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Gibberellins regulate ovule number through a DELLA-CUC2 complex in Arabidopsis

ABSTRACT: Gibberellins regulate ovule number through a DELLA-CUC2 complex in Arabidopsis

PROVIDER: PRJNA738922 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14854962.fastq.gz	Fastqsanger.gz
	SRR14854963.fastq.gz	Fastqsanger.gz
	SRR14854964.fastq.gz	Fastqsanger.gz
	SRR14854965.fastq.gz	Fastqsanger.gz
	SRR14854966.fastq.gz	Fastqsanger.gz

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Similar Datasets

Gibberellins regulate ovule number through a DELLA-CUC2 complex in Arabidopsis

Project description:Ovule development is a key process for plant reproduction that ensures correct seed production. Understanding the molecular mechanisms that control ovule formation will also provide new approaches to increase crop yield for breeding. Several molecular factors and plant hormones, including gibberellins, are involved in ovule initiation and development. Gibberellins control ovule development by the destabilization of DELLA proteins, whereas DELLA activity has been proved to act as a positive factor for ovule primordia emergence. But the molecular mechanism by which DELLA act remained unknown. Here we have proved that DELLA proteins control ovule initiation by the formation of a protein complex with the CUC2 transcription factor. The DELLA protein GAI requires CUC2 to promote ovule primordia formation, thus GAI would function by its direct protein-protein interaction with CUC2 in cells of the placenta that determine the boundary regions between ovules during pistil development. Analysis of GAI-CUC2 interaction and colocalization in placenta support this hypothesis. Moreover, molecular analysis of the loci at which GAI protein may act as transcriptional co-regulators in a CUC2-dependent manner identified a subset of target genes that would be regulated by the GAI-CUC2 complex and contribute to regulate ovule primordia emergence.

2021-12-23 | GSE178414 | GEO

Cotton ovule development

Project description:Cotton ovule development, mutant vs wild type, Comparisons of DP16 0 dpa ovule Keywords: WildType vs Mutant

2005-11-03 | GSE3546 | GEO

DELLA protein direct targets in Arabidopsis

Project description:The aim of this study is to identify early DELLA protein-responsive genes using a Dexamethasone (DEX)-inducible system. Two transgenic lines were used: one induces the expression of a dominant, gibberellin non-responsive DELLA protein (rga-delta17); the other is a control line that carries the same vector, but lacks the rga-delta17 transgene. By comparing the gene expression changes in the line that expresses the rga-delta17 protein in the presence or absence of DEX it is possible to identify putative targets of DELLA proteins. An empty vector transgenic line was included in this study to identify genes that might be regulated by the DEX inducible system that are not dependent on the DELLA protein. Keywords: Dexamethasone treatment, gibberellin treatment, time course, transgene effect

2007-11-21 | GSE8741 | GEO

Transcription profiling of Arabidopsis seed and flowers to identify DELLA regulated transcripts

Project description:Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in Arabidopsis

2006-10-01 | E-MEXP-849 | biostudies-arrayexpress

Phosphorylation Promotes DELLA Activity by Enhancing Its Binding to Histone H2A at Target Chromatin in Arabidopsis

Project description:DELLA proteins are conserved master growth regulators that play a central role in controlling plant development in response to internal and environmental cues. DELLAs function as transcription regulators, which are recruited to target promoters by binding to transcription factors (TFs) and histone H2A via its GRAS domain. Recent studies showed that DELLA stability is regulated post-translationally via two mechanisms, phytohormone gibberellin-induced polyubiquitination for its rapid degradation, and Small Ubiquitin-like Modifier (SUMO)-conjugation to alter its accumulation. Moreover, DELLA activity is dynamically modulated by two distinct glycosylations: DELLA-TF interactions are enhanced by O-fucosylation, but inhibited by O-linked N-acetylglucosamine (O-GlcNAc) modification. However, the role of DELLA phosphorylation remains unclear. Here, we identified phosphorylation sites in REPRESSOR OF ga1-3 (RGA, an AtDELLA) purified from Arabidopsis by tandem mass spectrometry analysis, and showed that phosphorylation of the RGA LKS-peptide in the poly-S/T region enhances RGA-H2A interaction and RGA association with target promoters. Interestingly, phosphorylation does not affect RGA-TF interactions. Our study has uncovered that phosphorylation is a new regulatory mechanism of DELLA activity.

2024-08-08 | PXD046004 | Pride

DELLA targets in proliferating leaf tissue

Project description:Transcriptome changes 1h or 4h following DELLA stabilisation in microdissected fully proliferating Arabidopsis leaves

2013-08-01 | GSE49399 | GEO

della-regulation in darkness versus light-DELLAs contribute to skotomorphogenesis

Project description:rs09-09_della-dark - della-regulation in darkness versus light - Identification of DELLA-dependent downstream targets in darkness - Aim was to determine downstream target of DELLA proteins involved in skotomorphogenesis. Wt, ga1-3, ga1-3_rga_gai_rgl1_rgl2_rgl3 global seeds were sterilized, sown on MS agar plates then put for stratification for 3 days at 4°C. Plates were placed in growth cabinet at 22°C for 5 days in darkness or in continous light. Keywords: gene knock out

2011-06-13 | GSE22681 | GEO

The role of DELLA protein in tomato plant response to water-deficit stress

Project description:We previously showed that increased levels of the tomato DELLA protein PROCERA (PRO) promoted ABA responses in guard cells, including ABA-induced stomatal closure and gene expression. Thus we aimed to identifiy DELLA-regulated genes in guard cells in order to study the molecular mechanism by which DELLA promotes stomatal closure.

2021-09-09 | GSE143999 | GEO

Regulation of xylem fiber differentiation by gibberellins through DELLA-KNAT1 interaction

Project description:Regulation of xylem fiber differentiation by gibberellins through DELLA-KNAT1 interaction

| PRJNA505783 | ENA

Identification of early target genes of the DELLA protein GAI in etiolated seedlings

Project description:The hormones gibberellins (GA) control many aspects of plant growth and development thruough the whole life cycle of the plant. For instance, GA participate in the establishment of the skotomorphogenic developmental program that is triggered when seeds germinate in darkness, i.e. under the soil. Under these conditions seedlings appear etiolated and developmental features usually triggered by light are kept repressed. The GA signaling elements GAI and RGA have a major, partially redundant role in this process. These proteins belong to the DELLA family of transcriptional regulators and are destabilized by GA, acting as negative regulators of the pathway. In order to understand the molecular basis of the regulation of the skotomorphogenesis by GA, we sought to identify early target genes of the activity of GAI in etiolated seedlings. For that purpose we analyzed rapid, global changes in gene expression in response to a transient accummulation of a dominant version of GAI, gai-1, which is resistant to GA-induced destabilization.

2011-09-01 | GSE24253 | GEO

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