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Xenogeneic Skin Transplantation Promotes Angiogenesis and Tissue Regeneration

ABSTRACT: Xenogeneic Skin Transplantation Promotes Angiogenesis and Tissue Regeneration

PROVIDER: PRJNA744901 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15074031_1.fastq.gz	Fastqsanger.gz
	SRR15074031_2.fastq.gz	Fastqsanger.gz
	SRR15074032_1.fastq.gz	Fastqsanger.gz
	SRR15074032_2.fastq.gz	Fastqsanger.gz
	SRR15074033_1.fastq.gz	Fastqsanger.gz

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Xenogeneic Skin Transplantation Promotes Angiogenesis and Tissue Regeneration

Project description:1) We analyzed the impact of vitamind D3 (cholecalciferol) treatment on transcriptional signatures of bone marrow derived macrophages 2) Moreover, we analyzed cell populations infiltrating human split-thickness skin grafts (STSG) that had been subcutaneously implanted into wild-type mice (C57BL6/J).

2021-07-09 | GSE179745 | GEO

Xenogeneic skin transplantation promotes angiogenesis and tissue regeneration through activated Trem2<sup>+</sup> macrophages.

Project description:Skin allo- and xenotransplantation are the standard treatment for major burns when donor sites for autografts are not available. The relationship between the immune response to foreign grafts and their impact on wound healing has not been fully elucidated. Here, we investigated changes in collagen architecture after xenogeneic implantation of human biologic scaffolds. We show that collagen deposition in response to the implantation of human split-thickness skin grafts (hSTSGs) containing live cells recapitulates normal skin architecture, whereas human acellular dermal matrix (ADM) grafts led to a fibrotic collagen deposition. We show that macrophage differentiation in response to hSTSG implantation is driven toward regenerative Trem2+ subpopulations and found that hydrogel delivery of these cells significantly accelerated wound closure. Our study identifies the preclinical therapeutic potential of Trem2+ macrophages to mitigate fibrosis and promote wound healing, providing a novel effective strategy to develop advanced cell therapies for complex wounds.

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Skin regeneration is enabled in the absence of fibroblast inflammatory priming

Project description:Mammalian skin wounds heal by forming fibrotic scars. We report that reindeer antler velvet exhibits regenerative wound healing, whereas identical injury to back skin forms scar. This regenerative capacity was retained following ectopic transplantation of velvet to scar-forming sites. Single-cell mRNA/ATAC-Sequencing revealed that while uninjured velvet fibroblasts resembled human fetal fibroblasts, back skin fibroblasts were enriched in pro-inflammatory features resembling adult human fibroblasts. Injury elicited site-specific immune polarization; back skin fibroblasts amplified the immune response, whereas velvet fibroblasts adopted an immunosuppressive state leading to restrained myeloid maturation and hastened immune resolution ultimately enabling myofibroblast reversion to a regeneration-competent state. Finally, regeneration was blunted following application of back skin associated immunostimulatory signals or inhibition of pro-regenerative factors secreted exclusive to velvet fibroblasts. This study highlights a unique model to interrogate mechanisms underlying divergent healing outcomes and nominates both decoupling of stromal-immune crosstalk and reinforcement of pro-regenerative fibroblast programs to mitigate scar.

2023-02-23 | PXD035749 | Pride

Generation of allogenic and xenogeneic functional muscle stem cells for intramuscular transplantation [scRNA-seq]

Project description:Satellite cells, the stem cells of skeletal muscle tissue, hold a remarkable regeneration capacity and therapeutic potential in regenerative medicine. However, low satellite cell yield from autologous or donor-derived muscles hinders the adoption of satellite cell transplantation for the treatment of muscle diseases, including Duchenne muscular dystrophy (DMD). To address this limitation, here we investigated whether satellite cells can be derived in allogeneic or xenogeneic animal hosts. First, injection of CRISPR/Cas9-corrected mouse DMD-induced pluripotent stem cells (iPSCs) into mouse blastocysts carrying an ablation system of host satellite cells gave rise to intraspecies chimeras exclusively carrying iPSC-derived satellite cells. Furthermore, injection of genetically corrected DMD-iPSCs into rat blastocysts resulted in the formation of interspecies rat-mouse chimeras harboring mouse satellite cells. Remarkably, iPSC-derived satellite cells or derivative myoblasts produced in intraspecies or interspecies chimeras restored dystrophin expression in DMD mice following intramuscular transplantation, and contributed to the satellite cell pool. Collectively, this study demonstrates the feasibility of producing therapeutically competent stem cells across divergent animal species, raising the possibility of generating human muscle stem cells in large animals for regenerative medicine purposes.

2024-04-25 | GSE254371 | GEO

Generation of allogenic and xenogeneic functional muscle stem cells for intramuscular transplantation [RNA-seq]

2024-04-25 | GSE215061 | GEO

Generation of allogenic and xenogeneic functional muscle stem cells for intramuscular transplantation

Project description:Generation of allogenic and xenogeneic functional muscle stem cells for intramuscular transplantation

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Proteomic Analysis in Scar-Free Skin Regeneration in Acomys cahirinus

Project description:Comparative Proteomic Analysis in Scar-Free Skin Regeneration in Acomys cahirinus and Scarring Mus musculus

2019-11-24 | MSV000084615 | MassIVE

TNF-α-Activated Adipose–Derived Stem Cells Improve the Angiogenesis of Full-Thickness Skin Grafts Through the TNF-α/NF-κB Signaling Pathway

Project description:The repair of skin and soft tissue defects has been a long-standing topic of interest in plastic surgery. Skin grafts and flaps are the most commonly used methods for such repair, but both have some disadvantages. If a high-quality skin flap transplantation outcome could be obtained through simple skin grafting, it would surely benefit a majority of patients. Transplantation of adipose-derived stem cells (ADSCs) into skin and soft tissue wounds is a promising “therapeutic angiogenesis” strategy in this context. In the present study, we used TNF-α to mobilize ADSCs for the tissue regenerative process. Then, we injected the TNF-α-activated ADSCs intradermally into the donor skin of full-thickness skin grafts (FTSGs). TNF-α may activate ADSCs through the TNF-α/NF-κB pathway and enhance the ability of ADSCs to signal the paracrine secretion of the angiogenic factor IL-8, ultimately promoting the angiogenesis of donor skin. The use of vasculature-rich donor skin for grafting presented in our study could accelerate skin graft anastomosis; shorten the nutrient deprivation time of the epidermis, hair follicles, and dermis; and thereby improve the survival of FTSGs. Overall, the findings of the present study demonstrate a possible mechanism through which TNF-α acts on ADSCs to improve their angiogenic capacity and provide a novel approach for the repair of skin and soft tissue wounds.

2023-02-27 | GSE226232 | GEO

Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration

Project description:Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration

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Generation of allogenic and xenogeneic functional muscle stem cells for intramuscular transplantation [RNA-seq]

Project description:Generation of allogenic and xenogeneic functional muscle stem cells for intramuscular transplantation [RNA-seq]

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