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RNA Sequencing of livers from ApoE-/- mice treated with HIF1a inhibitor PX-478 or Saline

ABSTRACT: RNA Sequencing of livers from ApoE-/- mice treated with HIF1a inhibitor PX-478 or Saline

PROVIDER: PRJNA745978 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15107873_1.fastq.gz	Fastqsanger.gz
	SRR15107873_2.fastq.gz	Fastqsanger.gz
	SRR15107874_1.fastq.gz	Fastqsanger.gz
	SRR15107874_2.fastq.gz	Fastqsanger.gz
	SRR15107875_1.fastq.gz	Fastqsanger.gz

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RNA Sequencing of livers from ApoE-/- mice treated with HIF1a inhibitor PX-478 or Saline

Project description:Purpose: HIF1a inhibitor PX-478 prevents hypercholesterolemia in ApoE-/- mice fed Western diet for 3 months. The goal of this study was to determine the mechanisms in the liver through which PX-478 regulates cholesterol. Methods: 4-week old ApoE-/- mice were fed a Western diet and injected intraperitoneally, bi-weekly with either PX-478 (40 mg/kg) or Saline for 3 months. The livers of 3 PX-478-treated mice and 4 Saline-treated mice were used for this RNA-seq study. Results: We identified 800+ differentially expressed genes in the PX-478-treated ApoE-/- mice livers relative to the saline-treated control livers. A subset of these genes were found to play important roles in cholesterol regulation and were further validated by qPCR analysis. Conclusions: Our study details potential hepatic cholesterol regulating mechanisms for HIF1a inhibitor PX-478. These results, coupled with our findings on the effect of PX-478 on two different mouse models of atherosclerosis, demonstrate that PX-478 is a potential anti-atherogenic and anti-hypercholesterolemic drug.

2022-02-18 | GSE179952 | GEO

Sinorhizobium medicae strain:478

Project description:Sinorhizobium medicae 478 Resequencing

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Pseudomonas sp. 478

Project description:Pseudomonas sp. 478 Genome sequencing and assembly

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Edwardsiella ictaluri strain:S17-478

Project description:Edwardsiella ictaluri S17-478 Genome sequencing and assembly

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Host cell gene expression in Influenza A virus (A/Singapore/478/2009 (pH1N1)) infected A549 cells at 2, 4, 6, 8 and 10 hours post infection

Project description:We used the microarray data to analyze host cells response on A549 cells infected with Influenza A virus (A/Singapore/478/2009 (pH1N1)) The Influenza A virus (A/Singapore/478/2009 (pH1N1)) infected A549 cells were harvested at 2, 4, 6, 8 and 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A virus (A/Singapore/478/2009 (pH1N1)) infection.

2013-01-03 | E-GEOD-31518 | biostudies-arrayexpress

SF3B1 promotes glycolysis and tumor malignancy through splicing-independent activation of HIF1α [RNA-Seq murine organoids]

Project description:Splicing factor 3b subunit 1 (SF3B1) is one of the most frequently mutated spliceosomal components in cancer. Recent studies additionally imply an involvement of the wildtype protein in tumor progression, but the underlaying mechanism remains elusive. Here we report that SF3B1 acts as a coactivator for hypoxia-inducible factor (HIF) 1α. SF3B1 directly interacts with HIF1a, augments HIF1a-HIF1b heterodimer binding to hypoxia response elements in vivo and enhances transactivation of HIF target genes. In human patients, primary patient cells and in a mouse model of pancreatic ductal adenocarcinoma (PDAC), efficient HIF1a-signalling relies on SF3B1. Monoallelic deletion of Sf3b1 in this mouse tumor model severely delays tumor initiation and development and diminishes HIF target gene transcription, glucose uptake and proliferation in corresponding tumor organoids in hypoxia, while splicing remains functional. Here, we define a subset of aggressive chromophobe renal cell carcinomas (chRCCs) able to transcriptionally compensate monoallelic SF3B1 loss, a feature that correlates with poor prognosis and lymph node spread and we show that the oncogenic hotspot mutation SF3B1K700E has reduced co-activation efficiency. Also, we report increased sensitivity of cells with low levels of SF3B1 to the HIF-inhibitor PX-478. These findings reveal a splicing-independent, tumor promoting function of SF3B1, important in the pathogenesis of PDAC and other cancers.

2022-06-25 | GSE206703 | GEO

SF3B1 promotes glycolysis and tumor malignancy through splicing-independent activation of HIF1α [ChIP-Seq]

2022-06-22 | GSE201461 | GEO

SF3B1 promotes glycolysis and tumor malignancy through splicing-independent activation of HIF1α [RNA-Seq]

2022-06-22 | GSE201427 | GEO

Sex differences in statin response in hypercholesterolemic (Apoe knockout) Four Core Genotypes mice.

Project description:RNA-sequening of livers from Apoe knockout Four Core Genotypes mice (XX ovaries, XX testes, XY ovaries, XY testes) fed either chow diet or chow diet + simvastatin (0.1g/kg) for 8 weeks.

2022-08-11 | GSE202977 | GEO

Gene expression profiling in WT and Hif1a-/- HSCs

Project description:To understand the underlying mechanism by which the Hif1a gene is required by hematopoietic stem cells (HSCs), we performed a comparative DNA microarray analysis using total RNA isolated from wild type Lin-Sca-1+c-Kit+ cells and Hif1a-/- Lin-Sca-1+c-Kit+ cells. The result was validated by quantitative real-time PCR analysis of wild type Lin-Sca-1+c-Kit+ and Hif1a-/- Lin-Sca-1+c-Kit+ cells. We compared the gene profile of HSCs between WT mice and Hif1a-/- mice.

2012-04-03 | E-GEOD-35110 | biostudies-arrayexpress

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