Cigar tobacco leaves
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA741203 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:In this study, we have used the comparative transcriptomic and proteomic approaches to decipher the changes in genes and protein abundances in cigar tobacco leaves under LL. In this study, DEGs and DEPs related to glycolysis, starch and sucrose metabolism, tyrosine metabolism, photosynthesis-antenna proteins, and photosynthesis pathways are significantly enriched. This study offers novel insights into both transcriptome and proteome levels response mechanisms under different light intensities.
2022-08-26 | GSE211923 | GEO
Project description:bacterial community structure in the rhizosphere of cigar tobacco Raw sequence reads
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Project description:Using this microarray data, we identified 19898 probes (717 upregulated and 1512 downregulated in the mock- and MeJA-treated leaf samples at ‘1wk’ and ‘2wk’ stages; 17669 with differential expression in these samples), compared with the mock-treated sample at ‘0wk’ stage. This work aims to identify the genes related to MeJA-induced senescence of tobacco whole-plant, and found several genes differentially expressed between the mock- and MeJA-treated samples at the same stage.
2022-05-31 | GSE182794 | GEO
Project description:Comparative analysis of tobacco leaves transcriptomes unveils carotenoid pathway potentially determined the characteristics of aroma compounds in different environmental regions. Tobacco (Nicotiana tabacum) is a sensitive crop to environmental changes, and a tobacco with unique volatile aroma fractions always formed in specific ecological conditions. In order to investigate the differential expressed genes caused by environmental changes and reveal the formation mechanism of characteristics of tobacco in three different aroma tobacco regions of Guizhou Province, Agilent tobacco microarray was adapted for transcriptome comparison of tobacco leaves in medium aroma tobacco region Kaiyang and light aroma tobacco regions Weining and Tianzhu. Results showed that there was big difference among the gene expression profiles of tobacco leaves in different environmental conditions. A total of 517 differential expressed genes (DEGs) between Weining and Tianzhu were identified, while 733 and 1,005 genes differentially expressed between Longgang and another two tobacco regions Weining and Tianzhu, respectively. Compared with Longgang, up-regulated genes in Weining and Tianzhu were likely involved in secondary metabolism pathways, especially carotenoid pathway, including PHYTOENE SYNTHASE, PHYTOENE DEHYDROGENASE, LYCOPENE ε-CYCLASE, CAROTENOID β-HYDROXYLASE and CAROTENOID CLEAVAGE DIOXYGENASE 1 genes, while most down-regulated genes played important roles in response to temperature and light radiation, such as heat shock proteins. Gene Ontology and MapMan analyses demonstrated that the DEGs among different environmental regions were significantly enriched in light reaction of photosystem II, response of stimulus and secondary metabolism, suggesting they played crucial roles in environmental adaptation and accumulation of aroma compounds in tobacco plants. Through comprehensive transcriptome comparison, we not only identified several stress response genes in tobacco leaves from different environmental regions but also highlighted the importance of carotenoid pathway genes for characteristics of aroma compounds in specific growing regions. Our study primarily laid the foundation for further understanding the molecular mechanism of environmental adaptation of tobacco plants and molecular regulation of aroma substances in tobacco leaves.
2012-02-23 | GSE36008 | GEO
Project description:Low light (LL) or shading is detrimental one among other abiotic stresses that limit plant growth and suppress the crop plants' productivity. However, shading is one of the key cultivation techniques in cigar wrapper tobacco production. A gap of knowledge is present related to transcriptional and translational regulatory networks under such conditions. Herein, this work is planned for the integrative profiling of transcriptome and proteome along with some biochemical and physio-anatomical analyses under different light intensities [T200 (200 μmol m−2 s−1), T100 (100 μmol m−2 s−1), and T50 (50 μmol m−2 s−1)] to uncover the underlying molecular response mechanisms. The result showed that the leaf anatomical structure has a relationship with leaf photosynthetic capacity; as LL intensities (particularly T50) decreased the leaf thickness and thickness of palisade, and spongy tissues resulting in a lower photosynthesis rate and eventually decreased the plant biomass compared with T200. Furthermore, 3045 differentially expressed genes (DEGs) and 966 differentially expressed proteins (DEPs) were identified in the transcriptome and proteome of cigar tobacco. Furthermore, the integrative analysis between both omics revealed a total of 160 correlated DEGs and DEPs. The integrated analysis of both omics showed that the shared DEGs and DEPs were upregulated in photosynthesis-antenna proteins, photosynthesis, and defense/detoxification-related pathways. While the correlated DEGs and DEPs were downregulated in glycolysis, starch and sucrose metabolism, tyrosine metabolism, and mitochondrial electron transport chain pathways along with decreased activities of glyceraldehyde-3-phosphate dehydrogenase, starch phosphorylase, and pyruvate kinase enzymes related to glycolysis. These results showed that cigar tobacco efficiently utilized low light and reconfigured its energy metabolism. Collectively, this study can offer profound insights into the response mechanism at the physio-anatomical, biochemical, and molecular levels and showcases valuable resources of genes and proteins for future functional studies underlying LL response.
2022-09-01 | PXD036470 |
Project description:Metabolic activity of the microbial community greatly affects the quality of cigar tobacco leaves (CTLs). To improve the quality of CTLs, two extrinsic microbes (Acinetobacter sp. 1H8 and Acinetobacter indicus 3B2) were inoculated into CTLs. The quality of CTLs were significantly improved after fermentation. The content of solanone, 6-methyl-5-hepten-2-one, benzeneacetic acid, ethyl ester, cyclohexanone, octanal, acetophenone, and 3,5,5-trimethyl-2-cyclohexen-1-one were significantly increased after inoculated Acinetobacter sp. 1H8. The inoculation of Acinetobacter sp. 1H8 enhanced the normal evolutionary trend of bacterial community. The content of trimethyl-pyrazine, 2,6-dimethyl-pyrazine, and megastigmatrienone were significantly increased after inoculated Acinetobacter indicus 3B2. The inoculation of Acinetobacter indicus 3B2 completely changed the original bacterial community. Network analysis revealed that Acinetobacter was negatively correlated with Aquabacterium, positively correlated with Bacillus, and had significant correlations with many volatile flavor compounds. This work may be helpful for improving fermentation product quality by regulating microbial community, and gain insight into the microbial ecosystem.
| S-EPMC9248808 | biostudies-literature
Project description:Tobacco (Nicotiana tabacum L.) is an important cash crop, and the size of its leaves significantly influences both yield and quality. However, the upper part of tobacco leaves, due to its dense tissue structure, often faces issues such as narrow and thick leaves during the production of roasted cigarettes. These problems have a severe impact on the yield and quality of the upper leaf. Although the mechanism of leaf size regulation in Arabidopsis thaliana has been extensively studied, it remains unclear for tobacco. Therefore, this research aimed to investigate the role of the NtAN3 gene in regulating tobacco leaf size by utilizing the NC82 variety. The researchers created both an overexpression mutant (G27) and a silencing mutant (M21) of the NtAN3 gene and examined their impact on leaf size using cell morphology observation and transcriptome analysis. These research findings offer valuable insights for molecular breeding aimed at improving tobacco yield and enhancing the availability of upper leaves.
2023-10-31 | GSE246454 | GEO