Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Quantitative Analysis of PCB-exposed and Sham-exposed Rats Liver, Brain, Thymus and Spleen Transcriptomes

ABSTRACT: Next Generation Sequencing Quantitative Analysis of PCB-exposed and Sham-exposed Rats Liver, Brain, Thymus and Spleen Transcriptomes

PROVIDER: PRJNA742266 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14936600_1.fastq.gz	Fastqsanger.gz
	SRR14936600_2.fastq.gz	Fastqsanger.gz
	SRR14936601_1.fastq.gz	Fastqsanger.gz
	SRR14936601_2.fastq.gz	Fastqsanger.gz
	SRR14936602_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

Next Generation Sequencing Quantitative Analysis of PCB-exposed and Sham-exposed Rats Liver, Brain, Thymus and Spleen Transcriptomes

Project description:Our goal was to investigate the transcriptomes changes on rats liver, brain, thymus, and spleen after exposure to an indoor school air mixture (SAM+) of polychlorinated biphenyls (PCBs). Female Sprague-Dawley rats were exposed to SAM+ at a concentration of 45.5±5.9 µg/m^3 Σ209PCB or filtered air (sham group) 4 h/day, 6 days/week for 13 weeks using nose-only exposure systems. Twenty-four hours after the final exposure, rats were euthanized by carbon dioxide inhalation followed by cervical dislocation. Cardiac blood was collected and organs were excised and stored at -80 °C until analysis.

2021-06-30 | GSE179117 | GEO

Quantitative proteomics of liver from PCB 153 exposed Atlantic cod

Project description:In order to investigate the underlying mechanisms of PCB 153 mediated toxicity to Atlantic cod (Gadus morhua), we analyzed the liver proteome of fish exposed to various doses of PCB 153 (0, 0.5, 2 and 8mg/kg body weight) for two weeks and examined the effects on expression of liver proteins using quantitative proteomics. Label-free mass spectrometry enabled quantification of 1272 proteins, and 78 were differentially regulated between PCB 153 treated samples and controls. Two proteins downregulated due to PCB 153 treatment, Glutathione S-transferase theta 1 (GSTT1) and sulfotransferase family protein 1 (ST2B1), were verified using selected reaction monitoring (SRM). Supported by bioinformatics analyses, we concluded that PCB 153 perturbs lipid metabolism in the Atlantic cod liver and that increased levels of lipogenic enzymes indicate increased synthesis of fatty acids and triglycerides.

2017-01-17 | PXD004362 | Pride

RNA-seq analysis of spleen and liver tissue from wild-type and SXR/PXR knockout mice exposed to PCB-153 or vehicle

Project description:Purpose: to investigate transcriptomal differences between wild-type and SXR/PXR knockout mice and the impact of PCB-153 exposure to both strains to help reveal the mechanism behind the phenotype of hemolytic anemia observed in SXR knockout mice exposed to PCB-153

2019-08-17 | GSE135916 | GEO

RNAseq of zebrafish (Danio rerio) developmentally exposed to the polychlorinated biphenyl 3,3'-dichlorobiphenyl (PCB-11)

Project description:3,3’-Dichlorobiphenyl (PCB-11) is a non-legacy PCB congener widely detected in environmental samples and has been detected in human serum, but its toxicity potential is poorly understood. Zebrafish statically exposed to 4,500 µg/L PCB-11 at 24 hours post fertilization (hpf) were collected at 96 hpf for RNA sequencing.

2019-08-22 | GSE118955 | GEO

Transcriptional response to embryonic PCB exposure in 8 killifish populations

Project description:Embryos from 8 populations of killifish, four with evolved tolerances, were exposed to PCB-126. Approximately 5 replicate embryos per treatment/control were sequenced. Desensitization of aryl hydrocarbon receptor signaling to PCB-126 is found in all four populations with evolved pollution tolerance, and represents the strongest transcriptional difference between tolerant and sensitive populations.

2020-08-20 | GSE156460 | GEO

RNA-seq of wild-type iNKT1 cells from thymus, liver and spleen

Project description:The goal of this study was to compare the gene expression program of iNKT1 cells in the thymus, liver and spleen. To identify iNKT1 cells we created Tbx21Cre bacterial artifical chromosome transgenic mice with a Rosa26-floxed-stop-YFP reporter. We examined gene expression by RNA-sequencing in sorted iNKT1 cells (CD1d-Tet-PBS57+ YFP+) from the thymus, liver and spleen. Reads were aligned to the mm10 reference genome using Tophat v 2.1.0. Reads were assigned to genes using the htseq-count tool from HTSeq v 0.6.1 and gene annotations from Ensembl release 78. Differential expression was calculated across 3 independent replicates by EdgeR. We found that iNKT1 cells from all tissues had very similar gene expression programs but that there were subtle difference in each tissue that may have relevance for survival and function in these tissues.

2022-10-15 | GSE215338 | GEO

Strain-specific CNV in zebrafish treated with PCB-126, vehicle control, or untreated

Project description:Strain-specific CNV in zebrafish as determined by competitevly hybridizeing AB, Tuebingen, or WIK individuals exposed to PCB-126, vehicle control, or naïve versus pooled Casper strain

2019-02-12 | GSE110763 | GEO

Effects of PCB 52 Exposure Via Sub-Acute Nose-Only Inhalation on Gene Expression in Livers of Adolescent Rats

Project description:Polychlorinated biphenyls (PCBs) are persistent environmental toxicants that pose a variety of health risks in humans. PCB 52 has been found in the indoor air of schools where adolescent students and staff are being actively exposed. This study examined the livers of adolescent Sprague-Dawley rats after sub-acute exposure to PCB 52 via nose-only inhalation. Rats were exposed for 4 hours daily for 28 consecutive days. RNA extracted from both male and female livers were submitted for RNA sequencing to compare the effects of PCB 52 on the expression of genes in the liver.

2024-02-27 | GSE255048 | GEO

Proteomic characterization for thymus and spleen in immuno-suppressed mice with 10-hydroxydec-2-enoic acid treatment

Project description:In order to elucidate the molecular basis of 10-hydroxydec-2-enoic acid regulating immunity, immuno-suppressed mice were generated by cyclophosphamide induction, then treated by 10-HDA, and the proteomes of the thymus and spleen, the immuno-organs, were characterized with a mass spectrometry-based strategy.

2019-12-29 | PXD016902 |

RNA-seq analysis of spleen and liver tissue from wild-type and SXR/PXR knockout mice exposed to PCB-153 or vehicle

Project description:RNA-seq analysis of spleen and liver tissue from wild-type and SXR/PXR knockout mice exposed to PCB-153 or vehicle

| PRJNA560563 | ENA