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Single Cell RNA-Seq of the embryonic day 14.5 genital tubercle

ABSTRACT: Single Cell RNA-Seq of the embryonic day 14.5 genital tubercle

PROVIDER: PRJNA732639 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14639442.fastq.gz	Fastqsanger.gz
	SRR14639443.fastq.gz	Fastqsanger.gz

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Single Cell RNA-Seq of the embryonic day 14.5 genital tubercle

Project description:Characterize the cellular diversity of the embryonic genital tubercle before sexual dimorphic morphogenesis in male and female mice.

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Gene expression analysis of male and female embryonic day 14 genital tubercle (GUDMAP Series ID: 45).

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus Microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 genital tubercle of the male and the female. The genital tubercle region from E14 (TS22) FVB/N males and females were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

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Gene expression analysis of male and female embryonic day 14 genital tubercle (GUDMAP Series ID: 45).

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Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 gential tubercle of the male. Experiment Overall Design: The genital tubercle region from an E14 (TS22) SMAA/EYFP males were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

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RNA-seq of mouse barrelette neurons at embryonic day 14.5

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Project description:Despite the incredible morphological diversity found within vertebrates, embryonic development is often regulated by shared foundational mechanism maintained from a common ancestor. For instance, the tetrapod limb displays tremendous variation in size and shape but largely develops according to conserved gene regulatory networks, including signaling pathways, transcription factors, and enhancers. The phallus of amniotes is also thought to share a basic homology originating from their last common ancestor over 300 million years ago. The genital tubercle (GT) of mammals – the embryonic precursor to the penis and clitoris – shows similarities with the external genitalia of other amniotes prior to sexual differentiation. Many genes and enhancers show a common role in both the limbs and the external genitalia, and disruption of these loci can cause aberrant phenotypes in both structures. Among these shared limb-genital genes is Isl1. The Isl1 gene encodes a homeodomain transcription factor that is required for initiation of the hindlimb bud and for normal GT development. This study uses a combination of RNA-seq, ChIP-seq, and comparative genomics to identify potential targets of ISL1 during both hindlimb initiation and GT outgrowth. ChIP-seq data from mouse, chick, and other species reveals targets that are likely conserved from the last common ancestor of amniotes. A subset of ISL1 targets are shared between the hindlimb and the external genitalia, supporting the hypothesis that aspects of phallus development were coopted from the limb gene regulatory network. This systematic investigation of ISL1 transcriptional targets expands our knowledge of Isl1’s role in appendage development and generates further testable hypotheses concerning the establishment of these structures.

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Downstream targets of Isl1 in the hindlimb field and genital tubercle

Project description:Downstream targets of Isl1 in the hindlimb field and genital tubercle

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Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 gential tubercle of the male. Keywords: Gene expression comparison from developing regions of the embryonic urogenital system.

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