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ABSTRACT: Mechanism of NAC in improving Follicle-stimulating Hormone Secretion

PROVIDER: PRJNA741083 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14902850_1.fastq.gz	Fastqsanger.gz
	SRR14902850_2.fastq.gz	Fastqsanger.gz
	SRR14902851_1.fastq.gz	Fastqsanger.gz
	SRR14902851_2.fastq.gz	Fastqsanger.gz
	SRR14902852_1.fastq.gz	Fastqsanger.gz

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Melatonin, as a downstream molecule of follicle-stimulating hormone, improves nutrient accumulation and mitochondrial function in mammalian oocytes.

Project description:Background: Melatonin (MLT) supplementation has been shown to improve the quality of oocytes and embryos in vitro through its antioxidant mechanism. However, it is unclear whether endogenous MLT plays a role in oocyte quality control, casting doubt on the rationality of oral MLT in improving oocyte quality or fecundity. Methods:we measured the effects of PMSG on MLT synthesis in follicles, evaluated the effects of MLT on oocyte growth and quality in mice and goat, used RNA-seq to explore the mechanism underlying MLT’s effects on oocyte growth and quality, and generated SNAT-knockout mice to assess the effects of endogenous MLT on oocyte growth and quality. Lastly, we investigated the feasibility of short-term MLT intake to improve the fertility of older mice. Result: we revealed that the follicle-stimulating hormone induces the expression of intra-ovarian SNAT, resulting in increased levels of intra-ovarian MLT in mice. Administering MLT during the gonadotropin-dependent phase improved the oocyte size and quality in both mice and goat by enhancing nutrient accumulation and mitochondrial function. Deficiency of MLT in mice reduced oocyte size and weakened mitochondrial function. Furthermore, our findings demonstrate that short-term MLT intake during the gonadotropin phase improves the litter size of older mice. Conclusion: This study reveals that endogenous MLT is involved in determining oocyte quality as a target molecule of follicle-stimulating hormone, shedding light on its potential utilization in improving oocyte quality or rejuvenating aged mammalian oocytes.

2023-08-12 | GSE240209 | GEO

RNA-Seq in porcine ovaries treated with recombinant pig follicle-stimulating hormone (rpFSH)

Project description:To identify the DE mRNA and lncRNA between the recombinant pig follicle-stimulating hormone traetment group and control grooup.

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Project description:Gene expression in follicle-stimulating hormone-treated rat Sertoli cells

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Intra-pituitary follicle-stimulating hormone signaling regulates hepatic lipid metabolism

Project description:Intra-pituitary follicle-stimulating hormone signaling regulates hepatic lipid metabolism

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Modeling FSHR function with hPSCs

Project description:Follicle-stimulating hormone (FSH) is crucial in the development and regulation of reproductive functions. We expressed MAC-tagged FSH receptor (FSHR) in HEK293 cells followed by affinity purification mass spectrometry analyses, to investigate interactome of FSHR at resting state and upon FSH stimulation. In total, 19 specific high-confidence interactors for WT FSHR and 14 for A189V FSHR, several of which have been linked to infertility.

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Hypoglycosylated FSH enhances oocyte quality via increased cell-to-cell interaction during mouse follicle development

Project description:Differences in follicle-stimulating hormone (FSH) ß-subunit N-glycosylation results in distinct FSH glycoforms. Hypoglycosylated FSH21 is more bioactive and abundant in reproductively young women, whereas fully glycosylated FSH24 shows lower bioactivity and a relative increase with age. To investigate if the coinciding shift in FSH glycoform abundance contributes to the age-dependent decline in oocyte quality, an encapsulated in vitro follicle growth system was utilized to examine the direct effects of FSH glycoforms on folliculogenesis and resulting oocyte quality. Long-term culture (10-12 days) with FSH21 (10 ng/ml) enhanced follicle growth, estradiol secretion, and oocyte quality compared to equivalent FSH24 treatment. FSH21 exhibited enhanced capacity to establish transzonal projections, gap junctions, and cell-to-cell communication within 24 hrs in culture. Transient inhibition of FSH21-mediated bidirectional communication abrogated the positive effects of FSH21 on follicle growth, estradiol secretion and oocyte quality. These findings indicate FSH21 promotes folliculogenesis and oocyte quality in vitro through increasing cell-to-cell communication early in folliculogenesis and that the shift in abundance from FSH21 to FSH24 with reproductive aging could contribute to the age-dependent decline in oocyte quality.

2023-07-15 | GSE236967 | GEO

Thyrotropin aggravates insulin resistance by promoting macrophage inflammation in subclinical hypothyroidism.

Project description:In subclinical hypothyroidism (SH), serum thyroid-stimulating hormone (TSH) correlated positively with insulin resistance; however, the precise mechanism is now unclear. Except for thyroid follicular epithelial cells, macrophages exhibit the highest level of TSHR expression. Thus, we wondered if TSH leads to insulin resistance by macrophages. We established thyroid-stimulating hormone receptor (Tshr) myeloid-specific knockout (TshrMKO) C57BL6 mice, and observed that TshrMKO mice showed improvement on high-fat diet (HFD) induced obesity and insulin resistance, compared to WT mice. TshrMKO animals showed decreased macrophages infiltration and M1 polarization in liver, adipose, and skeletal muscles. Co-culture experiments proved that Tshr deficient macrophages decreased gluconeogenesis in primary hepatocytes but increased glucose uptake in 3T3L1 adipocytes and L6 skeletal muscle cells through improving insulin signaling, versus WT. TSH promoted the release of Il6, Il1a and Il1b cytokines, which upregulated Egr1, Lcn2 and Socs3, reversed by the inhibitors Il6st and Il1ra. Lastly, EGR1 transcriptionally activated LCN2 and SOCS3. In conclusion, TSH promotes the secretion of IL6, IL1A, and IL1B via activating macrophage M1 polarization, which leads to that the upregulation of EGR1, transcriptionally activating LCN2 and SOCS3 and inducing insulin resistance in liver, adipose, and skeletal muscle in SH.

2025-07-09 | GSE275079 | GEO

Gene expression analysis of porcine granulosa cells treated in virtro with sulforaphane or follicle stimulating hormone

Project description:FSH is considered as the most critical regulatory factor for follicle growth and development. It is widely acknowledged that exogenous administration of FSH can effectively stimulate ovulation.However, these techniques have several drawbacks such as high cost and detrimental effects on hormone balance and embryo implantation.Sulforaphane (SFN) is a naturally occurring compound that belongs to the isothiocyanate group, which is predominant in cruciferous vegetables.Our previous research demonstrated SFN could inhibit hypoxia-evoked apoptosis in porcine GCs. Intriguingly, SFN exhibits a biphasic dose-response, stimulating cell growth at low doses in various mammalian cells. Therefore,to investigate the functional implications of sulforaphane in the regulation of granulosa cell proliferation, we conducted a gene expression profiling analysis using RNA-seq data, comparing SFN to follicle stimulating hormone (FSH).

2024-10-01 | GSE233365 | GEO

RNA-Seq in porcine ovaries treated with recombinant pig follicle-stimulating hormone (rpFSH)

Project description:RNA-Seq in porcine ovaries treated with recombinant pig follicle-stimulating hormone (rpFSH)

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Activating-transcription factor 3 stimulates follicle-stimulating hormone β transcription in vitro, but is dispensable for FSH production in murine gonadotropes in vivo

Project description:Follicle-stimulating hormone (FSH), a dimeric glycoprotein produced by pituitary gonadotrope cells, regulates spermatogenesis in males and ovarian follicle growth in females. Hypothalamic gonadotropin-releasing hormone (GnRH) stimulates FSHβ subunit gene (Fshb) transcription, though the underlying mechanisms are poorly understood. To address this gap in knowledge, we examined changes in pituitary gene expression in GnRH-deficient mice (hpg) treated with a regimen of exogenous GnRH that increases pituitary Fshb but not luteinizing hormone β (Lhb) mRNA levels. Activating transcription factor 3 (Atf3) was among the most upregulated genes. ATF3 can heterodimerize with members of the AP-1 family to regulate gene transcription. Co-expression of ATF3 with JunB stimulated murine Fshb, but not Lhb, promoter-reporter activity in homologous LβT2 cells. ATF3 also synergized with a constitutively active activin type I receptor to increase endogenous Fshb expression in these cells. Nevertheless, FSH production was intact in gonadotrope-specific Atf3 knockout mice (cKO) and control littermates. Ovarian follicle development, ovulation, and litter sizes were also equivalent between genotypes. Testis weights and sperm counts did not differ between cKO and control males. Following gonadectomy, increases in LH secretion were enhanced in cKO animals. Though FSH levels did not differ between genotypes, post-gonadectomy increases in pituitary Fshb and gonadotropin α subunit expression were more pronounced in cKO mice. These data indicate that ATF3 can selectively stimulate Fshb transcription in vitro but is not required for FSH production in vivo.

2023-06-28 | GSE220809 | GEO

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