit the spread of infections and is also linked to pathology in sterile inflammatory diseases and autoimmune diseases. During pyroptosis, inflammasome activation and the engagement of caspase-1 lead to cell death, along with the maturation and secretion of the inflammatory cytokine interleukin 1β (IL-1β). The dominant effect of IL-1β in promoting tissue inflammation has clouded the potential influence of other factors released from pyroptotic cells. Here, using a system where macrophages are induced to undergo pyroptosis without IL-1β/⍺ release (denoted Pyro-1), we uncover unexpected beneficial effects of the Pyro-1 secretome. First, we noted that the Pyro-1 supernatants upregulated gene signatures linked to migration, cellular proliferation, and wound healing. Consistent with this gene signature, Pyro-1 supernatants boosted migration of primary fibroblasts and macrophages, as well as faster wound closure in vitro, and improved tissue repair in vivo. In mechanistic studies, lipidomics and metabolomics of the Pyro-1 supernatants identified the presence of both oxylipins and metabolites, linking them to pro-wound healing effects. Focusing specifically on the oxylipin prostaglandin E2 (PGE2), we find that PGE2 synthesis is induced de novo during pyroptosis, downstream of caspase-1 activation, and COX2 activity; further, PGE2 synthesis occurs late in pyroptosis, with its release dependent on Gasdermin D pores opened during pyroptosis. As for the pyroptotic metabolites, they link to immune cell infiltration into the wounds, and polarization to CD301+ macrophages. Collectively, these data advance the concept that the pyroptotic secretome 1 Mehrotra et al., Page No. possesses oxylipins and metabolites with tissue repair properties that may be harnessed therapeutically.
2024-04-07 | GSE262847 | GEO