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Total RNA transcriptome analysis of hypoxia with sgSETDB1 in hela cells

ABSTRACT: Total RNA transcriptome analysis of hypoxia with sgSETDB1 in hela cells

PROVIDER: PRJNA743293 | ENA |

REPOSITORIES: ENA

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Total RNA transcriptome analysis of hypoxia with sgSETDB1 in hela cells

Project description:Purpose: The goals of this study are to compare NGS-derived Hela with sgSETDB1 and shcontrol in hypoxia transcriptome profiling (RNA-seq) to quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods

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mRNA transcriptome analysis of hypoxia with shSETDB1 in hela cells

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mRNA transcriptome analysis of hypoxia with shSETDB1 in hela cells

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HeLa response to hypoxia

Project description:Oxygen is vital for the development and survival of mammals. In response to hypoxia, the brain initiates numerous adaptive responses at the organ level as well as at the molecular and cellular level, including the alteration of gene expression. Astrocytes play critical roles in the proper functioning of the brain, thus the manner in which astrocytes respond to hypoxia is likely important in determining the outcome of brain hypoxia. We used microarray gene expression profiling and data analysis algorithms to identify and analyze hypoxia-responsive genes in primary human astrocytes. We also compared the gene expression patterns in astrocytes with those in human HeLa cells and pulmonary artery endothelial cells (ECs). Remarkably, in astrocytes, 5 times as many genes were up-regulated as down-regulated, whereas in HeLa and pulmonary ECs, as many or more genes were down-regulated as up-regulated. More genes encoding hypoxia-inducible functions, such as glycolytic enzymes and angiogenic growth factors, were strongly induced in astrocytes compared to HeLa cells. The extent of induction was also greater than in HeLa cells. Further, gene ontology and computational algorithms revealed that many target genes of the EGF and insulin signaling pathways and the transcriptional regulators Myc, Jun and p53 were selectively altered by hypoxia in astrocytes and HeLa cells to a varying degree. These results provide a global view of the signaling and regulatory network mediating oxygen regulation in astrocytes, in comparison with that in HeLa cells. Keywords: response to hypoxia

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Analysis of hypoxia with sgSETDB1 in hela cells

Project description:Analysis of hypoxia with sgSETDB1 in hela cells

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Project description:Hypoxia is associated with several diseases, including cancer. Circular RNAs (circRNAs) are a novel class of non-coding RNAs that have been implicated in multiple cancers and attract increasing attention as potential biomarkers. In this study, the transcriptome-wide response of human HeLa cervical cancer cells to hypoxia was investigated. The circRNA signatures as well as differential gene expression and alternative splicing were analyzed by integrating available tools with custom approaches for quantification and statistical analysis.

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