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Histone H3 proline 16 hydroxylation regulates mammalian gene expression

ABSTRACT: Histone H3 proline 16 hydroxylation regulates mammalian gene expression

PROVIDER: PRJNA742234 | ENA |

REPOSITORIES: ENA

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Histone H3 proline 16 hydroxylation regulates mammalian gene expression [CUT&RUN]

Project description:Histone H3 proline 16 hydroxylation regulates mammalian gene expression [CUT&RUN]

| PRJNA742238 | ENA

Histone H3 proline 16 hydroxylation regulates mammalian gene expression [RNA-Seq]

Project description:Histone H3 proline 16 hydroxylation regulates mammalian gene expression [RNA-Seq]

| PRJNA742239 | ENA

Histone H3 proline 16 hydroxylation regulates mammalian gene expression [RNA-Seq]

Project description:Histone posttranslational modifications (PTMs) are important for regulating various DNA-templated processes. Emerging evidence suggests a critical involvement of the previously less-explored histone PTMs in gene activity regulation. Here, we report the existence of a new histone PTM in the mammalian cells, hydroxylation of H3 proline 16 (H3P16-OH) catalyzed by EglN2, a proline hydroxylase. We show that EglN2-mediated H3P16-OH enhances direct binding of Lysine-Specific Demethylase 5A (KDM5A) with its substrate, H3 lysine 4 trimethylation (H3K4me3), resulting in enhanced chromatin recruitment of KDM5A and decrease of H3K4me3 at target genes. Genome-wide mapping by CUT&RUN in multiple mammalian cell models reveals a functional connection between EglN2, H3P16-OH, KDM5A and H3K4me3. Integrated analysis with RNA-Seq also identifies distinct subsets of genes that are regulated through H3P16-OH in different cell lines. This study therefore demonstrates a new player of the “histone code” by revealing a role of H3P16-OH in regulating gene expression in mammalian cells.

2022-08-02 | GSE179112 | GEO

Histone H3 proline 16 hydroxylation regulates mammalian gene expression [CUT&RUN]

2022-08-02 | GSE179108 | GEO

Histone H3 proline 16 hydroxylation regulates mammalian gene expression

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-08-02 | GSE179113 | GEO

Proline hydroxylation of ChREBP

Project description:This project investigated proline hydroxylation of ChREBP. Proline hydroxylation was investigated in flag-IP enriched protein extracts from ChREBP-flag overexpressing HEK293 cells (A) and in ChREBP-IP enriched mouse liver protein (male, C57BL6/J) (B).

2020-10-19 | PXD019137 | Pride

Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster

Project description:This SuperSeries is composed of the following subset Series: GSE32362: Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster (Illumina HumanMethylation450 BeadChip) GSE33129: Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster (Illumina HiSeq2000 sequencing) Refer to individual Series

2012-05-02 | E-GEOD-33130 | biostudies-arrayexpress

Proline hydroxylation of collagens in prolyl 4-hydroxylase mutant mice

Project description:Collagen from the skin of wild type and prolyl 4-hydroxylase mutant mice was extracted and proline hydroxylation of the collagen-derived peptides were analyzed by LC-MS/MS.

2018-04-10 | PXD008802 | Pride

Oxygen enhances antiviral innate immunity through maintenance of EGLN1-catalysed proline hydroxylation of IRF3

Project description:Oxygen is essential for aerobic organisms, but little is known about its role in antiviral immunity. Here, we report that during responses to viral infection, the hypoxic conditions repress antiviral-responsive genes independently of HIF signalling. EGLN1 was identified as a key mediator of the enhancement exerted by the oxygen on antiviral innate immune responses. Under sufficient oxygen conditions, EGLN1 maintains its prolyl hydroxylase activity to catalyse hydroxylation of IRF3 at proline 10. This modification enhances IRF3 phosphorylation, dimerisation, and nuclear translocation, leading to subsequent IRF3 activation. Furthermore, mice and zebrafish with Egln1 deletion, treatment with the EGLN inhibitor, FG4592, or mice carrying an Irf3 P10A mutation are more susceptible to viral infections. These findings not only reveal a direct link between oxygen and antiviral responses, but also provide insights into the mechanisms by which oxygen regulates innate immunity

2024-01-18 | GSE253181 | GEO

Oxygen enhances antiviral innate immunity through maintenance of EGLN1-catalysed proline hydroxylation of IRF3

Project description:Oxygen enhances antiviral innate immunity through maintenance of EGLN1-catalysed proline hydroxylation of IRF3

| PRJNA1064164 | ENA