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Comparison of genomic binding profile for CFIm25 in control and CFIm25 KD hESCs (H9 cell line)

ABSTRACT: Comparison of genomic binding profile for CFIm25 in control and CFIm25 KD hESCs (H9 cell line)

PROVIDER: PRJNA737294 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14802456_1.fastq.gz	Fastqsanger.gz
	SRR14802456_2.fastq.gz	Fastqsanger.gz
	SRR14802457_1.fastq.gz	Fastqsanger.gz
	SRR14802457_2.fastq.gz	Fastqsanger.gz
	SRR14802458_1.fastq.gz	Fastqsanger.gz

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poly(A+)-seq profiling of gene expression in control H9 and (CFIm25 KD+ 3Flag-CFIm25 overexpression) H9 cell lines

Project description:We carried out CFIm25 KD experiments in H9 cell line (hESCs). After poly(A+)-seq, we found the expression of hundreds of genes were changed. To investigate if the effect is a direct or indirect effect of CFIm25 KD, we next carried out 3Flag-CFIm25 overexpression in CFIm25 KD cells for gene-rescue experiments. After we obtained this cell line, here we performed poly(A+)-seq in control H9 and (CFIm25 KD+ 3Flag-CFIm25 overexpression)H9 cell lines. The goal of this experiment is to investigate if CFIm25 overexpression could rescue the gene expression phenotype caused by CFIm25 KD.

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Comparison of genomic binding profile for CFIm25 in control and CFIm25 KD hESCs (H9 cell line)

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