Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide knockout screen of OC43/SARS-CoV2-infected HEK293-hACE2 and Vero E6 cells

ABSTRACT: Genome-wide knockout screen of OC43/SARS-CoV2-infected HEK293-hACE2 and Vero E6 cells

PROVIDER: PRJNA737123 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14794424_1.fastq.gz	Fastqsanger.gz
	SRR14794424_2.fastq.gz	Fastqsanger.gz
	SRR14794425_1.fastq.gz	Fastqsanger.gz
	SRR14794425_2.fastq.gz	Fastqsanger.gz
	SRR14794426_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 122

Similar Datasets

Inhibition of coronavirus HCoV-OC43 by targeting the eIF4F complex [Vero E6]

Project description:This study reports a screen to identify putative inhibitors of the eIF4F complex for potential effects on blocking coronavirus replication, using HCoV-OC43 (OC43) infection of Vero E6 cells and the lung epithelial cancer line A549 as models.

2022-12-13 | GSE198399 | GEO

Genome-wide knockout screen of OC43/SARS-CoV2-infected Vero E6 cells

Project description:Genome-wide knockout screen of OC43/SARS-CoV2-infected Vero E6 cells

| PRJNA737124 | ENA

Inhibition of coronavirus HCoV-OC43 by targeting the eIF4F complex [A549]

2022-12-13 | GSE198398 | GEO

Inhibition of coronavirus HCoV-OC43 by targeting the eIF4F complex [Vero E6]

Project description:Inhibition of coronavirus HCoV-OC43 by targeting the eIF4F complex [Vero E6]

| PRJNA814883 | ENA

Genome-wide knockout screen of OC43/SARS-CoV2-infected HEK293-hACE2 cells I

Project description:Genome-wide knockout screen of OC43/SARS-CoV2-infected HEK293-hACE2 cells I

| PRJNA737125 | ENA

Genome-wide knockout screen of OC43/SARS-CoV2-infected HEK293-hACE2 cells II

Project description:Genome-wide knockout screen of OC43/SARS-CoV2-infected HEK293-hACE2 cells II

| PRJNA737127 | ENA

Genome-wide knockout screen of OC43/SARS-CoV2-infected HEK293-hACE2 and Vero E6 cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-12-22 | GSE177545 | GEO

Transcriptome analysis to SARS-CoV-2 infection in Vero E6 cells

Project description:RNAseq was used to identify host and viral transcriptome changes in SARS-CoV-2 infection in Vero E6 cells at 8hpi.

2020-11-21 | GSE161881 | GEO

Gene expression of SARS-CoV-2-infected Vero E6 cells

Project description:We conducted a high-throughput drug repositioning screen using the LOPAC®1280 and the ReFRAME drug libraries to identify existing drugs that harbor antiviral activity against SARS-CoV-2, in a Vero E6 cell-based assay. We additionally performed RNA sequencing on control and SARS-CoV-2 infected Vero E6 cells to study the biological changes after SARS-CoV-2 infection and to elucidate the potential mechanisms underlying the positive hits identified from our high-throughput screen. Vero E6 cells were either mock-infected or infected with SARS-CoV-2 USA-WA1/2020 (MOI = 0.3) with three replicates. Cells were harvested 24 hours after infection, and total RNA was extracted using the Qiagen® RNeasy® Plus Mini Kit. The quality of the extracted RNA was assessed with the Agilent® 2100 Bioanalyzer. Libraries were prepared from total RNA following ribosome RNA depletion using standard protocol according to Illumina®. Total RNA sequencing was then performed on the Illumina® NextSeq system; 150bp paired-end runs were performed and 100 million raw reads per sample were generated.

2020-07-08 | GSE153940 | GEO

RNA-Seq Quantitative Analysis of the regulation of SARS-CoV-2 infection on autophagy and apoptosis related gene transcriptome in infected cells

Project description:In our study, we found that SARS-CoV-2-S pseudovirions infection induced high levels of autophagy and apoptosis in infected cells. To further investigate the underlying regualtion of SARS-CoV-2-S pseudovirions infection in infected cells, ACE2-expressing HEK293T-hACE2 and Vero E6 cells were treated with Mock or SARS-CoV-2-S pseudovirions for RNA-Seq analysis to exame the expression of autophagy- and apoptosis-related genes. Our results indicate that the majority of autophagy- and apoptosis-promoting genes were significantly increased in SARS-CoV-2-S pseudovirions treated HEK293T-hACE2 and Vero E6 cells. In contrast, the subset of autophagy- and apoptosis-suppressing genes was significantly decreased in SARS-CoV-2-S pseudovirions-treated HEK293T-hACE2 and Vero E6 cells than Mock-treated HEK293T-hACE2 and Vero E6 cells. Meanwhile, SARS-CoV-2-S pseudovirions infection enhanced the expression of pro-inflammatory cytokines in infected cells.

2021-03-23 | GSE169370 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data