Project description:Mrakia psychrophlila is a psychrophilic fungus widely spread all over the world. The cold adaptation mechanism of Mrakia psychrophila is unknown. Using RNA sequencing, differentially expressed genes of Mrakia psychrophila cultured at 4, 12 and 20 degree centigrade are identified. The result may be benefit for understanding cold adaptation of microorganisms.
Project description:Sequencing of 46 maize leaf whole transcriptome revealed 77 differentially expressed genes (DEGs) between Lancaster and Non-Lancaster maize genetic resources under optimal growing conditions. Cold test of the subset of four Lancaster and four Non-Lancaster lines showed that the first were cold sensitive and the later cold tolerant, with the majority of identified DEGs showing FPKM values above the plate mean in Lancaster, but below the plate mean in Non-Lancaster subset. Cold induced expression analysis for revealed that, among seven tested DEGs, ATP-sulfurylase and photosystem II I encoding genes showed responsiveness to low temperatures in a genetic-background-dependent manner, likely contributing to different cold response/adaptability of Lancaster and Non-Lancaster lines.
2020-12-31 | GSE164078 | GEO
Project description:Coffee leaves under optimal and warm temperatures
Project description:Here we use a transcriptomic approach to investigate the molecular underpinnings of thermal acclimation in the model diatom species Phaeodactylum tricornutum by comparing the differential gene expression in cultures acclimated to sub-optimal, optimal, and supra-optimal temperatures (10, 20 and 26.5 °C, respectively).
Project description:Comprehensive and quantitative information of the psychrophile proteome is an important source for understanding of the cold adaptation mechanism under low growth temperature. Mrakia psychrophila, a obligate fungus, was selected to quantitatively evaluate its protein abundance changes in response to three different temperatures. With optimized procedures of isobaric tags for relative and absolute quantitation quantitative proteomics(iTRAQ), high quality of the quantitative information of the peptides identified were acquired. In total, 1673 unique proteins were identified and defined 129 proteins as the temperature-dependent proteins. Function analysis revealed that energy metabolism is important for temperature adaptaion.
Project description:Cells in ectothermic organisms often maintain homeostatic function over a considerable range of ambient temperatures. However, as temperature has pronounced effects on all biological processes, but not necessarily in a uniform manner on each of the myriad of distinct processes, cellular acclimation to distinct temperatures is predicted to involve complex regulation. To assess the effects of a temperature downshift from 25 to 14°C, i.e. from the optimal temperature to the lower limit of the readily tolerated range, on the transcriptome in a time-resolved manner, we have performed expression profiling with S2R+ cells, which are derived from the ectothermic organism Drosophila melanogaster.
