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Single-Cell Transcriptome Analysis Reveals Differential Signatures of Human Left-Sided and Right-Sided Colon

ABSTRACT: Single-Cell Transcriptome Analysis Reveals Differential Signatures of Human Left-Sided and Right-Sided Colon

PROVIDER: PRJNA736439 | ENA |

REPOSITORIES: ENA

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Single-Cell Transcriptome Analysis Reveals Differential Signatures of Human Left-Sided and Right-Sided Colon

Project description:In our study, we obtained paired normal left-sided and right-sided colon mucosa tissues by colonoscopy, and 9,642 individual cells were analyzed by single-cell RNA-seq, which aims to explore the differennces of cell component between the left and the right colon.

2024-06-10 | GSE176499 | GEO

serum exosomes of right-sided colon cancer promote metastasis via overexpression of SPARC and LRG1

Project description:Purpose: Over the past few years, the distinction between left- and right-sided colon cancer has been brought into focus. Right-sided tumor location was associated with an inferior overall survival and progression-free survival. We aimed to perform a detailed analysis of the diversity in exosomes between left- and right-sided colon cancer using quantitative proteomics. Experimental Design: We isolated exosomes from left- and right-sided colon cancer patients and healthy volunteers and treated colorectal cancer cell line with serum-derived exosomes. Then we performed quantitative proteomics analysis of the serum-derived exosomes and cell line treated with exosomes, respectively. Results: The expression profile of the serum exosome proteome in patients with right-sided colon cancer is different from patients with left-sided colon cancer. Serum-derived exosomes of right-sided colon cancer promote metastasis via up-regulation of extracellular matrix-related proteins, especially proteoglycans like SPARC and glycoprotein like LRG1. Exosomal SPARC and LRG1 were closely correlated with progression-free survival. Conclusions: Proteomic analysis identified different exosomal protein profiling between left- and right-sided colon cancer. Serum-derived exosomes of right-sided colon cancer promote metastasis via overexpression of SPARC and LRG1.

2019-11-25 | PXD012304 | Pride

Resolving the Difference Between Left-sided and Right-sided Colorectal Cancer by Single-cell Sequencing

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serum exosomes contain SPARC and LRG1 as biomarkers for colon cancer and differ by tumor primary location

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PLA2G4A promotes right-sided colorectal cancer progression by inducing CD39+γδ Tregs polarization

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Project description:Loss of SUV420H2-dependent chromatin compaction drives right-sided colon cancer progression

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Loss of SUV420H2-dependent chromatin compaction drives right-sided colon cancer progression [CGH]

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