Identification of Differentially Expressed Genes in Human Gingival and Periodontal Derived Stromal Cells Compared with Human Dermal Fibroblasts
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ABSTRACT:
PROVIDER: PRJNA730697 | ENA |
REPOSITORIES: ENA
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Project description:The normal epithelium derived stromal cells included ECM, normal fibroblast, mesenchymal stromal cells and osteoblast also plays a significant role in the progress of cancers but poorly investigated in the progress of OSCC. In this study, the stromal cells derived from gingival and periodontal tissues were extracted and human dermal fibroblasts were selected as control group. The differentially expressed genes in Gingival derived stromal cells and Periodontal derived stromal cells were identified by microarray. Furthermore, the biological process, cellular component, molecular function and cell pathways of differentially expressed genes in gingival derived stromal cells and periodontal derived stromal cells were analyzed by GO enrichment analysis and KEGG enrichment analysis respectively. All of these findings could provide potential genes in gingival derived stromal cells and periodontal derived stromal cells that influences the progress of OSCC.
2022-08-23 | GSE174595 | GEO
Project description:We investigated differential DNA methylation in gingival tissues in states of periodontal health, gingivitis and periodontitis.
2021-04-23 | GSE173081 | GEO
Project description:We investigated differential mRNA expression in gingival tissues in states of periodontal health, gingivitis and periodontitis.
2021-04-23 | GSE173078 | GEO
Project description:Fibroblasts are widely used cells for regenerative medicine in clinics, such as gingival or facial skin treatment. In fact, fibroblasts are considered as a mixture of various types of cells with "spindle shape" and there is no available clear marker. Gingival and dermal fibroblasts are similar in their morphology and function; however it is considered that the cultured cells retain their original characteristics depending on the origin, which may contribute to the differential therapeutic effects. For example, gingival wounds are known to heal relatively quickly with less scar formation compared with skin, which may imply their higher capability for regeneration as a therapeutic effect. The reason for this phenomenon may be partly due to characteristic differences between gingival and dermal fibroblasts including the expression of migration stimulating factor and matrix formation but these differences remain largely unknown. Recently, the characteristics of dermal fibroblasts have been reported to be different depending on body sites, such as face, trunk and plamoplantar skin. Although the expression of fibronectin and their splicing variants were known to be different between trunk and oral mucosal fibroblasts, there is still no detailed report on the functional differences between gingival and dermal fibroblasts. In this study, we investigated differential gene expression in normal gingival and dermal fibroblasts using DNA microarray to investigate the difference between the vague fibroblast-type cells from different tissue origin to achieve higher therapeutic effect in cell therapy.
2010-05-28 | GSE22029 | GEO
Project description:Fibroblasts are widely used cells for regenerative medicine in clinics, such as gingival or facial skin treatment. In fact, fibroblasts are considered as a mixture of various types of cells with "spindle shape" and there is no available clear marker. Gingival and dermal fibroblasts are similar in their morphology and function; however it is considered that the cultured cells retain their original characteristics depending on the origin, which may contribute to the differential therapeutic effects. For example, gingival wounds are known to heal relatively quickly with less scar formation compared with skin, which may imply their higher capability for regeneration as a therapeutic effect. The reason for this phenomenon may be partly due to characteristic differences between gingival and dermal fibroblasts including the expression of migration stimulating factor and matrix formation but these differences remain largely unknown. Recently, the characteristics of dermal fibroblasts have been reported to be different depending on body sites, such as face, trunk and plamoplantar skin. Although the expression of fibronectin and their splicing variants were known to be different between trunk and oral mucosal fibroblasts, there is still no detailed report on the functional differences between gingival and dermal fibroblasts. In this study, we investigated differential gene expression in normal gingival and dermal fibroblasts using DNA microarray to investigate the difference between the vague fibroblast-type cells from different tissue origin to achieve higher therapeutic effect in cell therapy. Gingival and dermal tissues were collected from healthy patients. After successive stages primary culture and RNA extraction and hybridization on Affymetrix microarrays (Genome Focus Array). Dermal and gingival tissues were obtained from healthy volunteers (8 cases for dermal tissue; 6 females, 2 males , average age 48 and 8 cases for buccal gingival tissue; 6 females, 2 males, average age 43) whose informed consent was obtained according to a protocol approved by the ethics committee of Nagoya University Hospital. After enzymatic digestion, tissues were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum at 37°C in the presence of 5% CO2 for about 4 weeks as reported previously. Total mRNAs were extracted from cells between passages 4-5 by Trizol reagent (Invitrogen, Carlsbad, CA, USA) and were applied to Human Focus Arrays (Affymetrix, Santa Clara, CA, USA) for microarray analysis according to the manufacturer's protocol (http://www.affymetrix.com/support/technical/manuals.affx). The gene expression data were analyzed by Arrayassist (Stratagene, La Jolla, CA, USA). Briefly, 8,500 probes on the array, normalization and scaling (MAS5), flag-positive gene selection, unpaired t-test, and CV selection (<20) resulted in 5,284 genes to analyze. GO (Gene ontology) analysis was performed using the software default settings to find the gene group related to the same category of biological function by searching common key terms that were reported for each gene.
2010-06-08 | E-GEOD-22029 | biostudies-arrayexpress
Project description:Cementum protein 1 (CEMP1) is involved in cell adhesion, cell differentiation, regulates the mineralization processes during cementogenesis, and selects progenitor cells from periodontal ligament. To determine the gene expression profiling induced by CEMP1 overexpression by non-osteogenic cells such as human gingival fibroblasts we performed microarrays (Human Gene 1.0 ST, Affymetrix) to identify differentially expressed transcripts (ED) between human gingival fibroblasts (HGF) and those that overexpress CEMP1 (HGF/CEMP1).
2014-09-16 | GSE53929 | GEO
Project description:The change of transcriptome and related biological functions affected by tobacco smoke and Electronic-cigarette (E-cig) to human gingival mesenchymal (GMSC) and periodontal ligament stem cells (PDLSCs) are not fully understood. Here, we collected GMSCs and PDLSCs from systemically healthy patients due to the premolars and extracted for orthodontic reasons, and treated with tobacco smoke (G1, P1), E-cig smoke with original flavor (G2, P2), E-cig smoke with menthol flavor (G3, P3), E-cig liquid with original flavor (G4, P4), and E-cig liquid with menthol flavor (G5, P5) by the optimal conditions of LC50, and conducted RNA-seq to compare with untreated control (G0, P0).
2022-06-17 | E-MTAB-11797 | biostudies-arrayexpress
Project description:We examined gene expression signatures in healthy and diseased gingival tissues in 90 patients. Analysis of the gingival tissue transcriptome in states of periodontal health and disease may reveal novel insights of the pathobiology of periodontitis. Keywords: gingival tissue disease state analysis
2008-12-22 | GSE10334 | GEO
Project description:Periodontal regeneration study. Periodontitis is a common chronic inflammatory disease which may lead to tooth loss. The ultimate goal of periodontal therapy is complete regeneration of the tissues lost as a result of periodontitis. However, most regenerative clinical procedures are unpredictable, largely because there is a lack of understanding of how the various tissues comprising the periodontium (gingival epithelium, gingival connective tissue, periodontal ligament, aveolar bone and cementum) interact during the regenerative process. The aim of this study was to investigate the molecular mechanisms that are involved in periodontal regeneration. For this purpose, paired sets of gingival, ligament and regenerating cells were isolated from patients that have been treated for periodontitis via a regenerative surgical procedure. A microarray analysis using the Hu133A Affymetrix arrays was used to identify the genes and pathways that were characteristic of the three different tissues. We have identified over 500 transcripts in global comparison and intrapatient comparisons that were differentially expressed between ligament and gingival samples, and approximately 30 transcripts that characterized the regenerating cell population. By using a functional classification based on Gene Ontology we were able to group the transcripts into 12 groups. Proteases/Protease inhibitors were differentially expressed between the ligament and gingiva highlighting high ECM remodelling activity by gingival cells. On the other hand, ligament cells were shown to have increased protein synthesis by increasing their nucleolar and ribosomal gene complement. We were able to identify 57 various DNA binding proteins, among them were some well characterised transcription factors, transcriptional regulators, including DNA polymerases and transcriptional co-activators. Furthermore, we have also identified changes in expression of their targets in the two tissues. This is the first study to characterise the gene expression profile of periodontal regenerating cells and allowed us to gain valuable insights into the cellular and molecular mechanisms that occur during the regenerative process. We have identified signalling pathways which are consistent with clinical observations of the regenerative properties of gingival and periodontal ligament cells. These pathways provide candidate targets that can be manipulated in order to achieve a superior regenerative clinical outcome. Keywords: other
2006-04-13 | GSE2525 | GEO
Project description:Cementum protein 1 (CEMP1) is involved in cell adhesion, cell differentiation, regulates the mineralization processes during cementogenesis, and selects progenitor cells from periodontal ligament. To determine the gene expression profiling induced by CEMP1 overexpression by non-osteogenic cells such as human gingival fibroblasts we performed microarrays (Human Gene 1.0 ST, Affymetrix) to identify differentially expressed transcripts (ED) between human gingival fibroblasts (HGF) and those that overexpress CEMP1 (HGF/CEMP1). HGF cells overexpressing CEMP1 and controls were harvested at 3, 7 and 14 days after transfection of CEMP1, three biological replicates were analyzed for each time point and condition for a total of 18 microarrays.
2014-09-16 | E-GEOD-53929 | biostudies-arrayexpress