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RNA sequencing of adipose tissue-derived stromal cells (ADSCs) with or without Sal-B addition

ABSTRACT: RNA sequencing of adipose tissue-derived stromal cells (ADSCs) with or without Sal-B addition

PROVIDER: PRJNA733677 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14687278_1.fastq.gz	Fastqsanger.gz
	SRR14687278_2.fastq.gz	Fastqsanger.gz
	SRR14687279_1.fastq.gz	Fastqsanger.gz
	SRR14687279_2.fastq.gz	Fastqsanger.gz
	SRR14687280_1.fastq.gz	Fastqsanger.gz

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RNA sequencing of adipose tissue-derived stromal cells (ADSCs) with or without Sal-B addition

Project description:Salvianolic acid B (Sal-B) is the most abundant and bioactive water-soluble compound in Salvia miltiorrhiza. Studies have shown that salvianolic acid B increases the mRNA expression of adipogenic transcription factors, including PPARγ, C/EBPα and PPARα, in 3T3-L1 preadipocytes to increase glucose uptake and mitochondrial respiration, reduce glycerol release and promote adipocyte differentiation. However, the effect of Sal-B on graft fat survival and its specific mechanism have not been investigated. So we dicided using h-ADSCs to see its transcriptomes changing after Sal-B addition.

2021-07-01 | GSE175795 | GEO

RNA sequencing of RAW264.7 with or without Sal-B and LPS addition

Project description:RNA sequencing of RAW264.7 with or without Sal-B and LPS addition

| PRJNA776638 | ENA

RNA sequencing of RAW264.7 with or without Sal-B and LPS addition

Project description:Autologous fat grafting (AFG), although an appealing approach to repair soft tissue defects, has various complications. Excessive inflammation at the transplant site is one of the main reasons for the poor effect of fat transplantation and occurrence of complications. Our previous study proved that Salvia miltiorrhiza can enhance fat graft survival. Salvianolic acid B (Sal-B) is the most abundant and bioactive water-soluble compound in Salvia miltiorrhiza and has anti-inflammatory effects on other diseases. Therefore, we hypothesized that salvianolic acid B could improve the effect of fat grafts by inhibiting inflammation.

2023-09-14 | GSE186886 | GEO

Expression data from human adipose-derived stem cells (ADSCs)

Project description:Adipose-derived stem cells are angiogenic and attractive for therapeutic angiogenesis. However, ADSCs contain several subsets according to expression pattern of surface markers. We identified CD271 (LNGFR) can isolate ADSCs with superior capacity for angiogenesis. Therefore, we aimed to compare gene expression profile between CD271+ and CD271- ADSCs.

2019-01-14 | GSE125012 | GEO

Comparison of gene expression profiles between human DFAT cells and ADSCs derived from same donors

Project description:Dedifferentiated fat (DFAT) cells, established in vitro from mature adipocytes, exhibit properties of multipotent mesenchymal stem/stromal cells (MSCs), such as the ability to differentiate into multiple mesenchymal lineages. Although DFAT cells exhibit certain properties of proliferative progeny, at present there is only limited knowledge about their characteristics as MSCs because those cells are considered to be potential artifacts of cell culture. To elucidate the identity of DFAT cells, we compared gene expression profiles of human DFAT cells and adipose-derived stem/stromal cells (ADSCs) established using adipose tissue from the same donors. Microarray analysis showed that the global RNA expression profiles of human DFAT cells were very similar to those of ADSCs, a representative MSC, despite being committed adipocyte progenitors. Subcutaneous adipose tissues that were obtained during surgical operation for non-malignant disease were donated by 3 patients after obtaining informed consent. Three sets of DFAT cells and ADSCs, each derived from adipose tissue from the same donor were used for RNA extraction and subsequent microarray analysis.

2013-06-12 | E-GEOD-47869 | biostudies-arrayexpress

Gene expression profiles of hexadecyl-4-124I-iodobenzoate(124I-HIB)-labeled adipose-derived stem cells (ADSCs) in vitro and in vivo

Project description:[1] Microarray analysis in the rat myocardial tissue: 124I-HIB transplanted MI model Vs. phosphate buffered saline (PBS) injected myocardial infarction (MI) model Vs. Sham operated model [2] Microarray analysis in the rat adipose derived stem cells: 124I-HIB-labeled ADSCs Vs. Unlabeled ADSCs [1] We investigated the change of gene expression profile in sham operated-, PBS injected- and 124I-HIB-labeled ADSCs transplanted myocardium in rat myocaridial infarction (MI) model. [2] We compared gene expression profile with 124I-HIB labeled ADSCs and unlabeled ADSCs in vitro.

2015-01-06 | E-GEOD-64692 | biostudies-arrayexpress

RNA sequence for macrodactyly ADSCs

Project description:Macrodactyly is a congenital malformation characterized by enlargement of bone and soft tissues in limbs, typically with excessive accumulation of adipose tissues. Although gain-of-function mutation of PIK3CA has been identified in macrodactyly, the mechanism of PIK3CA mutation in adipose accumulation is poorly understood. In this study, we found that adipocytes from macrodactyly were more hypertrophic than those observed in polydactyly. PIK3CA (H1047R) activating mutation and enhanced activity of PI3K/AKT pathway were detected in macrodactylous adipose-derived stem cells (Mac-ADSCs). To identify the key downstream effectors of PIK3CA activation-mediated adipogenesis in Mac-ADSCs, we examined the transcriptome of Mac-ADSCs, BYL-719 treated Mac-ADSCs and Pol-ADSCs by RNA-Seq analysis.

2020-06-05 | GSE151840 | GEO

Lactobacillus sp. SAL.1 isolate:SAL.1

Project description:Lactobacillus sp. SAL.1 isolate:SAL.1 Genome sequencing

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miRNAs in adipose tissue derived exosomes (Exo-AT) and adipose stem cells derived exosomes (Exo-ADSCs)

Project description:The emerging evidences support that exosome cargo miRNAs function as important regulators in cell differentiation. Therefore, in order to figure out the mechanism that Exo-AT mediated adipogenesis, we profiled miRNAs in Exo-AT using high-throughput sequencing (miRNA-seq). After trimming low-quality reads, contaminants, adaptors, and reads smaller than 15 nt, the remaining reads were mapped to merged pre-miRNA data bases. To identify the conserved miRNAs in Exo exosomes, miRNAs were aligned to miRBase v21. 148 and 154 types of known miRNAs in Exo-ADSCs and Exo-AT, respectively, were identified in the two replicates. Among these miRNAs, 103 miRNAs were simultaneously detected in both Exo-ADSCs and Exo-AT. Compared to Exo-ADSCs, 45 conserved miRNAs were enriched (expressed ≥ 2 folds, FDR<0.05) in Exo-AT. KEGG Pathway analysis was performed for the targets of the most 20 enriched miRNAs in Exo-AT (compared with Exo-ADSCs) to determine their potential function. Data showed that pathways that regulate adipogenesis such as Wnt signaling pathway, Insulin signaling pathway, MAPK signaling pathway, TGF-ß signaling pathway were enriched significantly for targets of Exo-AT miRNAs. Furthermore, 14 of 45 enriched miRNAs in Exo-AT (31.11%, such as miR-30a-5p, miR-148a-3p) were reported to participate in regulation of adipogenesis while 8 miRNAs (17.78%, such as miR-93-5p, miR-150-3p) that negatively control osteoblastic differentiation of MSC have been described.

2018-12-01 | GSE92313 | GEO

Effect of in vivo culture condition on the proliferation and differentiation of rat adipose-derived stromal cells

Project description:Adipose-derived stromal cells (ADSCs) represent a promising stem cell source for tissue engineering and cell based therapy. However, long-term in vitro expansion of ADSCs impede stemness maintaining, partly attributed to deprivation of their original microenvironment. Incompetent cells largely limit the therapeutic effects in ADSCs based clinical use. Therefore, reconstructing a more physiologically and physically relevant niche is an ideal strategy to settle this issue and therefore facilitates their extensive applications in clinical. Here, we transplanted separated ADSCs to local subcutaneous adipose tissues of nude mice as an in vivo cell culture model. We found transplanted ADSCs maintained the primitive morphologies, showed improved proliferation and delayed senescence when compared to cells in conventional incubator. Significantly increased expression of stemness-related markers and multi-lineage differentiation abilities were further observed in in vivo cultured ADSCs. At last, sequencing results revealed different expression genes between ADSCs obtained from in vivo and in vitro conditions mainly located in extracellular matrix and extracellular space, which participated in regulating transcription and protein synthesis. Moreover, we found an Egr1 involved signaling pathway might exert a crucial impact on controlling stemness properties. Our findings might collectively pave the way between the bench and beside in regard of ADSCs based application.

2024-03-20 | GSE261680 | GEO

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