Project description:BDC2.5 mice treated with 1mg isotype control (2a3) or anti-CD4 (YTS177) antbodies for 48hrs. PLN CD4+ T cells sorter for microarray analysis.
Project description:Mice were immunized with either MOG35-55 in CFA or with CFA alone and 14 days later PLN cells were isolated and stimulated in vitro with MOG35-55 for 3 days followed by CD4+ T cell sorting. Microarray analysis of miRNA expression profiles was performed comparing sorted CD4+ T cells from MOG35-55 in CFA immunized mice to CD4+ T cells from mice immunized with CFA alone.
Project description:Exosomes were isolated from CD4+T cell by ExoQuickTC kit in rheumatoid arthritis patients and controls. Proteomic analysis of CD4+ T-cell-derived exosomes was performed by TMT combined with LC‒MS/MS.
Project description:Mice were immunized with either MOG35-55 in CFA or with CFA alone and 14 days later PLN cells were isolated and stimulated in vitro with MOG35-55 for 3 days followed by CD4+ T cell sorting. Microarray analysis of miRNA expression profiles was performed comparing sorted CD4+ T cells from MOG35-55 in CFA immunized mice to CD4+ T cells from mice immunized with CFA alone. The data are from magnetic beads sorted CD4+ T cells from peripheral lymph nodes from 5 pooled animals per data point
Project description:Local catabolism of the amino acid tryptophan (Trp) by indoleamine-2,3-dioxygenase (IDO) is considered an important mechanism of regulating T cell immunity. We show that IDO transcription was increased upon stimulation of myelin-specific T cells with tolerogenic altered self-peptides. Catabolites of Trp suppressed proliferation of myelin-specific T cells and inhibited production of proinflammatory TH1 cytokines. N-(3,4,-dimethoxycinnamoyl) anthranilic acid (3,4-DAA), an orally active synthetic derivative of the Trp metabolite anthranilic acid, reversed paralysis in mice with experimental autoimmune encephalomyelitis, a model of multiple sclerosis (MS). Trp catabolites and their derivatives offer a new strategy for treating TH1-mediated autoimmune diseases, such as MS. 4Y vd 1-11 comparison files are available as supplementary files. Keywords: 48hr activation, MBPAc1-11 Cd4+ T cell, murine Mu11ksubA and Mu11subB combined anaylsis