Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional impact of EGFR-AT1R-TP receptor crosstalk in mouse aortic vascular smooth muscle cells

ABSTRACT: Transcriptional impact of EGFR-AT1R-TP receptor crosstalk in mouse aortic vascular smooth muscle cells

PROVIDER: PRJNA743534 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15035200_1.fastq.gz	Fastqsanger.gz
	SRR15035200_2.fastq.gz	Fastqsanger.gz
	SRR15035201_1.fastq.gz	Fastqsanger.gz
	SRR15035201_2.fastq.gz	Fastqsanger.gz
	SRR15035202_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 164

Similar Datasets

Transcriptional impact of EGFR-AT1R-TP receptor crosstalk in mouse aortic vascular smooth muscle cells

Project description:The impact EGFR, AT1R and TP receptor individual or simultaneous activations on the gene expression was investigated in mouse aortic vascular smooth muscle cells (aVSMC)

2022-06-16 | GSE179389 | GEO

Transcriptional impact of EGFR-AT1R-crosstalk

Project description:Transcriptional impact of EGFR-AT1R-crosstalk

| PRJNA682993 | ENA

Transcriptional impact of EGFR-AT1R-crosstalk (Transient transfection of AT1R in HEK293 cells)

Project description:Transcriptional impact of EGFR-AT1R-crosstalk (Transient transfection of AT1R in HEK293 cells)

| PRJNA682998 | ENA

Transcriptional impact of EGFR-AT1R-crosstalk (Stable transfection of AT1R in HEK293 cells)

Project description:Transcriptional impact of EGFR-AT1R-crosstalk (Stable transfection of AT1R in HEK293 cells)

| PRJNA682997 | ENA

Transcriptional impact of EGFR-AT1R-crosstalk (Transient transfection of AT1R in HEK293 cells)

Project description:The impact of AT1R and EGFR on SRF transcriptional activity, signaling pathways and transcriptome regulation were investigated in HEK293, HK-2 and vascular smooth muscle cells, applying reporter gene, protein and mRNA expression techniques as well as RNAseq followed by bioinfor-matic analysis.

2022-01-10 | GSE162769 | GEO

Transcriptional impact of EGFR-AT1R-crosstalk (Stable transfection of AT1R in HEK293 cells)

2022-01-10 | GSE162768 | GEO

Transcriptional impact of EGFR-AT1R-crosstalk

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-01-10 | GSE162770 | GEO

The Functional Interaction of EGFR with AT1R or TP in Primary Vascular Smooth Muscle Cells Triggers a Synergistic Regulation of Gene Expression.

Project description:In vivo, cells are simultaneously exposed to multiple stimuli whose effects are difficult to distinguish. Therefore, they are often investigated in experimental cell culture conditions where stimuli are applied separately. However, it cannot be presumed that their individual effects simply add up. As a proof-of-principle to address the relevance of transcriptional signaling synergy, we investigated the interplay of the Epidermal Growth Factor Receptor (EGFR) with the Angiotensin-II (AT1R) or the Thromboxane-A2 (TP) receptors in murine primary aortic vascular smooth muscle cells. Transcriptome analysis revealed that EGFR-AT1R or EGFR-TP simultaneous activations led to different patterns of regulated genes compared to individual receptor activations (qualitative synergy). Combined EGFR-TP activation also caused a variation of amplitude regulation for a defined set of genes (quantitative synergy), including vascular injury-relevant ones (Klf15 and Spp1). Moreover, Gene Ontology enrichment suggested that EGFR and TP-induced gene expression changes altered processes critical for vascular integrity, such as cell cycle and senescence. These bioinformatics predictions regarding the functional relevance of signaling synergy were experimentally confirmed. Therefore, by showing that the activation of more than one receptor can trigger a synergistic regulation of gene expression, our results epitomize the necessity to perform comprehensive network investigations, as the study of individual receptors may not be sufficient to understand their physiological or pathological impact.

| S-EPMC9222111 | biostudies-literature

Cutibacterium acnes strain:TP-CU426

Project description:TP-CU426

| PRJDB14092 | ENA

Mapping AT1R biased signaling using proximity-labeling and proteomics identifies diverse actions of biased agonists

Project description:Angiotensin II type 1 receptors (AT1Rs) are one of the most widely studied G protein-coupled receptors. To fully appreciate the diversity in cellular signaling profiles activated by AT1R transducer-biased ligands, we utilized peroxidase-catalyzed proximity labeling to capture proteins in close proximity to AT1Rs in response to six different ligands: Angiotensin II (full agonist), S1I8 (partial agonist), TRV055 and TRV056 (G protein-biased agonists), TRV026 and TRV027 (β-arrestin biased agonists) at 90s, 10min, and 60min after stimulation. We systematically analyzed the kinetics of AT1R trafficking and determined that distinct ligands lead AT1R to different cellular compartments for downstream signaling activation and receptor degradation/recycling. Distinct proximity labelling of proteins from a number of functional classes, including GTPases, adaptor proteins, and kinases were activated by different ligands suggesting unique signaling and physiological roles of the AT1R. Ligands within the same class, i.e. either G protein-biased or -arrestin-biased, shared high similarity in their labelling profiles. Comparison between ligand classes revealed distinct signaling activation such as greater labeling by G protein biased ligands on ESCRT-0 complex proteins that act as sorting machinery for ubiquitinated proteins. Our study provides a comprehensive analysis of AT1R receptor trafficking kinetics and signaling activation profiles induced by distinct classes of ligands.

2021-03-25 | PXD023814 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data