Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bulk RNAsequencing of H9 Primed and Naïve Pluripotent Stem Cells with and without EZH2 inhibition.

ABSTRACT: Bulk RNAsequencing of H9 Primed and Naïve Pluripotent Stem Cells with and without EZH2 inhibition.

PROVIDER: PRJNA735229 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14735600.fastq.gz	Fastqsanger.gz
	SRR14735601.fastq.gz	Fastqsanger.gz
	SRR14735602.fastq.gz	Fastqsanger.gz
	SRR14735603.fastq.gz	Fastqsanger.gz
	SRR14735604.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Bulk RNAsequencing of H9 Primed and Naïve Pluripotent Stem Cells with and without EZH2 inhibition.

Project description:Bulk RNAseq of 3 replicates of primed H9 +/- EZH2i (E8Flex) and 3 replicates of naive H9 +/- EZH2i (PXGL) to assess the effect of EZH2i in human pluripotent stem cells

2022-03-23 | GSE176172 | GEO

Single-Cell RNAsequencing of Human Naïve Pluripotent Stem Cells to Trophoblast Stem Cells with and without EZH2 inhibition.

Project description:Single-Cell RNAsequencing of the conversion of naïve iCas9 Sigma hiPSC line 1 (iCSIG-1 hiPSCs) into trophoblast stem cells with and without EZH2i (Abcam ab146152 UNC1999, EZH2 methyltransferase inhibitor) during 4 days preconversion and during 4 days during conversion (total of 8 days with or without PRC2i).

2022-03-23 | GSE176173 | GEO

CUT & RUN H3K27me3 of Human Naïve and Primed Pluripotent Stem Cells with and without EZH2 inhibition.

Project description:CUT & RUN H3K27me3 of Human Naïve and Primed Pluripotent Stem Cells with and without EZH2 inhibition.

2022-03-23 | GSE183735 | GEO

Translational and Post-translational LC-MS of Naïve and Primed hESCs

Project description:Deciphering the regulatory network for human naïve and primed pluripotency is of fundamental theoretical and applicable significance. Here, by combining quantitative proteomics, phosphoproteomics and acetylproteomics analyses, we revealed RNA processing and translation as the most differentially-regulated processes between naïve and primed human embryonic stem cells (hESCs). While glycolytic primed hESCs rely predominantly on eIF4E-mediated cap-dependent pathway for protein translation, naïve hESCs with reduced mTORC1 activity are more tolerant to blockage of eIF4E-dependent translation, and their bivalent metabolism allows for translating selective mRNAs via both eIF4E-dependent and eIF4E-independent/eIF4A2-dependent pathways to form a more compact naïve proteome. Globally up-regulated proteostasis system and down-regulated post-translational modification system help to further refine and maintain the naïve proteome that is compatible with the more rapid cycling of naïve hESCs, where CDK1 plays an indispensable coordinative role.

2021-12-10 | PXD030230 | Pride

The translational landscape of naïve and primed state pluripotency

Project description:Translational control plays a central role in regulation of gene expression and can lead to significant divergence between mRNA- and protein-abundance. The translational landscape of early mammalian development and its impact on cellular proteome, however, remains largely un-explored. Here we used genome-wide approaches combined with time-course analysis to measure the mRNA-abundance, mRNA-translation rate and protein expression during the transition of naïve into primed embryonic stem cells (ESCs). We found that the ground state ESCs cultured with GSK3- and MEK-inhibitors and LIF (2iL) display higher ribosome density on a selective set of mRNAs. These mRNAs show reduced translation during the exit from ground state pluripotency and transition to serum/LIF (SL) culture or upon commitment to primed epiblast-like stem cells (EpiLSCs). Strikingly, integrative analysis with cellular proteome indicate a strong translational buffering of this set of mRNAs in 2iL-ESCs leading to stable protein expression levels. Our data reveal that the global alteration of cellular proteome is largely accompanied by transcriptional rewiring. Furthermore, we identified a set of genes (including UHRF1 and KRAS) that undergo selective post-translational regulation during the transition of naïve into primed pluripotency and linked the observed changes to upstream GSK- and MEK/MAPK-signaling pathways using single inhibitor treated ESCs. Thus, we provide a comprehensive and detailed overview of the global changes in gene expression during the transition of naïve to primed pluripotency and dissect the relative contributions of RNA-transcription, translation and regulation of protein stability in controlling protein abundance.

2020-04-03 | PXD014528 | Pride

Naïve and Primed MSC derived exosomes

Project description:Naïve and Primed MSC derived exosomes

| PRJNA575856 | ENA

Naïve and Primed MSC derived exosomes

Project description:Here we report the change in gene expression in naïve and primed MSC exosomes

2021-06-29 | GSE138435 | GEO

Single-Cell RNAsequencing of Human Naïve Pluripotent Stem Cells to Trophoblast Stem Cells with and without EZH2 inhibition.

Project description:Single-Cell RNAsequencing of Human Naïve Pluripotent Stem Cells to Trophoblast Stem Cells with and without EZH2 inhibition.

| PRJNA735230 | ENA

OGA-mediated protein glycosylation regulates pluripotency states in human embryonic stem cells

Project description:In our previous study, we uncovered that OGA significantly elevated in naïve hESC. Therefore, we sought to investigate the effects of O-GlcNAc on hESC and the interconvert of both pluripotent states. Depletion of OGA, which results in global O-GlcNAcylation increased, would impair naïve hESC pluripotency but can promote naïve to primed hESC transition. In addition, we obtained the profiles of O-GlcNAcylated proteins in H9 primed hESC and H9-PXGL hESC via MS based-quantitative proteomics.

2024-08-10 | PXD041605 | Pride

CUT & RUN H3K27me3 of Human Naïve and Primed Pluripotent Stem Cells with and without EZH2 inhibition.

Project description:CUT & RUN H3K27me3 of Human Naïve and Primed Pluripotent Stem Cells with and without EZH2 inhibition.

| PRJNA761779 | ENA