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Single-cell polyadenylation sequencing (scPolyA-seq) reveals the dynamic of polyA site usage during cell cycles in tumor cell lines [C1]

ABSTRACT: Single-cell polyadenylation sequencing (scPolyA-seq) reveals the dynamic of polyA site usage during cell cycles in tumor cell lines [C1]

PROVIDER: PRJNA738159 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14820635_1.fastq.gz	Fastqsanger.gz
	SRR14820635_2.fastq.gz	Fastqsanger.gz
	SRR14820636_1.fastq.gz	Fastqsanger.gz
	SRR14820636_2.fastq.gz	Fastqsanger.gz
	SRR27505800_1.fastq.gz	Fastqsanger.gz

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Single-cell polyadenylation sequencing (scPolyA-seq) reveals the dynamic of polyA site usage during cell cycles in tumor cell lines [C1]

Project description:Alternative polyadenylation (APA) plays an important role in post-transcriptional gene regulation. But how genes' APA usages are influenced by cell cycle remains largely unknown. Here we developed single cell polyadenylation sequencing (scPolyA-seq), a strand-specific approach for sequencing 3’ end of transcripts which can get very deep sequencing level around polyA site for each cell, to investigate the landscape of APA at single cell level, based on Fluidigm C1. We compared APA changes before and after thymidine double blocking (TdR) synchronization of HeLa cells. We then identify 6 gene clusters whose distal polyA site usages fluctuate during cell cycle.

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