Project description:Kruppel-like factor 4 (KLF4), as a eukaryotic transcription factor, participates in the regulation of cell proliferation, differentiation, embryonic development and other important life processes. KLF4 is also closely related to the occurrence and development in variety types of tumor. To determine the functions of KLF4 in breast cancer, we over-expressed KLF4 in breast cancer LM2-4175 cells and used gene expression profiling to identify KLF4 regulated genes.
Project description:Klf4 and Sox2 are among the core reprogramming factor, and have different function in reprogramming. Klf4 is supposed to trigger MET in the early stage of reprogramming ,Sox2 is well known plutipotent relative gene. Klf4 and Sox2 play important role in induced reprogramming. We performed this experiment to identify the targets regulated by Sox2 and Klf4 respectively.