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ABSTRACT: Effect of 12-HETE treatment of cardiomyocytes after hypoxia-reoxygenation challenge

PROVIDER: PRJNA732135 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14627566.fastq.gz	Fastqsanger.gz
	SRR14627567.fastq.gz	Fastqsanger.gz
	SRR14627568.fastq.gz	Fastqsanger.gz
	SRR14627569.fastq.gz	Fastqsanger.gz
	SRR14627570.fastq.gz	Fastqsanger.gz

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Project description:RNA sequencing and subsequent bioinformatics analyses were performed at early reoxygenation stages in HL-1 cardiomyocytes treated or not with BRL37344 Methods: HL-1 cardiomyocytes were subjected to Hypoxia/Reoxygenation (6h/1h) with/without a M-NM-23AR agonist (BRL37344 5M-BM-5mol/L). mRNA profiles were generated by deep sequencing, in triplicate, using Illumina GAIIx.The sequence reads that passed quality filters were quantified using BWA aligned reads using RSEM. qRTM-bM-^@M-^SPCR validation was performed using SYBR Green assay. Results: After 6h of hypoxia followed by 1h reoxygenation, 866 genes were differentially expressed upon M-NM-23AR stimulation by BRL37344. Among these, 177 were at least 2-fold up or downregulated. Conclusions: Our results show that Hsp70 plays a key role in the cardioprotection afforded by M-NM-23AR agonism in cardiomyocytes during the early window of H/R. mRNA profiles from cardiomyocyte subjected to H/R (6h/1h) with/without a M-NM-23AR agonist were generated by deep sequencing, in triplicate, using Illumina GAIIx.

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Project description:RNA sequencing and subsequent bioinformatics analyses were performed at early reoxygenation stages in HL-1 cardiomyocytes treated or not with BRL37344 Methods: HL-1 cardiomyocytes were subjected to Hypoxia/Reoxygenation (6h/1h) with/without a β3AR agonist (BRL37344 5µmol/L). mRNA profiles were generated by deep sequencing, in triplicate, using Illumina GAIIx.The sequence reads that passed quality filters were quantified using BWA aligned reads using RSEM. qRT–PCR validation was performed using SYBR Green assay. Results: After 6h of hypoxia followed by 1h reoxygenation, 866 genes were differentially expressed upon β3AR stimulation by BRL37344. Among these, 177 were at least 2-fold up or downregulated. Conclusions: Our results show that Hsp70 plays a key role in the cardioprotection afforded by β3AR agonism in cardiomyocytes during the early window of H/R.

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Project description:Acute myocardial infarction is a leading cause of death among adults globally. Hypoxia/reoxygenation (H/R) injury plays a causal role in myocardial damage and death, whose molecular mechanisms remain largely elusive. Our present work employed rat H9C2 cardiomyocytes to establish an H/R injury model and investigated differential expression profiles following H/R treatment using RNA-seq. Normal cultured H9C2 cardiomyocytes were used as the control group.

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Down-regulation of NDRG1 promotes migration of cancer cells during reoxygenation

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