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Soil

ABSTRACT: Soil Metagenomic assembly

PROVIDER: PRJNA742150 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14999215_1.fastq.gz	Fastqsanger.gz
	SRR14999215_2.fastq.gz	Fastqsanger.gz
	SRR14999216_1.fastq.gz	Fastqsanger.gz
	SRR14999216_2.fastq.gz	Fastqsanger.gz
	SRR14999217_1.fastq.gz	Fastqsanger.gz

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Project description:Soil transplant serves as a proxy to simulate climate change in realistic climate regimes. Here, we assessed the effects of climate warming and cooling on soil microbial communities, which are key drivers in Earth’s biogeochemical cycles, four years after soil transplant over large transects from northern (N site) to central (NC site) and southern China (NS site) and vice versa. Four years after soil transplant, soil nitrogen components, microbial biomass, community phylogenetic and functional structures were altered. Microbial functional diversity, measured by a metagenomic tool named GeoChip, and phylogenetic diversity are increased with temperature, while microbial biomass were similar or decreased. Nevertheless, the effects of climate change was overridden by maize cropping, underscoring the need to disentangle them in research. Mantel tests and canonical correspondence analysis (CCA) demonstrated that vegetation, climatic factors (e.g., temperature and precipitation), soil nitrogen components and CO2 efflux were significantly correlated to the microbial community composition. Further investigation unveiled strong correlations between carbon cycling genes and CO2 efflux in bare soil but not cropped soil, and between nitrogen cycling genes and nitrification, which provides mechanistic understanding of these microbe-mediated processes and empowers an interesting possibility of incorporating bacterial gene abundance in greenhouse gas emission modeling.

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