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Next Generation Sequencing Analysis of Gene expression of Min6 insulinoma cell line transfected with siNC, siSRSF1 or siSRSF2

ABSTRACT: Next Generation Sequencing Analysis of Gene expression of Min6 insulinoma cell line transfected with siNC, siSRSF1 or siSRSF2

PROVIDER: PRJNA738590 | ENA |

REPOSITORIES: ENA

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Next Generation Sequencing Analysis of Gene expression of Min6 insulinoma cell line transfected with siNC, siSRSF1 or siSRSF2

Project description:Assessment of the impact of SRSF1 or SRSF2 silencing in Min6 cell lines

2024-04-28 | GSE178357 | GEO

Transcription profiling by array of mouse pancreatic beta cells of MIN6 insulinoma cell line transfected with siRNA directed against murine Meis3 or non targeting control.

Project description:In order to identify genes that are regulated by Meis3, MIN6 insulinoma cells were transfected with siRNA directed against murine Meis3 (siMeis3) or non targeting control (siControl). RNA was harvested and reverse transcribed. Reduced expression of Meis3 was confirmed. The RNA was then submitted for labeling and hybridization to the Agilent cDNA microarray.

2010-11-01 | E-MTAB-273 | biostudies-arrayexpress

Transcription profiling of mouse MIN6 insulinoma beta cells following knock-down of murine Pdx1

Project description:In order to identify genes that are regulated by Pdx1, MIN6 insulinoma cells were transduced with adenovirus expressing a short hairpin RNA directed against murine Pdx1 (AdshPdx1) or luciferase (AdshLuc) or not transduced at all (untreated). RNA was harvested and reverse transcribed. Reduced expression of Pdx1 and established Pdx1 targets was confirmed. The RNA was then submitted for labeling and hybridization to the PancChip cDNA microarray.

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Selenomethionine Modulates Insulin Secretion in the MIN6-K8 Mouse Insulinoma Cell Line

Project description:Selenomethionine Modulates Insulin Secretion in the MIN6-K8 Mouse Insulinoma Cell Line

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m6A-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

Project description:To further explore the underlying molecular mechanism of GR’s effects, we transfected the Min6 cell with the GR cDNA expression vector or control vector, and then extracted RNA of Min6 cells. M6A antibody was used to specifically recognize m6A on mRNA. methylation modified RNA fragments were obtained by immunoprecipitation and used for high-throughput sequencing. By comparing the captured RNA fragments, we found that there were 22381 difference peaks between GR overexpression group and control group, among which 3659 binding peaks were significantly different.Further analysis the MeRIP sequencing results of MIN6 cells overexpressing GR revealed that decreased m6A was detected in a large number of autophagy-related mRNAs.

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Mouse Insulinoma 6 (MIN6) transcriptome data at 2.8mM and 25mM extracellular glucose concentrations.

Project description:Mouse Insulinoma 6 (MIN6) transcriptome data at 2.8mM and 25mM extracellular glucose concentrations.

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Modulation of insulin secretion through RBFOX2-mediated alternative splicing [eCLIP-Seq MIN6]

Project description:Recent studies have identified dysregulation of RNA-binding proteins (RBPs) and aberrant mRNA splicing in the onset of diseases including diabetes. Here we investigated the role of RBFOX2 in the pancreatic β cell through the conditional mutation of Rbfox2 in the mouse pancreas (Pdx1:Cre; Rbfox2fl/lf) and RNAi experiments in the mouse insulinoma cell line, MIN6. We then identified the direct targets of RBFOX2 in the mouse β cell transcriptome my eCLIP-Seq in MIN6 cells.

2023-09-25 | GSE221276 | GEO

Modulation of insulin secretion through RBFOX2-mediated alternative splicing [RNA-seq MIN6]

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RNA-seq of Min6 cells were transfected with the GR cDNA expression vector or control vector

Project description:In order to investigated the underlying molecular mechanism of GR’s effects, we transfected the Min6 cell with the GR cDNA expression vector or control vector, and then extracted RNA of Min6 cells for RNA sequencing. We studied the gene-expression differences resulting in GR overexpress. A total of 21542 gene transcripts was captured,and 233 genes showed significant expression differences (p-value<0.05,|log2Foldchange|>1) between the two group. Further GO analysis and KEGG analysis found that GR overactivation instigates dysregulated autophagy in beta-cells

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RNA-Seq of mouse beta cell line MIN6 after siRNA knockdown of lncRNA Pax6os1

Project description:Pax6os1 was knocked using by a pool of 3 siRNAs in the mouse insulinoma (beta cell) line MIN6. A parallel experiment using 3 control siRNAs was performed and RNA extracted 72 hours after transfection and subjected to RNA-Seq.

2021-06-01 | E-MTAB-9218 | biostudies-arrayexpress

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