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Escherichia coli BW25113

ABSTRACT: Experimental evolution of trmD-deficient E. coli

PROVIDER: PRJNA739405 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14959400_1.fastq.gz	Fastqsanger.gz
	SRR14959400_2.fastq.gz	Fastqsanger.gz
	SRR14959401_1.fastq.gz	Fastqsanger.gz
	SRR14959401_2.fastq.gz	Fastqsanger.gz
	SRR14959402_1.fastq.gz	Fastqsanger.gz

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E. coli BW25113 frdC vs. E. coli BW25113 HW2

Project description:Glycerol is an attractive feedstock for biofuels since it accumulates as a byproduct during biodiesel operations; hence, it is interesting to consider converting glycerol to hydrogen using the formate hydrogen lyase system of Escherichia coli which converts pyruvate to hydrogen. Starting with Escherichia coli BW25113 frdC that lacks fumarate reductase to eliminate the negative effect of accumulated hydrogen on glycerol fermentation and by using both adaptive evolution and chemical mutagenesis combined with a selection method based on increased growth on glycerol, we obtained an improved strain, HW2, that produces 20-fold more hydrogen in glycerol medium (0.68 mmol/L/h) compared to that of frdC mutant. HW2 also grows 5-fold faster (0.25 1/h) than BW25113 frdC on glycerol, so it achieves a reasonable growth rate. Corroborating the increase in hydrogen production, glycerol dehydrogenase activity in HW2 increased 4-fold compared to BW25113 frdC. In addition, a whole-transcriptome study revealed that several pathways that would decrease hydrogen yields were repressed in HW2 (fbp, focA, and gatYZ) while a beneficial pathway, eno which encodes enolase was induced.

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Microarray analysis of RNase I-deficient Escherichia coli versus wild-type BW25113

Project description:The present study investigated the role(s) of RNase I (encoded by the rna gene) in Escherichia coli by comparative gene expression analysis of an rna mutant and the isogenic wild-type E. coli strain BW25113. The transcriptomic analysis aims to provide mechanistic insight into aberrant phenotypes observed in the RNase I-deficient mutant.

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Project description:Escherichia coli BW25113 is the parent strain of the Keio collection comprising nearly 4,000 single-gene deletion mutants. We report the complete 4,631,469-bp genome sequence of this strain and the key variations from the type strain E. coli MG1655.

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Project description:We conducted experimental evolution of E. coli a mutual cross-feeding coculture, composed of two genetically-engineered Escherichia coli populations, I- and L-, which lack genes for biosynthesis of Ile (ilvE gene) and Leu (leuB gene), respectively. These two populations grew in a coculture by complementing required nutrients each other. After 883 generations of evolution experiments, we measured evolutionary changes in transcriptome, and we found ABC transporters for the branched chain amino acids (Ile, Leu, and Val) were significantly increased in both I- and L-. Also, we got other various results that would be interesting to discuss.

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Escherichia coli BW25113 isolate:Population

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E. coli BW25113 frdC vs. E. coli BW25113 HW2

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Escherichia coli BW25113

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Transcriptional responses of Escherichia coli rpoS- BW25113 vs. wild-type BW25113 under 15% ethanol shock in log phase

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