Alpine pastures environmental samples, including soil, feces and invertebrates
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PROVIDER: PRJNA734187 | ENA |
REPOSITORIES: ENA
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Project description:Contrasting levels of beta-diversity and underlying phylogenetic trends indicate different paths to chemical diversity in highland and lowland willow species
Martin Volf*, Jing Vir Leong, Paola de Lima Ferreira, Tereza Volfova, Petr Kozel, Pavel Matos-Maravi, Elvira Horandl, Natascha D. Wagner, Niko Luntamo, Juha-Pekka Salminen, Simon T. Segar, Brian E. Sedio
*Corresponding author: Martin Volf, volf@entu.cas.cz, tel. +420 387775038, Biology Centre, Czech Academy of Sciences, Institute of Entomology, Branisovska 31, 37005 Ceske Budejovice, Czech Republic
Untargeted metabolomics data was generated for the above publication for 29 species of willows in the Czech Republic and Austria, encompassing an elevation gradient spanning >2500 m (from 175 to 2620 m asl). Extraction and instrumental methods are reported in Volf et al. 2023 Ecology Letters and Sedio et al. 2021 Frontiers in Ecology and Evolution, https://doi.org/10.3389/fevo.2021.679638
2023-04-21 | MSV000091773 | MassIVE
Project description:Bifidobacteria have been recommended as potential indicators of human fecal pollution in surface waters even though very little is known about their presence in nonhuman fecal sources. The objective of this research was to shed light on the occurrence and molecular diversity of this fecal indicator group in different animals and environmental waters. Genus- and species-specific 16S rRNA gene PCR assays were used to study the presence of bifidobacteria among 269 fecal DNA extracts from 32 different animals. Twelve samples from three wastewater treatment plants and 34 water samples from two fecally impacted watersheds were also tested. The species-specific assays showed that Bifidobacterium adolescentis, B. bifidum, B. dentium, and B. catenulatum had the broadest host distribution (11.9 to 17.4%), whereas B. breve, B. infantis, and B. longum were detected in fewer than 3% of all fecal samples. Phylogenetic analysis of 356 bifidobacterial clones obtained from different animal feces showed that ca. 67% of all of the sequences clustered with cultured bifidobacteria, while the rest formed a supercluster with low sequence identity (i.e., <94%) to previously described Bifidobacterium spp. The B. pseudolongum subcluster (>97% similarity) contained 53 fecal sequences from seven different animal hosts, suggesting the cosmopolitan distribution of members of this clade. In contrast, two clades containing B. thermophilum and B. boum clustered exclusively with 37 and 18 pig fecal clones, respectively, suggesting host specificity. Using species-specific assays, bifidobacteria were detected in only two of the surface water DNA extracts, although other fecal anaerobic bacteria were detected in these waters. Overall, the results suggest that the use of bifidobacterial species as potential markers to monitor human fecal pollution in natural waters may be questionable.
| S-EPMC2227705 | biostudies-literature
Project description:Metagenomic analysis of environmental feces
| PRJNA772888 | ENA
Project description:BackgroundMultiple infection sources for enterohemorrhagic Escherichia coli O157:H7 (EHEC) are known, including animal products, fruit and vegetables. The ecology of this pathogen outside its human host is largely unknown and one third of its annotated genes are still hypothetical. To identify genetic determinants expressed under a variety of environmental factors, we applied strand-specific RNA-sequencing, comparing the SOLiD and Illumina systems.ResultsTranscriptomes of EHEC were sequenced under 11 different biotic and abiotic conditions: LB medium at pH4, pH7, pH9, or at 15°C; LB with nitrite or trimethoprim-sulfamethoxazole; LB-agar surface, M9 minimal medium, spinach leaf juice, surface of living radish sprouts, and cattle feces. Of 5379 annotated genes in strain EDL933 (genome and plasmid), a surprising minority of only 144 had null sequencing reads under all conditions. We therefore developed a statistical method to distinguish weakly transcribed genes from background transcription. We find that 96% of all genes and 91.5% of the hypothetical genes exhibit a significant transcriptional signal under at least one condition. Comparing SOLiD and Illumina systems, we find a high correlation between both approaches for fold-changes of the induced or repressed genes. The pathogenicity island LEE showed highest transcriptional activity in LB medium, minimal medium, and after treatment with antibiotics. Unique sets of genes, including many hypothetical genes, are highly up-regulated on radish sprouts, cattle feces, or in the presence of antibiotics. Furthermore, we observed induction of the shiga-toxin carrying phages by antibiotics and confirmed active biofilm related genes on radish sprouts, in cattle feces, and on agar plates.ConclusionsSince only a minority of genes (2.7%) were not active under any condition tested (null reads), we suggest that the assumption of significant genome over-annotations is wrong. Environmental transcriptomics uncovered hitherto unknown gene functions and unique regulatory patterns in EHEC. For instance, the environmental function of azoR had been elusive, but this gene is highly active on radish sprouts. Thus, NGS-transcriptomics is an appropriate technique to propose new roles of hypothetical genes and to guide future research.
| S-EPMC4048457 | biostudies-literature
Project description:Ocean warming (OW) and acidification (OA) are key features of global change and are predicted to have negative consequences for marine species and ecosystems. At a smaller scale increasing oil and gas activities at northern high latitudes could lead to greater risk of petroleum pollution, potentially exacerbating the effects of such global stressors. However, knowledge of combined effects is limited. This study employed a scenario-based, collapsed design to investigate the impact of one local acute stressor (North Sea crude oil) and two chronic global drivers (pH for OA and temperature for OW), alone or in combination on aspects of the biology of larval stages of two key invertebrates: the northern shrimp (Pandalus borealis) and the green sea urchin (Strongylocentrotus droebachiensis). Both local and global drivers had negative effects on survival, development and growth of the larval stages. These effects were species- and stage-dependent. No statistical interactions were observed between local and global drivers and the combined effects of the two drivers were approximately equal to the sum of their separate effects. This study highlights the importance of adjusting regulation associated with oil spill prevention to maximize the resilience of marine organisms to predicted future global conditions.
| S-EPMC6255813 | biostudies-literature
Project description:Purpose: The goals of this study are to compare the differentially expressed miRNAs between SW480 and SW620. Methods:Total RNA of two samples was used to prepare the miRNA sequencing library.The libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 50 cycles on Illumina NextSeq. Results:We found 72 miRNAs were downregulated on the basis of fold-change less than 0.5. However,total 160 miRNAs were upregulated on the basis of fold-change greater than 2. Conclusions: Our study represents the detailed analysis of SW480 and SW620, generated by RNA-seq technology. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of miRNA content. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.
2020-01-22 | GSE144002 | GEO
Project description:Contrasting levels of beta-diversity and underlying phylogenetic trends indicate different paths to chemical diversity in highland and lowland willow species
Martin Volf*, Jing Vir Leong, Paola de Lima Ferreira, Tereza Volfova, Petr Kozel, Pavel Matos-Maravi, Elvira Horandl, Natascha D. Wagner, Niko Luntamo, Juha-Pekka Salminen, Simon T. Segar, Brian E. Sedio
*Corresponding author: Martin Volf, volf@entu.cas.cz, tel. +420 387775038, Biology Centre, Czech Academy of Sciences, Institute of Entomology, Branisovska 31, 37005 Ceske Budejovice, Czech Republic
Untargeted metabolomics data was generated for the above publication for 29 species of willows in the Czech Republic and Austria, encompassing an elevation gradient spanning >2500 m (from 175 to 2620 m asl). Extraction and instrumental methods are reported in Volf et al. 2023 Ecology Letters and Sedio et al. 2021 Frontiers in Ecology and Evolution, https://doi.org/10.3389/fevo.2021.679638
2023-04-21 | MSV000091773 | GNPS