ABSTRACT: Multi-omics strategy uncovered the probiotic Lactiplantibacillus plantarum P9 regulated the gut microbial metabolites and alleviated inflammatory response in a pesticide exposure cohort
Project description:The study was conducted on a model of Lactiplantibacillus plantarum, one of the most studied species widely used in the food industry as a probiotic microorganism and/or microbial starter culture. As a result of step-by-step selection from the L. plantarum 8p-a3 strain isolated from the «Lactobacterin» probiotic, the L. plantarum 8p-a3-Clr-Amx strain was obtained, showing increased resistance, compared with the parent strain, to amoxicillin-clavulanic acid (MIC 20 mcg/ml) and clarithromycin (MIC 10 mcg/ml). The L. plantarum strain DMC-S1 was isolated from the intestine of Drosophila melanogaster Canton-S line. Extracellular vesicles of this bacterium can play a significant role in the drug-resistance development and host-microbe interactions.
Project description:The study evaluates the effect of Lactiplantibacillus plantarum IMC 510® supplementation on anthropometric and biochemical parameters, GM composition and gastrointestinal and general symptoms of overweight/obese subjects.
Project description:Using Drosophila we identified a glia-secreted antimicrobial peptide IM33, which alters gut microbiota to modulate sleep. To understand how the gut commensal Lactiplantibacillus plantarum functions in the brain to regulate sleep, we performed single-cell RNA sequencing.
Project description:The ability of Lactiplantibacillus plantarum LOC1 and LOC3, originally isolated from fresh tea leaves, to modulate the response of murine macrophages to the activation of Toll-like receptor 4 (TLR4) by the stimulation with lipopolysaccharide (LPS) was evaluated.
Project description:The aim of the study was to decipher metabolisms responsible (i) for the peculiar adaptation of L. plantarum during soy juice fermentation and (ii) for the release of aroma compounds, amino and short-chain fatty acid, and metabolites with health-promoting properties in soy yogurt. The strategy was the sequencing and annotation of a strain (L. plantarum CIRM-BIA777, PRJEB77707) able to degrade galacto- oligosaccharides, the sampling of soy yogurt, RNA-seq to identify differentially expressed genes of L. plantarum and corresponding metabolisms throughout the kinetics of fermentation. Acids and volatile compounds were also quantified.
Project description:Identification of proteins contained in extracellular vesicles of Lactiplantibacillus plantarum PCM 2675. Dataset is related to publication http://dx.doi.org/10.20517/evcna.2024.49. This work was financially supported by the National Science Centre, Poland (no. 2021/43/D/NZ6/01464).
