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An amyloid protein and the lipopeptide fengycin are involved in the communication and beneficial interaction of B. subtilis with seeds

ABSTRACT: An amyloid protein and the lipopeptide fengycin are involved in the communication and beneficial interaction of B. subtilis with seeds

PROVIDER: PRJNA733070 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14664476_1.fastq.gz	Fastqsanger.gz
	SRR14664476_2.fastq.gz	Fastqsanger.gz
	SRR14664477_1.fastq.gz	Fastqsanger.gz
	SRR14664477_2.fastq.gz	Fastqsanger.gz
	SRR14664478_1.fastq.gz	Fastqsanger.gz

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An amyloid protein and the lipopeptide fengycin are involved in the communication and beneficial interaction of B. subtilis with seeds

Project description:We report the application of a high-throughput technique, RNA-seq, to study the transcriptomic response of cucumis melo seeds and leaves to the treatment with B. subtilis cells or the secondary metabolite fengycin

2022-04-20 | GSE175611 | GEO

Synthetic self-adjuvanted lipopeptide vaccines conferred protection against Helicobacter pylori infection

Project description:Helicobacter pylori (H. pylori) colonize the stomach epithelium of half the world's population and are responsible for a variety of digestive diseases and even stomach cancer. The protection against H. pylori infection depends primarily on the antigen-mediated mucosal and T cell responses. We hypothesized that lipopeptide vaccines obtained by covalently conjugation of Pam2Cys with strong mucosal adjuvant activity to the immunodominant epitope from protective antigens could induce protective responses against H. pylori infection. In this study, the synthetic lipopeptide vaccines, Hp4 (Pam2Cys modified UreB T cell epitope) and Hp10 (Pam2Cys modified CagA T/B cell combined epitope), induced the BMDCs maturation in vitro by activating a variety of pattern recognition receptors such as TLR, NLR and RLR. In addition, lipopeptide vaccines stimulated BMDCs to secret cytokines that have the potential to modulate T cell activation and differentiation. Although intranasal immunization with Hp4 or Hp10 elicited robust epitope-specific T cell responses in mice, only Hp10 conferred protection against H. pylori infection, possibly due to the fact that Hp10 also induced substantial specific sIgA response at mucosal sites. Interestingly, when two lipopeptide vaccines were administrated in combination, Hp4 elevated the protective response against H. pylori infection of Hp10, which was characterized by better protective effect and enhanced specific T cell and mucosal antibody responses. Our results suggest that synthetic lipopeptide vaccines based on the epitopes derived from the protective antigens are promising candidates for protection against H. pylori infection.

2023-01-01 | GSE218459 | GEO

Deciphering immune responses primed by a bacterial lipopeptide in wheat towards Zymoseptoria tritici

Project description:We revealed that mycosubtilin, a lipopeptide from Bacillus subtilis, protects wheat against the hemibiotrophic fungal pathogen Zymoseptoria tritici. Foliar application of the biomolecule primes, during the early stages of infection, the expression of genes associated with sixteen functional groups, including responses to pathogens, abiotic and oxidative stresses, secondary metabolism, cell-wall structure and function, and primary metabolic pathways (carbohydrate, amino acid, protein, lipid, and energy metabolisms).

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Identify gene networks in maize and beneficial fungus Trichoderma virens that result in induced systemic resistance (ISR)

Project description:ISR is the initiation of a beneficial association by certain fungi in the rhizosphere followed by the establishment of belowground-aboveground signaling communication may result in the induction of heightened host resistance to foliar and stem pathogens, as well as insect pests.

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A novel model of Amyloid-beta pathology in C. elegans reveals critically vulnerable neurons as seeds of pathogenicity

Project description:A novel model of amyloid-beta pathology in C. elegans has been generated which allows for substoichiometric fluorescent labeling of amyloid-beta species in living nematodes. By microscopy, FLIM, physiological and biochemical studies, the progress of neurodegeneration and general pathogenicity of amyloidogenic peptides is confirmed. Notably, a single set of neurons was shown to be most vulnerable to Amyloid-beta overexpression and putatively act as a seed for systemic pathogeny. A neuronal version of the amyloid-beta reporter strain was used to perform proteomics analysis from worm lysates after enrichment by immunoprecipitation, using anti-amyloid-beta antibodies.

2020-09-18 | PXD017591 | Pride

A novel model of Amyloid-beta pathology in C. elegans reveals critically vulnerable neurons as seeds of pathogenicity

Project description:A novel model of amyloid-beta pathology in C. elegans has been generated which allows for substoichiometric fluorescent labeling of amyloid-beta species in living nematodes. By microscopy, FLIM, physiological and biochemical studies, the progress of neurodegeneration and general pathogenicity of amyloidogenic peptides is confirmed. Notably, a single set of neurons was shown to be most vulnerable to Amyloid-beta overexpression and putatively act as a seed for systemic pathogeny. A muscular version of the amyloid-beta reporter strain was used to perform proteomics analysis from worm lysates after enrichment by immunoprecipitation, using anti-amyloid-beta antibodies.

2020-09-18 | PXD017590 | Pride

Plant seeds

Project description:Eight Plant seeds 16S raw sequence reads

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transcriptome and translatome of arabidopsis wt seeds according to dormancy-transcriptomic and translatomic analysis of Arabidopsis seeds

Project description:au13-12_polysome - transcriptome and translatome of arabidopsis wt seeds according to dormancy - Identification of transcripts that are differentially abundant (transcriptome) and transcripts that are addressed to translation (translatome) in imbibed Arabidopsis seeds in relation with dormancy. During imbibition of seeds (16h and 24h in darkness at 25°C, dormant and non-dormant seeds), transcriptome analysis is done on total RNA and translatome analysis on polysomal RNA. - At harvest seeds are dormant. They stay dormant if they are stored at -20°C (D) and become non-dormant (ND) if they are stored 3 weeks at +20°C. Arabidopsis dormant seeds do not germinate at 25°C in darkness while non-dormant seeds do. Total RNA and polysomal RNA (polysomal fractions purified on sucrose gradients) were extracted from imbibed seeds for 16h or 24h at 25°C in darkness (3 biological replicates). Transcriptome and translatome are compared for Dormant vs Non-Dormant for 16h and 24 imbibition. In silico comparison will allow to compare transcriptome and translatome for each point and type of seeds and to compare the time points (16 vs 24h) for each type of sample.

2014-09-27 | GSE61809 | GEO

Modulation of gene expression by complement protein C1q in amyloid-beta injured neurons

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2012-12-21 | E-GEOD-28886 | biostudies-arrayexpress

Expression data from dormant and after-ripened wheat seeds and after-ripened seeds imbibed in ABA.

Project description:Wheat seed dormancy is released by after-ripening, and germination and seminal root growth of after-ripened/non-dormant seeds can be inhibited by treatment with exogenous ABA. We used Affymetrix GeneChip Wheat Genome Array to detail transcriptional programs affected by after-ripening of dormant seeds and imbibition of after-ripened seeds in ABA.

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