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Transcriptome m6A sequencing (MeRIP-seq)of human pancreatic cancer cells

ABSTRACT: Transcriptome m6A sequencing (MeRIP-seq)of human pancreatic cancer cells

PROVIDER: PRJNA745458 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15097653_1.fastq.gz	Fastqsanger.gz
	SRR15097653_2.fastq.gz	Fastqsanger.gz
	SRR15097654_1.fastq.gz	Fastqsanger.gz
	SRR15097654_2.fastq.gz	Fastqsanger.gz
	SRR15097655_1.fastq.gz	Fastqsanger.gz

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Transcriptome m6A sequencing (MeRIP-seq)of human pancreatic cancer cells

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Project description:Background: Abdominal aortic aneurysm (AAA) is a vascular disease with indeterminate prevalence but high mortality rates when complicated with rupture. The pathogenesis of AAA has not been fully elucidated. N6-methyladenosine (m6A) modification is likely important in the development of AAA. In the present study, m6A-MeRIP sequencing and RNA sequencing were performed to identify the m6A sites. Bioinformatics analysis was used to evaluate the m6A patterns of the aorta walls of AngII-induced abdominal aortic aneurysm (AAA) model and normal mice. Results: There were 2039 differentially methylated m6A peaks involving 1865 mRNAs in the AAA group relative to the control, of which 1610 peaks in 1466 mRNAs were hypermethylated and 429 peaks in 410 mRNAs were hypomethylated. The hypermethylated mRNAs in AAA group were mostly enriched in transcription regulation and intercellular signaling, especially the Wnt signaling-associated processes. Hypomethylated m6A sites were mainly enriched in G protein-coupled receptor activity and ion channel activity. Conclusion: Our study suggested an original viewpoint that AAA might mainly be relevant to combined effect of m6A methylation modification in Wnt pathway, G protein-coupled receptor and ion channel- associated genes, which were worthy of further investigation.

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