Catabolism of strigolactones by a carboxylesterase
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA734575 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR14711167_1.fastq.gz | Fastqsanger.gz | |||
| SRR14711167_2.fastq.gz | Fastqsanger.gz | |||
| SRR14711168_1.fastq.gz | Fastqsanger.gz | |||
| SRR14711168_2.fastq.gz | Fastqsanger.gz | |||
| SRR14711169_1.fastq.gz | Fastqsanger.gz |
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Project description:Strigolactones (SLs) are carotenoid-derived plant hormones that control shoot branching and communications between host plants and symbiotic fungi or root parasitic plants. Extensive studies have identified the key components participating in SL biosynthesis and signaling, whereas the catabolism or deactivation of endogenous SLs in planta remains largely unknown. Here we report that the Arabidopsis carboxylesterase 15 (AtCXE15) and its orthologues function as efficient hydrolases of SLs. We show that overexpression of AtCXE15 promotes shoot branching by dampening SL-inhibited axillary bud outgrowth. We further demonstrate that AtCXE15 could bind and efficiently hydrolyze SLs both in vitro and in planta. We also provide evidence that AtCXE15 is capable to catalyze hydrolysis of diverse SL analogues and that such CXE15-dependent catabolism of SLs is evolutionarily conserved in seed plants. These results disclose a catalytic mechanism underlying homeostatic regulation of SLs in plants, which also provides a rational approach to spatial-temporally manipulate the endogenous SLs and thus architecture of crops and ornamental plants.
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Project description:In response to environmental stressors, plants exhibit a phenomenon known as stress memory, whereby a prior stress episode influences their subsequent responses. This suggests the existence of a molecular memory mechanism that allows plants to adapt to recurring stress events. Previous work has highlighted the contribution of the phytohormone strigolactones to drought stress and memory thereof (10.1007/s00425-015-2266-8; 10.1111/nph.14190; 10.1111/pce.13758; 10.1093/pcp/pcac058). Additionally, there are sparse indications that especially (but not only) in Solanaceous plants, they may have a significant effect on reproduction (reviewed by 10.1111/pce.14461). However, the molecular underpinnings of both phenomena have not been researched yet. This study investigated the positioning of strigolactones to the tomato flowering network, and their contribution to drought memory.
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Project description:Autophagy, involved in protein degradation and amino acid recycling, plays a key role in plant development and stress responses. However, the relationship between autophagy and phytohormones is unclear. We used diverse methods, including CRISPR/Cas9, UPLC-MS/MS, chromatin immunoprecipitation, electrophoretic mobility shift assays, and dual-luciferase assays to explore the molecular mechanism of strigolactones in regulating autophagy and the degradation of ubiquitinated proteins under cold stress in tomato. We show that cold stress induced the accumulation of ubiquitinated proteins. Mutants deficient in strigolactone biosynthesis were more sensitive to cold stress with a higher accumulation of ubiquitinated proteins, while treatment with the synthetic strigolactone analog GR245DS enhanced cold tolerance in tomato, with a higher accumulation of autophagosomes and transcripts of autophagy-related genes (ATGs), and a lower accumulation of ubiquitinated proteins. Meanwhile, cold stress induced ELONGATED HYPOCOTYL 5 (HY5) accumulation, which was triggered by strigolactones. HY5 further trans-activated ATG18a promoter, resulting in autophagy formation. Mutation of ATG18a compromised strigolactone-induced cold tolerance, followed by a decreased formation of autophagosomes and an increased accumulation of ubiquitinated proteins. These findings reveal that strigolactones positively regulate autophagy in an HY5-dependent manner and promote the degradation of ubiquitinated proteins under cold conditions in tomato.
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