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Single Cell RNA-Seq of the embryonic day 14.5 genital tubercle

ABSTRACT: Single Cell RNA-Seq of the embryonic day 14.5 genital tubercle

PROVIDER: PRJNA732639 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14639442.fastq.gz	Fastqsanger.gz
	SRR14639443.fastq.gz	Fastqsanger.gz

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Single Cell RNA-Seq of the embryonic day 14.5 genital tubercle

Project description:Characterize the cellular diversity of the embryonic genital tubercle before sexual dimorphic morphogenesis in male and female mice.

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Gene expression analysis of male and female embryonic day 14 genital tubercle (GUDMAP Series ID: 45).

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus Microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 genital tubercle of the male and the female. The genital tubercle region from E14 (TS22) FVB/N males and females were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

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Gene expression analysis of male and female embryonic day 14 genital tubercle (GUDMAP Series ID: 45).

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Transcription profiling of mouse embryonic day 14 gential tubercle of the male. (GUDMAP Series_id: 16)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 gential tubercle of the male. Experiment Overall Design: The genital tubercle region from an E14 (TS22) SMAA/EYFP males were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

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RNA-seq of mouse barrelette neurons at embryonic day 14.5

Project description:RNA-seq of mouse barrelette neurons at embryonic day 14.5

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Gene expression analysis of male E14 genital tubercle isolated from SMAA (Acta2) transgenic mice. (GUDMAP Series ID: 16)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 gential tubercle of the male. Keywords: Gene expression comparison from developing regions of the embryonic urogenital system.

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