Ontology highlight
ABSTRACT:
PROVIDER: PRJNA735446 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR14785186_1.fastq.gz | Fastqsanger.gz | |||
| SRR14785186_2.fastq.gz | Fastqsanger.gz | |||
| SRR14785187_1.fastq.gz | Fastqsanger.gz | |||
| SRR14785187_2.fastq.gz | Fastqsanger.gz | |||
| SRR14785188.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Carbon (C), nitrogen (N) and phosphorus (Pi) are crucial macronutrients for plants. While substantial knowledge exists on C/N balance signaling physiological and molecular perspectives, the core regulatory mechanism governing C/N and Pi balance remains largely unexplored. This study reveals that C/N assimilatory metabolisms matching the Pi-deficiency stress is integrated by a DEEP GREEN PANICLE1 (DGP1)-mediated regulatory network in rice (Oryza sativa). We found that low-Pi stress triggers DGP1 expression in a dose-responsive manner, facilitated by the PHOSPHATE STARVATION RESPONSE REGULATOR (PHR2). Intriguingly, DGP1 interacts with transcription factors (GLK1 and GLK2) and mitochondrial factors (GDCH and QCR9), modulating their roles in photosynthetic gene expression, the glycine cleavage system and the mitochondrial electron transport chain (mETC), respectively. Additionally, DGP1 influences aminoacyl-tRNA synthetases and ribosomal subunits, impacting protein biosynthesis, and intervenes in various metabolic pathways, including glycolysis and the citrate cycle. Phenotypically, we observed that under short-term Pi-deficiency, wild-type plants exhibit compromised photosynthesis, photorespiration and mETC-driven ATP synthesis, whereas dgp1 and phr2 mutants display reduced sensitivity to these conditions. Conversely, DGP1 overexpressors show decreased yield under normal conditions due to altered cell metabolomes and protein biosynthesis inadequacy, despite unaffected 15NO3– uptake and transport. In conclusion, the PHR2-DGP1 module orchestrates a comprehensive signaling network, harmonizing C and N metabolic fluxes under Pi limitation through coordinated interactions among cytoplast, chloroplast and mitochondria.
2025-01-27 | GSE253911 | GEO
Project description:Carbon (C), nitrogen (N) and phosphorus (Pi) are crucial macronutrients for plants. While substantial knowledge exists on C/N balance signaling physiological and molecular perspectives, the core regulatory mechanism governing C/N and Pi balance remains largely unexplored. This study reveals that C/N assimilatory metabolisms matching the Pi-deficiency stress is integrated by a DEEP GREEN PANICLE1 (DGP1)-mediated regulatory network in rice (Oryza sativa). We found that low-Pi stress triggers DGP1 expression in a dose-responsive manner, facilitated by the PHOSPHATE STARVATION RESPONSE REGULATOR (PHR2). Intriguingly, DGP1 interacts with transcription factors (GLK1 and GLK2) and mitochondrial factors (GDCH and QCR9), modulating their roles in photosynthetic gene expression, the glycine cleavage system and the mitochondrial electron transport chain (mETC), respectively. Additionally, DGP1 influences aminoacyl-tRNA synthetases and ribosomal subunits, impacting protein biosynthesis, and intervenes in various metabolic pathways, including glycolysis and the citrate cycle. Phenotypically, we observed that under short-term Pi-deficiency, wild-type plants exhibit compromised photosynthesis, photorespiration and mETC-driven ATP synthesis, whereas dgp1 and phr2 mutants display reduced sensitivity to these conditions. Conversely, DGP1 overexpressors show decreased yield under normal conditions due to altered cell metabolomes and protein biosynthesis inadequacy, despite unaffected 15NO3– uptake and transport. In conclusion, the PHR2-DGP1 module orchestrates a comprehensive signaling network, harmonizing C and N metabolic fluxes under Pi limitation through coordinated interactions among cytoplast, chloroplast and mitochondria.
2025-01-27 | GSE253909 | GEO
Project description:Inorganic phosphate (Pi) is an essential nutrient, which is often served as a limiting factor in plant growth. It has been reported that SPL family members, such as SPL3, regulate Pi deficiency responses by controlling the expression of Pi deficiency responsive genes. To elucidate whether SPL9 respond to low phosphorus stress, we investigated the phenotypes and conduct RNA sequencing analysis in transgenic Arabidopsis thaliana with overexpressing SPL9 (R9) under conditions of both normal and low Pi availability. Compared with wild-type plants, R9 showed decreased anthocyanin accumulation and increased Pi contents in shoots under Pi deficiency. Through RNA-seq analysis compared with wild-type plants, we detected 217 genes significantly differentially expressed in conditions of Pi sufficiency, and 121 genes differentially expressed in conditions of Pi deficiency in R9 plants. Under Pi deficiency, these genes included multiple protein kinases, jasmonic acid response genes and genes related to salt stress responses. Genes associated with hydrolase and transferase activity were also differentially regulated by Pi deficiency, such as cytochrome P450 monooxygenases. Of particular note, the transcription factor AP2-EREBP and members of the bHLH family were among the most significantly differentially regulated genes identified under both Pi sufficient and Pi deficient conditions.
2021-09-03 | GSE183312 | GEO
Project description:In this study, proteomics was used to sequence the salt stress treatment group and the control group of Medicago sativa and Medicago truncatula. The aim was to discover the kegg pathway of the two alfalfa varieties under salt stress, which was of great significance to the exploration of the salt tolerance mechanism of alfalfa.
2023-12-09 | PXD047690 |
Project description:When grown under phosphate (Pi) deficiency, plants adjust their developmental program and metabolic activity to cope with this nutritional stress. For Arabidopsis, the developmental responses include inhibition of primary root growth and enhanced formation of lateral roots and root hairs. Pi deficiency also inhibits photosynthesis by suppressing the expression of photosynthetic genes. Interestingly, early studies showed that photosynthetic gene expression was also suppressed in roots, a non-photosynthetic tissue. The biological relevance of this phenomenon, however, is not known. In this work, we characterized an Arabidopsis mutant, hps7, which is hypersensitive to Pi deficiency; the hypersensitivity includes an increased inhibition of root growth. HPS7 encodes a tyrosylprotein sulfotransferase (TPST). Accumulation of TPST proteins, but not mRNA, is induced by Pi deficiency. Comparative RNA-Seq analyses indicated that expression of many photosynthetic genes was activated in the roots of hps7. Under Pi deficiency, the expression of the photosynthetic genes in hps7 is further increased, which leads to the enhanced accumulation of chlorophyll, starch, and reactive oxygen species. The increased inhibition of root growth in hps7 under Pi deficiency was completely reversed by growing plants in the dark. Based on these results, we propose that suppression of photosynthetic gene expression in roots is required for sustained root growth under Pi deficiency.
2014-07-28 | E-MTAB-4405 | biostudies-arrayexpress
Project description:After 24 h of acclimatisation Medicago sativa seedlings were treated with HgCl2 diluted on fresh MS to a final concentration of 3 uM during 3, 6 and 24 h.
2014-03-03 | E-MEXP-3876 | biostudies-arrayexpress
Project description:The objective of this project is to identify genes that are expressed in the Arabidopsis thaliana root tip and that are early induced (or repressed) by phoshate deficiency. Seedlings were germinated and grown (for 6 days) on phosphate rich medium and transfered to either a phosphate poor medium (Pi- = 20uM Pi treatment) or a phosphate rich medium (Pi+ = 500uM, Pi = control). Fifteen and 60 minutes after transfer, the tip (~800um) of the primary root was cut under a dissecting microscope. About 100 root tips per condition were harvested and there mRNA was analysed with the use of microarrays.
2011-09-09 | GSE31135 | GEO
Project description:After 24 hours of germination, Medicago sativa seedlings were grown with MS nutrient solution and 0, 50, 100 and and 200 microM Na2HAsO4 in a hydroponic system, for 24 hours. Roots were harvest for mRNA extraction and transcritpional analysis.
2015-07-31 | E-MTAB-2158 | biostudies-arrayexpress
Project description:To better understand the complex mechanisms regulating Pi homeostasis in rice (Oryza sativa L. cv. Nipponbare), a time course experiment was performed, where pre-germinated seedlings were grown hydroponically for two weeks on Pi-sufficient medium (0.32 mM Pi), before transferring half of the plants to Pi-deficient solution (0 mM Pi) for 21 days (d). After three weeks of Pi-starvation treatment, half of these plants where then re-supplied with Pi sufficient media for up to 24 hours (h). In total, nine time points were selected in order to cover short and long term responses to Pi starvation as well as the effects of Pi re-supply on Pi starved plants.
2013-11-25 | E-MTAB-4427 | biostudies-arrayexpress